Team:ETH Zurich/lab/sequences
From 2014.igem.org
(Difference between revisions)
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[https://static.igem.org/mediawiki/2014/c/ce/ETH2014_piG0042max.txt piG0042max] | [https://static.igem.org/mediawiki/2014/c/ce/ETH2014_piG0042max.txt piG0042max] | ||
- | RhlR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. | + | RhlR is expressed under the constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. |
[https://static.igem.org/mediawiki/2014/9/93/ETH2014_piG0043.txt piG0043] | [https://static.igem.org/mediawiki/2014/9/93/ETH2014_piG0043.txt piG0043] |
Revision as of 16:16, 10 October 2014
Sequences
Regulator Constructs
piG0041 LuxR is expressed under the constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR.
piG0042 RhlR is expressed under the constitutive promoter BBa_J23100 controlled by the RBS BBa_B0034. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
piG0042max RhlR is expressed under the constitutive promoter BBa_J23100 controlled by an RBS optimised for RhlR. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR.
Producer Constructs