Team:ETH Zurich/labblog/20140618meet

Week 4

Wednesday, June 18th

• We improved a little bit the plasmid design, determined which parts will be taken from the iGEM registry, and which parts we have to synthesize.

First draft of plasmids. The sensor plasmid is the same in every cell. Different logic + repressors plasmids are present in cells p and q. The logic and repressors plasmid is producing LuxI for cells p, and it is producing RhlI for cells q. Colonies of p and q cells are arranged in an alternate way on the millifluidic chip.

Sensor plasmids will be approx. 9.3kb long, and logic plasmids will be approx. 5,2kb long.

• From the modeling side, the whole model has been modularized and we have calculated and simulated steady state for the quorum sensing module.
• GeneScript, Microsynth, EraSynbio are willing to support us !

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