Team:ETH Zurich/lab/chip

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(Time-Lapse Movies)
(Time-Lapse Movies)
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==Time-Lapse Movies==
==Time-Lapse Movies==
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Below you find an overview of the time-lapse movies taken during the summer. In the very first trial the wells were filled with LB agar, holes were punched with a pipette tip and filled with highlighter-ink (pyranine) to visualize diffusion. Later, different set-ups were tested: chambers filled with liquid medium separated by solidified 2% agarose in the connecting channel and alginate beads in liquid medium. We continued with the 'alginate beads in  liquid medium' set-up, as it yielded the most promising intermediate results, and could then finally show cell-to-cell communication of bacteria confined in beads on our millifluid chip.
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Below you find an overview of the time-lapse movies taken during the summer. In the very first trial the wells were filled with LB agar, holes were punched with a pipette tip and filled with highlighter-ink (pyranine) to visualize diffusion (see video 1). Later, different set-ups were tested: chambers filled with liquid medium separated by solidified 2% agarose in the connecting channel and alginate beads in liquid medium. We continued with the 'alginate beads in  liquid medium' set-up, as it yielded the most promising intermediate results, and could then finally show cell-to-cell communication of bacteria confined in beads on our millifluid chip.
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|{{:Team:ETH_Zurich/Templates/Video|width=300px|id=video2|ratio=143/100|srcMP4=<html>https://static.igem.org/mediawiki/2014/1/18/ETH_Zurich_2014_two_wells_liquid_culture_small.mp4</html>}}
|{{:Team:ETH_Zurich/Templates/Video|width=300px|id=video2|ratio=143/100|srcMP4=<html>https://static.igem.org/mediawiki/2014/1/18/ETH_Zurich_2014_two_wells_liquid_culture_small.mp4</html>}}
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|'''Video 1''' text1
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|'''Video 1 The very first diffusion experiment with highlighter ink (pyranine).''' The wells of the PDMS chip are filled with LB agar. About 2 μL ink were added in a punched whole on one side of the two wells. ~4500x faster than real-time.
|'''Video 2''' text2
|'''Video 2''' text2
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Revision as of 00:02, 18 October 2014

iGEM ETH Zurich 2014