Team:ETH Zurich/lab/protocols

From 2014.igem.org

(Difference between revisions)
(Complex bead medium (CB medium))
(Methods)
Line 298: Line 298:
|}
|}
 +
===Defined M9 medium with 0.4 % glycerol===
 +
 +
 +
Suspend/dissolve the sterile compounds in purified water to give 1L:
 +
 +
{| border="0"
 +
|-
 +
| align="left"  width="300"| [http://openwetware.org/wiki/M9_salts M9 salts, 5x] || align="left" | 200 mL
 +
|-
 +
| align="left" | 30% (v/v) Glycerol || align="left" | 13.33 mL
 +
|-
 +
| align="left" | Thiamin (10 g/L) || align="left" |  2 mL
 +
|-
 +
| align="left" | TE-US solution || align="left" | 1 mL
 +
|-
 +
| align="left" | 1M CaCl<sub>2</sub> || align="left" | 0.5 mL
 +
|-
 +
| align="left" | 1M MgSO<sub>4</sub> || align="left" | 2 mL
 +
|}
 +
 +
<br/>
 +
<br/>
===SOC===
===SOC===

Revision as of 13:56, 17 October 2014

iGEM ETH Zurich 2014