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Team:ETH Zurich/lab/sequences
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LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. | LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. | ||
| - | + | [[File:ETH2014_piG0040_Map.png]] | |
[https://static.igem.org/mediawiki/2014/2/2c/ETH2014_piG0041.txt piG0041] | [https://static.igem.org/mediawiki/2014/2/2c/ETH2014_piG0041.txt piG0041] | ||
LuxR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. | LuxR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. | ||
| - | + | [[File:ETH2014_piG0041_Map.png]] | |
[https://static.igem.org/mediawiki/2014/d/db/ETH2014_piG0042.txt piG0042] | [https://static.igem.org/mediawiki/2014/d/db/ETH2014_piG0042.txt piG0042] | ||
RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | ||
| - | + | [[File:ETH2014_piG0042_Map.png]] | |
[https://static.igem.org/mediawiki/2014/c/ce/ETH2014_piG0042max.txt piG0042max] | [https://static.igem.org/mediawiki/2014/c/ce/ETH2014_piG0042max.txt piG0042max] | ||
RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR ([https://salis.psu.edu/software/forward RBS calculator]). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR ([https://salis.psu.edu/software/forward RBS calculator]). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | ||
| - | + | [[File:ETH2014_piG0042max_Map.png]] | |
[https://static.igem.org/mediawiki/2014/1/18/ETH2014_piG0046.txt piG0046] | [https://static.igem.org/mediawiki/2014/1/18/ETH2014_piG0046.txt piG0046] | ||
| - | LuxR is expressed under the weak constitutive promoter [http://parts.igem.org/Part:BBa_J23109 BBa_J23109] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. | + | LuxR is expressed under the weak constitutive promoter [http://parts.igem.org/Part:BBa_J23109 BBa_J23109] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. |
| + | [[File:ETH2014_piG0046_Map.png]] | ||
[https://static.igem.org/mediawiki/2014/1/1b/ETH2014_piG0047.txt piG0047] | [https://static.igem.org/mediawiki/2014/1/1b/ETH2014_piG0047.txt piG0047] | ||
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LuxR is expressed under the medium strong constitutive promoter [http://parts.igem.org/Part:BBa_J23111 BBa_J23111 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. | LuxR is expressed under the medium strong constitutive promoter [http://parts.igem.org/Part:BBa_J23111 BBa_J23111 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. | ||
[[File:ETH2014_piG0047_Map.png]] | [[File:ETH2014_piG0047_Map.png]] | ||
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==Producer Constructs== | ==Producer Constructs== | ||
Revision as of 21:09, 11 October 2014
Sequences
Regulator Constructs
LasR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR.
LuxR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
RhlR is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlR (RBS calculator). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
LuxR is expressed under the weak constitutive promoter BBa_J23109 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
LuxR is expressed under the medium strong constitutive promoter BBa_J23111 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
Producer Constructs
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LasI produces the quorum sensing molecule 3OC12-HSL.
LasI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LasI (RBS calculator). LasI produces the quorum sensing molecule 3OC12-HSL.
LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxI produces the quorum sensing molecule 3OC6-HSL.
LuxI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for LuxI (RBS calculator). LuxI produces the quorum sensing molecule 3OC6-HSL.
RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlI produces the quorum sensing molecule C4-HSL.
RhlI is expressed under the strong constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlI (RBS calculator). RhlI produces the quorum sensing molecule C4-HSL.
