Team:ETH Zurich/lab/sequences
From 2014.igem.org
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[https://static.igem.org/mediawiki/2014/4/43/ETH2014_piG0049.txt piG0049] | [https://static.igem.org/mediawiki/2014/4/43/ETH2014_piG0049.txt piG0049] | ||
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+ | LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasI produces the quorum sensing molecule 3OC12-HSL. | ||
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[https://static.igem.org/mediawiki/2014/b/bc/ETH2014_piG0049max.txt piG0049max] | [https://static.igem.org/mediawiki/2014/b/bc/ETH2014_piG0049max.txt piG0049max] | ||
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+ | LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LasI ([https://salis.psu.edu/software/forward RBS calculator]). LasI produces the quorum sensing molecule 3OC12-HSL. | ||
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[https://static.igem.org/mediawiki/2014/4/49/ETH2014_piG0050.txt piG0050] | [https://static.igem.org/mediawiki/2014/4/49/ETH2014_piG0050.txt piG0050] |
Revision as of 16:58, 10 October 2014
Sequences
Regulator Constructs
LasR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR.
LuxR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlR (RBS calculator). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
LuxR is expressed under the weak constitutive promoter BBa_J23109 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
LuxR is expressed under the medium strong constitutive promoter BBa_J23111 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
Producer Constructs
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasI produces the quorum sensing molecule 3OC12-HSL.
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LasI (RBS calculator). LasI produces the quorum sensing molecule 3OC12-HSL.