Team:ETH Zurich/lab/protocols

From 2014.igem.org

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(LB medium)
(LB medium)
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===LB medium===
===LB medium===
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*Suspend/dissolve the powder in 1 L of purified water:
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Suspend/dissolve the powder in 1 L of purified water:
Difco™ LB Agar, Miller – 40 g;
Difco™ LB Agar, Miller – 40 g;
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Mix thoroughly.
Mix thoroughly.
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*Heat the agar medium with frequent agitation and boil for 1 minute to completely dissolve the powder.
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Heat the agar medium with frequent agitation and boil for 1 minute to completely dissolve the powder.
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*Autoclave at 121°C for 15 minutes.
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Autoclave at 121°C for 15 minutes.
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*Test samples of the finished product for performance using stable, typical control cultures.
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Test samples of the finished product for performance using stable, typical control cultures.
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Check LB Agar, Miller • LB Broth, Miller for more information.
 
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https://www.bd.com/europe/regulatory/Assets/IFU/Difco_BBL/244510.pdf
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According to [https://www.bd.com/europe/regulatory/Assets/IFU/Difco_BBL/244510.pdf LB Agar, Miller; LB Broth, Miller]
===Preparation of antibiotics stock (1000x)===
===Preparation of antibiotics stock (1000x)===

Revision as of 15:48, 10 October 2014

iGEM ETH Zurich 2014