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Team:ETH Zurich/lab/sequences
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LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. | LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. | ||
| - | [[File:ETH2014_piG0040_Map.png | + | [[File:ETH2014_piG0040_Map.png]] |
Revision as of 21:12, 11 October 2014
Sequences
Regulator Constructs
LasR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR.
LuxR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlR (RBS calculator). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
LuxR is expressed under the weak constitutive promoter BBa_J23109 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
LuxR is expressed under the medium strong constitutive promoter BBa_J23111 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
Producer Constructs
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasI produces the quorum sensing molecule 3OC12-HSL.
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LasI (RBS calculator). LasI produces the quorum sensing molecule 3OC12-HSL.
LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxI produces the quorum sensing molecule 3OC6-HSL.
LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LuxI (RBS calculator). LuxI produces the quorum sensing molecule 3OC6-HSL.
RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlI produces the quorum sensing molecule C4-HSL.
RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlI (RBS calculator). RhlI produces the quorum sensing molecule C4-HSL.
