# Team:ETH Zurich/lab/sequences

(Difference between revisions)
 Revision as of 20:50, 17 October 2014 (view source)Meistefa (Talk | contribs)← Older edit Revision as of 20:56, 17 October 2014 (view source)Meistefa (Talk | contribs) Newer edit → Line 25: Line 25: [http://2014.igem.org/wiki/images/f/f6/ETH2014_piG0040.txt piG0040] [http://2014.igem.org/wiki/images/f/f6/ETH2014_piG0040.txt piG0040] - LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. + LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] while the expression level is influenced by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. [[File:ETH2014_piG0040_Map.png|link=http://2014.igem.org/wiki/images/f/f6/ETH2014_piG0040.txt]] [[File:ETH2014_piG0040_Map.png|link=http://2014.igem.org/wiki/images/f/f6/ETH2014_piG0040.txt]] Line 32: Line 32: [http://2014.igem.org/wiki/images/2/2c/ETH2014_piG0041.txt piG0041] [http://2014.igem.org/wiki/images/2/2c/ETH2014_piG0041.txt piG0041] - LuxR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. + LuxR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] while the expression level is influenced by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. Line 40: Line 40: [http://2014.igem.org/wiki/images/d/db/ETH2014_piG0042.txt piG0042] [http://2014.igem.org/wiki/images/d/db/ETH2014_piG0042.txt piG0042] - RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. + RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] while the expression level is influenced by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. Line 48: Line 48: [http://2014.igem.org/wiki/images/c/ce/ETH2014_piG0042max.txt piG0042max] [http://2014.igem.org/wiki/images/c/ce/ETH2014_piG0042max.txt piG0042max] - RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR ([https://salis.psu.edu/software/forward RBS calculator]). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. + RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] while the expression level is influenced by an RBS optimised for RhlR ([https://salis.psu.edu/software/forward RBS calculator]). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. Line 56: Line 56: [http://2014.igem.org/wiki/images/1/18/ETH2014_piG0046.txt piG0046] [http://2014.igem.org/wiki/images/1/18/ETH2014_piG0046.txt piG0046] - LuxR is expressed under the weak constitutive promoter [http://parts.igem.org/Part:BBa_J23109 BBa_J23109] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. + LuxR is expressed under the weak constitutive promoter [http://parts.igem.org/Part:BBa_J23109 BBa_J23109] while the expression level is influenced by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. Line 64: Line 64: [http://2014.igem.org/wiki/images/1/1b/ETH2014_piG0047.txt piG0047] [http://2014.igem.org/wiki/images/1/1b/ETH2014_piG0047.txt piG0047] - LuxR is expressed under the medium strong constitutive promoter [http://parts.igem.org/Part:BBa_J23111 BBa_J23111 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. + LuxR is expressed under the medium strong constitutive promoter [http://parts.igem.org/Part:BBa_J23111 BBa_J23111 ] while the expression level is influenced by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20[[Team:ETH_Zurich/project/references#refBolivar|[15]]]. Line 73: Line 73: [http://2014.igem.org/wiki/images/4/43/ETH2014_piG0049.txt piG0049] [http://2014.igem.org/wiki/images/4/43/ETH2014_piG0049.txt piG0049] - LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [[Team:ETH_Zurich/project/references#refLennen|[16]]]. + LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] while the expression level is influenced by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [[Team:ETH_Zurich/project/references#refLennen|[16]]]. Line 81: Line 81: [http://2014.igem.org/wiki/images/b/bc/ETH2014_piG0049max.txt piG0049max] [http://2014.igem.org/wiki/images/b/bc/ETH2014_piG0049max.txt piG0049max] - LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LasI ([https://salis.psu.edu/software/forward RBS calculator]). LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [[Team:ETH_Zurich/project/references#refLennen|[16]]]. + LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] while the expression level is increased by an RBS optimised for LasI ([https://salis.psu.edu/software/forward RBS calculator]). LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [[Team:ETH_Zurich/project/references#refLennen|[16]]]. Line 186: Line 186: [http://2014.igem.org/wiki/images/f/f5/ETH2014_piG0096_pluxRRR12y-sfGFP-maxRBS-luxI.txt piG0096] [http://2014.igem.org/wiki/images/f/f5/ETH2014_piG0096_pluxRRR12y-sfGFP-maxRBS-luxI.txt piG0096] - Expression of sfGFP and LuxI is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The cis-repressive element (crR12y) inhibits the translation of the succeeding gene, since the RBS is blocked by secondary structures of the mRNA. The transcript of the trans-activating element (taR12y) binds to the transcript of the cis-repressive element, hence the RBS is not blocked anymore. The two elements build a [http://2014.igem.org/Team:ETH_Zurich/expresults#Riboregulators riboregulator] that decreases leakiness of pLuxR. The p15A is present at a copy number of approximately 15 to 25[[Team:ETH_Zurich/project/references#refChang|[35]]]. + Expression of sfGFP and LuxI is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The cis-repressive element (crR12y) inhibits the translation of the succeeding gene, since the RBS is blocked by secondary structures of the mRNA. The transcript of the trans-activating element (taR12y) binds to the transcript of the cis-repressive element, hence the RBS is not blocked anymore. The two elements build a [http://2014.igem.org/Team:ETH_Zurich/expresults#Riboregulators riboregulator] that decreases leakiness of pLuxR. The expression level of LuxI is increased by an RBS optimised for LuxI ([https://salis.psu.edu/software/forward RBS calculator]).The p15A is present at a copy number of approximately 15 to 25[[Team:ETH_Zurich/project/references#refChang|[35]]]. [[File:ETH2014 piG0096 pluxRRR12y-sfGFP-maxRBS-luxI Map.png|link=http://2014.igem.org/wiki/images/f/f5/ETH2014_piG0096_pluxRRR12y-sfGFP-maxRBS-luxI.txt]] [[File:ETH2014 piG0096 pluxRRR12y-sfGFP-maxRBS-luxI Map.png|link=http://2014.igem.org/wiki/images/f/f5/ETH2014_piG0096_pluxRRR12y-sfGFP-maxRBS-luxI.txt]]

## Revision as of 20:56, 17 October 2014

iGEM ETH Zurich 2014