Team:ETH Zurich/lab/sequences

From 2014.igem.org

(Difference between revisions)
(Sensor Constructs)
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[http://2014.igem.org/wiki/images/f/f6/ETH2014_piG0040.txt piG0040]
[http://2014.igem.org/wiki/images/f/f6/ETH2014_piG0040.txt piG0040]
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LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 <sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20<sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
[[File:ETH2014_piG0040_Map.png|link=http://2014.igem.org/wiki/images/f/f6/ETH2014_piG0040.txt]]
[[File:ETH2014_piG0040_Map.png|link=http://2014.igem.org/wiki/images/f/f6/ETH2014_piG0040.txt]]
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[http://2014.igem.org/wiki/images/2/2c/ETH2014_piG0041.txt piG0041]
[http://2014.igem.org/wiki/images/2/2c/ETH2014_piG0041.txt piG0041]
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LuxR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 <sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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LuxR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20<sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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[http://2014.igem.org/wiki/images/d/db/ETH2014_piG0042.txt piG0042]
[http://2014.igem.org/wiki/images/d/db/ETH2014_piG0042.txt piG0042]
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RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 <sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20<sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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[http://2014.igem.org/wiki/images/c/ce/ETH2014_piG0042max.txt piG0042max]
[http://2014.igem.org/wiki/images/c/ce/ETH2014_piG0042max.txt piG0042max]
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RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR ([https://salis.psu.edu/software/forward RBS calculator]). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 <sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR ([https://salis.psu.edu/software/forward RBS calculator]). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20<sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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[http://2014.igem.org/wiki/images/1/18/ETH2014_piG0046.txt piG0046]
[http://2014.igem.org/wiki/images/1/18/ETH2014_piG0046.txt piG0046]
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LuxR is expressed under the weak constitutive promoter [http://parts.igem.org/Part:BBa_J23109 BBa_J23109] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 <sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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LuxR is expressed under the weak constitutive promoter [http://parts.igem.org/Part:BBa_J23109 BBa_J23109] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20<sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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[http://2014.igem.org/wiki/images/1/1b/ETH2014_piG0047.txt piG0047]
[http://2014.igem.org/wiki/images/1/1b/ETH2014_piG0047.txt piG0047]
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LuxR is expressed under the medium strong constitutive promoter [http://parts.igem.org/Part:BBa_J23111 BBa_J23111 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 <sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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LuxR is expressed under the medium strong constitutive promoter [http://parts.igem.org/Part:BBa_J23111 BBa_J23111 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20<sup>[[Team:ETH_Zurich/project/references#refBolivar|[15]]]</sup>.
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[http://2014.igem.org/wiki/images/4/49/ETH2014_piG0050.txt piG0050]
[http://2014.igem.org/wiki/images/4/49/ETH2014_piG0050.txt piG0050]
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
   
   
[[File:ETH2014_piG0050_Map.png|link=http://2014.igem.org/wiki/images/4/49/ETH2014_piG0050.txt]]
[[File:ETH2014_piG0050_Map.png|link=http://2014.igem.org/wiki/images/4/49/ETH2014_piG0050.txt]]
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[http://2014.igem.org/wiki/images/5/50/ETH2014_piG0050max.txt piG0050max]
[http://2014.igem.org/wiki/images/5/50/ETH2014_piG0050max.txt piG0050max]
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LuxI ([https://salis.psu.edu/software/forward RBS calculator]). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LuxI ([https://salis.psu.edu/software/forward RBS calculator]). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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[http://2014.igem.org/wiki/images/0/00/ETH2014_piG0051.txt piG0051]
[http://2014.igem.org/wiki/images/0/00/ETH2014_piG0051.txt piG0051]
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
   
   
[[File:ETH2014_piG0051_Map.png|link=http://2014.igem.org/wiki/images/0/00/ETH2014_piG0051.txt]]
[[File:ETH2014_piG0051_Map.png|link=http://2014.igem.org/wiki/images/0/00/ETH2014_piG0051.txt]]
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[http://2014.igem.org/wiki/images/9/94/ETH2014_piG0051max.txt piG0051max]
[http://2014.igem.org/wiki/images/9/94/ETH2014_piG0051max.txt piG0051max]
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for RhlI ([https://salis.psu.edu/software/forward RBS calculator]). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
+
RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for RhlI ([https://salis.psu.edu/software/forward RBS calculator]). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized<sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
[[File:ETH2014_piG0051max_Map.png|link=http://2014.igem.org/wiki/images/9/94/ETH2014_piG0051max.txt]]
[[File:ETH2014_piG0051max_Map.png|link=http://2014.igem.org/wiki/images/9/94/ETH2014_piG0051max.txt]]
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[http://2014.igem.org/wiki/images/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt piG0058]
[http://2014.igem.org/wiki/images/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt piG0058]
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Expression of sfGFP is induced when LasR bound to 3OC12-HSL bind to pLasR. The p15A is present at a copy number of approximately 15 to 25.
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Expression of sfGFP is induced when LasR bound to 3OC12-HSL bind to pLasR. The p15A is present at a copy number of approximately 15 to 25<sup>[[Team:ETH_Zurich/project/references#refChang|[35]]]</sup>.
[[File:ETH2014 piG0058 sensor plasRstRBS-sfGFP Map.png|link=http://2014.igem.org/wiki/images/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt]]
[[File:ETH2014 piG0058 sensor plasRstRBS-sfGFP Map.png|link=http://2014.igem.org/wiki/images/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt]]
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[http://2014.igem.org/wiki/images/0/0f/ETH2014_piG0059_sensor_pluxRstRBS-sfGFP.txt piG0059]
[http://2014.igem.org/wiki/images/0/0f/ETH2014_piG0059_sensor_pluxRstRBS-sfGFP.txt piG0059]
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Expression of sfGFP is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The p15A is present at a copy number of approximately 15 to 25.
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Expression of sfGFP is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The p15A is present at a copy number of approximately 15 to 25<sup>[[Team:ETH_Zurich/project/references#refChang|[35]]]</sup>.
[[File:ETH2014 piG0059 sensor pluxRstRBS-sfGFP Map.png|link=http://2014.igem.org/wiki/images/0/0f/ETH2014_piG0059_sensor_pluxRstRBS-sfGFP.txt]]
[[File:ETH2014 piG0059 sensor pluxRstRBS-sfGFP Map.png|link=http://2014.igem.org/wiki/images/0/0f/ETH2014_piG0059_sensor_pluxRstRBS-sfGFP.txt]]
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[http://2014.igem.org/wiki/images/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt piG0060]
[http://2014.igem.org/wiki/images/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt piG0060]
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Expression of sfGFP is induced when RhlR bound to 4C-HSL bind to pRhlR. The p15A is present at a copy number of approximately 15 to 25.
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Expression of sfGFP is induced when RhlR bound to 4C-HSL bind to pRhlR. The p15A is present at a copy number of approximately 15 to 25<sup>[[Team:ETH_Zurich/project/references#refChang|[35]]]</sup>.
[[File:ETH2014 piG0060 sensor prhlRstRBS-sfGFP Map.png|link=http://2014.igem.org/wiki/images/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt]]
[[File:ETH2014 piG0060 sensor prhlRstRBS-sfGFP Map.png|link=http://2014.igem.org/wiki/images/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt]]

Revision as of 19:36, 17 October 2014

iGEM ETH Zurich 2014