Team:ETH Zurich/lab/sequences

From 2014.igem.org

(Difference between revisions)
(Producer Constructs)
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[https://static.igem.org/mediawiki/2014/4/43/ETH2014_piG0049.txt piG0049]
[https://static.igem.org/mediawiki/2014/4/43/ETH2014_piG0049.txt piG0049]
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LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is not well characterized; ts copy number is reported to be approximately 5 <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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[https://static.igem.org/mediawiki/2014/b/bc/ETH2014_piG0049max.txt piG0049max]
[https://static.igem.org/mediawiki/2014/b/bc/ETH2014_piG0049max.txt piG0049max]
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LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LasI ([https://salis.psu.edu/software/forward RBS calculator]). LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LasI ([https://salis.psu.edu/software/forward RBS calculator]). LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5 <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
   
   
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[https://static.igem.org/mediawiki/2014/4/49/ETH2014_piG0050.txt piG0050]
[https://static.igem.org/mediawiki/2014/4/49/ETH2014_piG0050.txt piG0050]
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5 <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
   
   
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[https://static.igem.org/mediawiki/2014/5/50/ETH2014_piG0050max.txt piG0050max]
[https://static.igem.org/mediawiki/2014/5/50/ETH2014_piG0050max.txt piG0050max]
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LuxI ([https://salis.psu.edu/software/forward RBS calculator]). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LuxI ([https://salis.psu.edu/software/forward RBS calculator]). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5 <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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[https://static.igem.org/mediawiki/2014/0/00/ETH2014_piG0051.txt piG0051]
[https://static.igem.org/mediawiki/2014/0/00/ETH2014_piG0051.txt piG0051]
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5 <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
   
   
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[https://static.igem.org/mediawiki/2014/9/94/ETH2014_piG0051max.txt piG0051max]
[https://static.igem.org/mediawiki/2014/9/94/ETH2014_piG0051max.txt piG0051max]
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for RhlI ([https://salis.psu.edu/software/forward RBS calculator]). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.
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RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for RhlI ([https://salis.psu.edu/software/forward RBS calculator]). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5 <sup>[[Team:ETH_Zurich/project/references#refLennen|[16]]]</sup>.

Revision as of 15:06, 12 October 2014

iGEM ETH Zurich 2014