Team:ETH Zurich/lab/sequences

From 2014.igem.org

(Difference between revisions)
(Sensor Constructs)
(Sensor Constructs)
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[[File:ETH2014 piG0060 sensor prhlRstRBS-sfGFP Map.png|link=http://2014.igem.org/wiki/images/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt]]
[[File:ETH2014 piG0060 sensor prhlRstRBS-sfGFP Map.png|link=http://2014.igem.org/wiki/images/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt]]
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Expression of sfGFP is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The cis-repressive sequence inhibits the translation of sfGFP, since the RBS is blocked. The p15A is present at a copy number of approximately 15 to 25<sup>[[Team:ETH_Zurich/project/references#refChang|[35]]]</sup>.
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Expression of sfGFP is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The cis-repressive sequence inhibits the translation of sfGFP, since the RBS is blocked by secondary structures of the mRNA. The p15A is present at a copy number of approximately 15 to 25<sup>[[Team:ETH_Zurich/project/references#refChang|[35]]]</sup>.
[http://2014.igem.org/wiki/images/2/2c/PiG0062_sensor_pluxRcr12yRBS-sfGFP.txt piG0062]
[http://2014.igem.org/wiki/images/2/2c/PiG0062_sensor_pluxRcr12yRBS-sfGFP.txt piG0062]

Revision as of 19:47, 17 October 2014

iGEM ETH Zurich 2014