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Team:ETH Zurich/lab/sequences
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[[File:ETH2014 piG0058 sensor plasRstRBS-sfGFP Map.png|https://static.igem.org/mediawiki/2014/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt]] | [[File:ETH2014 piG0058 sensor plasRstRBS-sfGFP Map.png|https://static.igem.org/mediawiki/2014/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt]] | ||
| + | [https://static.igem.org/mediawiki/2014/2/2c/PiG0062_sensor_pluxRcr12yRBS-sfGFP.txt piG0062] | ||
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| + | [[File:ETH2014 piG0062 sensor pluxRcr12yRBS-sfGFP Map.png|https://static.igem.org/mediawiki/2014/2/2c/PiG0062_sensor_pluxRcr12yRBS-sfGFP.txt]] | ||
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| + | [https://static.igem.org/mediawiki/2014/2/24/ETH2014_piG0066_sensor_prhlRRR12-sfGFP.txt piG0066] | ||
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| + | [[File:ETH2014 piG0066 sensor prhlRRR12-sfGFP Map.png|https://static.igem.org/mediawiki/2014/2/24/ETH2014_piG0066_sensor_prhlRRR12-sfGFP.txt]] | ||
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| + | [https://static.igem.org/mediawiki/2014/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt piG0060]] | ||
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| + | [[File:ETH2014 piG0060 sensor prhlRstRBS-sfGFP Map.png|https://static.igem.org/mediawiki/2014/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt]] | ||
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| + | [https://static.igem.org/mediawiki/2014/d/da/ETH2014_piG0065_sensor_pluxRRR12y-sfGFP.txt piG0065] | ||
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| + | [[File:ETH2014 piG0065 sensor pluxRRR12y-sfGFP Map.png|https://static.igem.org/mediawiki/2014/d/da/ETH2014_piG0065_sensor_pluxRRR12y-sfGFP.txt]] | ||
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Revision as of 18:41, 17 October 2014
Sequences
The plasmid sequences can be accessed by clicking on the plasmid name (e.g. piG0040)
Regulator Constructs
LasR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlR (RBS calculator). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the weak constitutive promoter BBa_J23109 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the medium strong constitutive promoter BBa_J23111 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
Producer Constructs
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LasI (RBS calculator). LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LuxI (RBS calculator). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlI (RBS calculator). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
Provisorisch
