From 2014.igem.org

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Revision as of 19:28, 17 October 2014

iGEM ETH Zurich 2014

Sequences

The plasmid sequences can be accessed by clicking on the plasmid name (e.g. piG0040) or the plasmid picture.

Regulator Constructs

piG0040

LasR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 ^[15].

piG0041

LuxR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 ^[15].

piG0042

RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 ^[15].

piG0042max

RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlR (RBS calculator). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 ^[15].

piG0046

LuxR is expressed under the weak constitutive promoter BBa_J23109 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 ^[15].

piG0047

LuxR is expressed under the medium strong constitutive promoter BBa_J23111 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 ^[15].

Producer Constructs

piG0049

LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized ^[16].

piG0049max

LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LasI (RBS calculator). LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized ^[16].

piG0050

LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized ^[16].

piG0050max

LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LuxI (RBS calculator). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized ^[16].

piG0051

RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized ^[16].

piG0051max

RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlI (RBS calculator). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized ^[16].

Sensor Constructs

piG0058

Expression of sfGFP is induced when LasR bound to 3OC12-HSL bind to pLasR. The p15A is present at a copy number of approximately 15 to 25.

piG0059

Expression of sfGFP is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The p15A is present at a copy number of approximately 15 to 25.

piG0060

Expression of sfGFP is induced when RhlR bound to 4C-HSL bind to pRhlR. The p15A is present at a copy number of approximately 15 to 25.

BUFFER Gate Construct

piG0067

Signal Propagation Construct

piG0071

Combined Sensor and Producer Constructs

piG0096

piG0097

piG0099

@@ Line 105: / Line 105: @@
 [https://static.igem.org/mediawiki/2014/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt piG0058]
-Expression of sfGFP is induced when LasR bound to 3OC12-HSL bind to pLasR.
+Expression of sfGFP is induced when LasR bound to 3OC12-HSL bind to pLasR. The p15A is present at a copy number of approximately 15 to 25.
 [[File:ETH2014 piG0058 sensor plasRstRBS-sfGFP Map.png|link=https://static.igem.org/mediawiki/2014/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt]]
@@ Line 111: / Line 111: @@
 [https://static.igem.org/mediawiki/2014/0/0f/ETH2014_piG0059_sensor_pluxRstRBS-sfGFP.txt piG0059]
-Expression of sfGFP is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The p15A is a
+Expression of sfGFP is induced when LuxR bound to 3OC6-HSL bind to pLuxR. The p15A is present at a copy number of approximately 15 to 25.
 [[File:ETH2014 piG0059 sensor pluxRstRBS-sfGFP Map.png|link=https://static.igem.org/mediawiki/2014/0/0f/ETH2014_piG0059_sensor_pluxRstRBS-sfGFP.txt]]
@@ Line 117: / Line 117: @@
 [https://static.igem.org/mediawiki/2014/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt piG0060]
-Expression of sfGFP is induced when RhlR bound to 4C-HSL bind to pRhlR.
+Expression of sfGFP is induced when RhlR bound to 4C-HSL bind to pRhlR. The p15A is present at a copy number of approximately 15 to 25.
 [[File:ETH2014 piG0060 sensor prhlRstRBS-sfGFP Map.png|link=https://static.igem.org/mediawiki/2014/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt]]

Team:ETH Zurich/lab/sequences