Team:ETH Zurich/lab/protocols

From 2014.igem.org

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http://www.nanodrop.com/Productnd2000overview.aspx
http://www.nanodrop.com/Productnd2000overview.aspx
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===Tecan Infinite M200 Pro™ and the Tecan iControl™ software===
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For performing fluorescence measurements we used a Tecan Infinite M200 ProTM equipped with the Tecan iControl TM software.
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The Infinite 200 PRO is a user-friendly and affordable multimode reader, designed to cater for the needs of today’s applications. Based on the highly successful Infinite 200 series of microplate reader, the Infinite 200 PRO can provide a full range of leading detection methods in one easy-to-use modular instrument, with either Quad4 monochromator™ or filter-based technologies. Users can select the desired modules to create the perfect reader for their needs, with the option to upgrade as requirements change. The Infinite 200 PRO offers excellent sensitivity, multiplexing capabilities and high format flexibility, including 6- to 384-well microplates, PCR plates, cuvettes and Tecan’s patentedNanoQuant Plate™ for low sample volumes.
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*For uncompromised performance in all detection modes, the Infinite 200 PRO uses advanced optics and high-performance detectors, optimized for the requirements of fluorescence, luminescence and absorbance reading. The Infinite 200 PRO offers a wide range of detection modes, including:
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*Fluorescence intensity (top and bottom reading) (UV – NIR & wavelength scanning)
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*Fluorescence resonance energy transfer (FRET)
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*Time resolved fluorescence energy transfer (TR-FRET) like HTRF®
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*Time resolved fluorescence (TRF)
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*Fluorescence polarization (FP)
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*Flash luminescence
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*Glow luminescence
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*Dual-color luminescence (including BRET 1 & BRET 2 applications)
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*Absorbance  (UV – NIR & wavelength scanning)
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*AlphaScreen® and AlphaLISA® technology
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*Injectors system for up to two reagents
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*Temperature control
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*Small sample measurement in NanoQuant Plate
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http://www.tecan.com/platform/apps/product/index.asp?MenuID=1812&ID=1916&Menu=1&Item=21.2.10.1
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Revision as of 12:53, 10 October 2014

iGEM ETH Zurich 2014