Team:ETH Zurich/lab/sequences

From 2014.igem.org

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(Sensor Constructs)
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[http://2014.igem.org/wiki/images/5/59/ETH2014_piG0067_logic_bxb1_BUFFER-sfGFP.txt piG0067]
[http://2014.igem.org/wiki/images/5/59/ETH2014_piG0067_logic_bxb1_BUFFER-sfGFP.txt piG0067]
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The directional terminator [http://parts.igem.org/Part:BBa_B0015 BBa_B0015] blocks transcription of sfGFP under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100]. Only if the integrase Bxb1 flips B0015 between the attP and the attB sites, transcription of sfGFP is possible. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>
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[[File:ETH2014 piG0067 logic bxb1 BUFFER-sfGFP Map.png|link=http://2014.igem.org/wiki/images/5/59/ETH2014_piG0067_logic_bxb1_BUFFER-sfGFP.txt]]
[[File:ETH2014 piG0067 logic bxb1 BUFFER-sfGFP Map.png|link=http://2014.igem.org/wiki/images/5/59/ETH2014_piG0067_logic_bxb1_BUFFER-sfGFP.txt]]

Revision as of 20:25, 17 October 2014

iGEM ETH Zurich 2014