Team:ETH Zurich/lab/protocols

From 2014.igem.org

(Difference between revisions)
(Materials)
(Materials)
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| align="left" | Tris(hydroxymethyl)aminomethane || align="left" | 1.21 g
| align="left" | Tris(hydroxymethyl)aminomethane || align="left" | 1.21 g
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| align="left" | ''Fill up to 1000 ml with H<sub>2</sub>O'' ||  
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| align="left" | ''Fill up to 980 ml with H<sub>2</sub>O'' ||  
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| align="left" | ''Adjust pH to 7  with HCl'' ||  
| align="left" | ''Adjust pH to 7  with HCl'' ||  
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| align="left" | CaCl<sub>2</sub>&sdot;H<sub>2</sub>O || align="left" | 1 g
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| align="left" | CaCl<sub>2</sub>&sdot;H<sub>2</sub>O (100 mM) || align="left" | 20 mL
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| align="left" | ''Sterilize by filtration'' ||
| align="left" | ''Sterilize by filtration'' ||
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According to [http://www.qiagen.com/resources/molecular-biology-methods/dna/ QIAGEN ''DNA protocols and applications'']
 
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Revision as of 12:26, 14 October 2014

iGEM ETH Zurich 2014