Team:ETH Zurich/lab/protocols

From 2014.igem.org

(Difference between revisions)
(Restriction Endonuclease Reactions)
(Restriction Endonuclease Reactions)
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{| border="0" class="wikitable"
{| border="0" class="wikitable"
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! Double digestion  
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! Double digestion  
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| align="left" width="40%" | 1-2.5 μL restriction endonuclease 1
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| align="left" | 1-2.5 μL restriction endonuclease 1
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|-
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| align="left" width="40%" | 1-2.5 μL restriction endonuclease 2  
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| align="left" | 1-2.5 μL restriction endonuclease 2  
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|-
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| align="left" width="40%" | 5 μL Cut Smart Buffer  
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| align="left" | 5 μL Cut Smart Buffer  
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| align="left" width="40%" | 1-3 μg template DNA
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| align="left" | 1-3 μg template DNA
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| align="left" width="40%" | add H<sub>2</sub>O to reach a total volume of 50 μL
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| align="left" | add H<sub>2</sub>O to reach a total volume of 50 μL
|}
|}

Revision as of 18:49, 13 August 2014

iGEM ETH Zurich 2014

Retrieved from "http://2014.igem.org/Team:ETH_Zurich/lab/protocols"