Team:ETH Zurich/lab/sequences
From 2014.igem.org
Line 6: | Line 6: | ||
[https://static.igem.org/mediawiki/2014/f/f6/ETH2014_piG0040.txt piG0040] | [https://static.igem.org/mediawiki/2014/f/f6/ETH2014_piG0040.txt piG0040] | ||
+ | |||
LasR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. | LasR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. | ||
[https://static.igem.org/mediawiki/2014/2/2c/ETH2014_piG0041.txt piG0041] | [https://static.igem.org/mediawiki/2014/2/2c/ETH2014_piG0041.txt piG0041] | ||
+ | |||
LuxR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. | LuxR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. | ||
[https://static.igem.org/mediawiki/2014/d/db/ETH2014_piG0042.txt piG0042] | [https://static.igem.org/mediawiki/2014/d/db/ETH2014_piG0042.txt piG0042] | ||
+ | |||
RhlR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | RhlR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | ||
[https://static.igem.org/mediawiki/2014/c/ce/ETH2014_piG0042max.txt piG0042max] | [https://static.igem.org/mediawiki/2014/c/ce/ETH2014_piG0042max.txt piG0042max] | ||
+ | |||
RhlR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR ([https://salis.psu.edu/software/forward RBS calculator]). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | RhlR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR ([https://salis.psu.edu/software/forward RBS calculator]). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | ||
Revision as of 16:30, 10 October 2014
Sequences
Regulator Constructs
LasR is expressed under the constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR.
LuxR is expressed under the constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
RhlR is expressed under the constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
RhlR is expressed under the constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlR (RBS calculator). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
Producer Constructs