Team:ETH Zurich/lab/protocols

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(Difference between revisions)
(Methods)
(Preparation of electrocompetent E. coli)
Line 202: Line 202:
# Centrifuge for 6 min
# Centrifuge for 6 min
# Repeat washing step followed by centrifugation 3 times
# Repeat washing step followed by centrifugation 3 times
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# After last centrifugation step, discard supernatant and resuspend pellet in 50uL chilled water.  
+
# After last centrifugation step, discard supernatant and resuspend pellet in 50uL chilled water.
 +
# Add plasmid you want to transform
# Electroporate
# Electroporate
# Add SOC medium and let cells recover at 37°C for 1 hour
# Add SOC medium and let cells recover at 37°C for 1 hour

Revision as of 10:35, 11 October 2014

iGEM ETH Zurich 2014