Team:ETH Zurich/lab/sequences

From 2014.igem.org

(Difference between revisions)
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[http://2014.igem.org/wiki/images/4/49/ETH2014_piG0050.txt piG0050]
[http://2014.igem.org/wiki/images/4/49/ETH2014_piG0050.txt piG0050]
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] while the expression level is influenced by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
   
   
[[File:ETH2014_piG0050_Map.png|link=http://2014.igem.org/wiki/images/4/49/ETH2014_piG0050.txt]]
[[File:ETH2014_piG0050_Map.png|link=http://2014.igem.org/wiki/images/4/49/ETH2014_piG0050.txt]]
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[http://2014.igem.org/wiki/images/5/50/ETH2014_piG0050max.txt piG0050max]
[http://2014.igem.org/wiki/images/5/50/ETH2014_piG0050max.txt piG0050max]
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LuxI ([https://salis.psu.edu/software/forward RBS calculator]). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] while the expression level is increased by an RBS optimised for LuxI ([https://salis.psu.edu/software/forward RBS calculator]). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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[http://2014.igem.org/wiki/images/0/00/ETH2014_piG0051.txt piG0051]
[http://2014.igem.org/wiki/images/0/00/ETH2014_piG0051.txt piG0051]
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] while the expression level is influenced by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
   
   
[[File:ETH2014_piG0051_Map.png|link=http://2014.igem.org/wiki/images/0/00/ETH2014_piG0051.txt]]
[[File:ETH2014_piG0051_Map.png|link=http://2014.igem.org/wiki/images/0/00/ETH2014_piG0051.txt]]
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[http://2014.igem.org/wiki/images/9/94/ETH2014_piG0051max.txt piG0051max]
[http://2014.igem.org/wiki/images/9/94/ETH2014_piG0051max.txt piG0051max]
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for RhlI ([https://salis.psu.edu/software/forward RBS calculator]). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] while the expression level is increased by an RBS optimised for RhlI ([https://salis.psu.edu/software/forward RBS calculator]). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
[[File:ETH2014_piG0051max_Map.png|link=http://2014.igem.org/wiki/images/9/94/ETH2014_piG0051max.txt]]
[[File:ETH2014_piG0051max_Map.png|link=http://2014.igem.org/wiki/images/9/94/ETH2014_piG0051max.txt]]

Revision as of 20:58, 17 October 2014

iGEM ETH Zurich 2014