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Week 3
Wednesday, June 11th
- We started plasmid design :
ΦC31 and Bxb1 are integrases. LuxR an RhlR are quorum sensing molecules. A riboswitch construct is placed around quorum sensing constructs to prevent leakiness of lux and rhl promoters. Type p colonies produce AHL by expressing the enzyme LuxI. Type q colonies produce Rhl by expressing the enzyme RhlI. In fact, we don't know yet which quorum sensing systems we will use. We will have to perform cross-talk experimetns in order to choose the ones that are the most orthogonal.
- We tried to print our first agar millifluidic chip : we printed it too small, and the printer had resolution problems.
- We wrote all reactions and found parameters from the literature for our model.
