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Team:ETH Zurich/lab/biobrick/used1
From 2014.igem.org
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| - | + | == Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentration == | |
| + | |||
| + | == Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL == | ||
| + | |||
| + | === System considered === | ||
| + | |||
| + | === Modeling leakiness ==== | ||
| + | |||
| + | === Results === | ||
| + | |||
| + | == Characterization of two-level crosstalk on the promoter == | ||
| + | |||
| + | === Systems considered === | ||
| + | |||
| + | === Modeling crosstalk === | ||
| + | |||
| + | === First-level crosstalk: LuxR binds to different HSL and activates the promoter === | ||
| + | |||
| + | === Second-level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter === | ||
| + | |||
| + | === Combination of both levels: other regulatory proteins, like LasR, bind to different HSL and activates the promoter === | ||
| + | |||
| + | === Results === | ||
|}; | |}; | ||
Revision as of 12:30, 3 October 2014
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<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014 |
Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentrationCharacterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSLSystem consideredModeling leakiness =ResultsCharacterization of two-level crosstalk on the promoterSystems consideredModeling crosstalkFirst-level crosstalk: LuxR binds to different HSL and activates the promoterSecond-level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoterCombination of both levels: other regulatory proteins, like LasR, bind to different HSL and activates the promoterResults |
