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Team:ETH Zurich/lab/sequences
From 2014.igem.org
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[[File:ETH2014_piG0051max_Map.png|link=https://static.igem.org/mediawiki/2014/9/94/ETH2014_piG0051max.txt]] | [[File:ETH2014_piG0051max_Map.png|link=https://static.igem.org/mediawiki/2014/9/94/ETH2014_piG0051max.txt]] | ||
| - | == | + | [https://static.igem.org/mediawiki/2014/9/95/ETH2014_piG0109_pluxRRR12y-sfGFP_EcoRI-_XbaI-.txt piG0109] |
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| + | [[File:ETH2014 piG0109 pluxRRR12y-sfGFP EcoRI- XbaI- Map.png|link=https://static.igem.org/mediawiki/2014/9/95/ETH2014_piG0109_pluxRRR12y-sfGFP_EcoRI-_XbaI-.txt]] | ||
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| + | [https://static.igem.org/mediawiki/2014/f/f9/ETH2014_piG0110_prhlRRR12-sfGFP_EcoRI-_XbaI-.txt piG0110] | ||
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| + | [[File:ETH2014 piG0110 prhlRRR12-sfGFP EcoRI- XbaI- Map.png|link=https://static.igem.org/mediawiki/2014/f/f9/ETH2014_piG0110_prhlRRR12-sfGFP_EcoRI-_XbaI-.txt]] | ||
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| + | ==Sensor Constructs== | ||
[https://static.igem.org/mediawiki/2014/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt piG0058] | [https://static.igem.org/mediawiki/2014/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt piG0058] | ||
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[[File:ETH2014 piG0066 sensor prhlRRR12-sfGFP Map.png|link=https://static.igem.org/mediawiki/2014/2/24/ETH2014_piG0066_sensor_prhlRRR12-sfGFP.txt]] | [[File:ETH2014 piG0066 sensor prhlRRR12-sfGFP Map.png|link=https://static.igem.org/mediawiki/2014/2/24/ETH2014_piG0066_sensor_prhlRRR12-sfGFP.txt]] | ||
| + | ==BUFFER Gate Construct== | ||
[https://static.igem.org/mediawiki/2014/5/59/ETH2014_piG0067_logic_bxb1_BUFFER-sfGFP.txt piG0067] | [https://static.igem.org/mediawiki/2014/5/59/ETH2014_piG0067_logic_bxb1_BUFFER-sfGFP.txt piG0067] | ||
[[File:ETH2014 piG0067 logic bxb1 BUFFER-sfGFP Map.png|link=https://static.igem.org/mediawiki/2014/5/59/ETH2014_piG0067_logic_bxb1_BUFFER-sfGFP.txt]] | [[File:ETH2014 piG0067 logic bxb1 BUFFER-sfGFP Map.png|link=https://static.igem.org/mediawiki/2014/5/59/ETH2014_piG0067_logic_bxb1_BUFFER-sfGFP.txt]] | ||
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| + | ==Signal Propagation Constructs== | ||
[https://static.igem.org/mediawiki/2014/1/1d/ETH2014_piG0071_sensor_pluxRRR12y_bxb1_mCherry.txt piG0071] | [https://static.igem.org/mediawiki/2014/1/1d/ETH2014_piG0071_sensor_pluxRRR12y_bxb1_mCherry.txt piG0071] | ||
[[File:ETH2014 piG0071 sensor pluxRRR12y bxb1 mCherry Map.png|link=https://static.igem.org/mediawiki/2014/1/1d/ETH2014_piG0071_sensor_pluxRRR12y_bxb1_mCherry.txt]] | [[File:ETH2014 piG0071 sensor pluxRRR12y bxb1 mCherry Map.png|link=https://static.igem.org/mediawiki/2014/1/1d/ETH2014_piG0071_sensor_pluxRRR12y_bxb1_mCherry.txt]] | ||
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| + | ==Combined Sensor and Producer Constructs== | ||
[https://static.igem.org/mediawiki/2014/f/f5/ETH2014_piG0096_pluxRRR12y-sfGFP-maxRBS-luxI.txt piG0096] | [https://static.igem.org/mediawiki/2014/f/f5/ETH2014_piG0096_pluxRRR12y-sfGFP-maxRBS-luxI.txt piG0096] | ||
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[[File:ETH2014_piG0099_pluxstRBS-sfGFP-stRBS-luxI_Map.png|link=https://static.igem.org/mediawiki/2014/6/69/ETH2014_piG0099_pluxstRBS-sfGFP-stRBS-luxI.txt]] | [[File:ETH2014_piG0099_pluxstRBS-sfGFP-stRBS-luxI_Map.png|link=https://static.igem.org/mediawiki/2014/6/69/ETH2014_piG0099_pluxstRBS-sfGFP-stRBS-luxI.txt]] | ||
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Revision as of 18:59, 17 October 2014
Sequences
The plasmid sequences can be accessed by clicking on the plasmid name (e.g. piG0040) or the plasmid picture.
Regulator Constructs
LasR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlR (RBS calculator). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the weak constitutive promoter BBa_J23109 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the medium strong constitutive promoter BBa_J23111 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
Producer Constructs
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LasI (RBS calculator). LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LuxI (RBS calculator). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlI (RBS calculator). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
Sensor Constructs
BUFFER Gate Construct
Signal Propagation Constructs
Combined Sensor and Producer Constructs
