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Team:ETH Zurich/lab/biobrick/used1
From 2014.igem.org
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=== First Level crosstalk: LuxR binds to different HSL and activates the promoter === | === First Level crosstalk: LuxR binds to different HSL and activates the promoter === | ||
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| + | [[File:ETH Zurich 1crosstalkPlux.png|400px|center]] | ||
=== Second Level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter === | === Second Level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter === | ||
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| + | [[File:ETH Zurich 2crosstalkPlux.png|400px|center]] | ||
== Second order crosstalk: Combination of both cross-talk levels == | == Second order crosstalk: Combination of both cross-talk levels == | ||
Other regulatory proteins, like LasR, bind to different HSL and activates the promoter | Other regulatory proteins, like LasR, bind to different HSL and activates the promoter | ||
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| + | [[File:ETH Zurich 3crosstalkPlux.png|400px|center]] | ||
== Results == | == Results == | ||
Revision as of 12:10, 16 October 2014
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<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014 |
Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSLBackground informationSystems consideredModeling leakinessResultsCharacterization of two-level crosstalk on the promoterBackground informationSystem consideredModeling crosstalkFirst-order crosstalkFirst Level crosstalk: LuxR binds to different HSL and activates the promoter Second Level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter Second order crosstalk: Combination of both cross-talk levelsOther regulatory proteins, like LasR, bind to different HSL and activates the promoter  ResultsCharacterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentrationBackground informationSystems consideredModeling promoter's sensitivityResults |
