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Team:ETH Zurich/lab/biobrick/used1
From 2014.igem.org
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= Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL = | = Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL = | ||
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| + | The amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system was shown to influence the pLuxR promoter's basal expression or leakiness. By using the three different constitutive promoters [http://parts.igem.org/Part:BBa_J23100:Experience BBa_J23100], [http://parts.igem.org/Part:BBa_J23109:Experience BBa_J23109], and [http://parts.igem.org/Part:BBa_J23111:Experience BBa_J23111] for the production of LuxR we could measure this effect in terms of fluorescence. | ||
== Background information == | == Background information == | ||
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| + | We used an ''E. coli'' TOP10 strain | ||
=== Systems considered === | === Systems considered === | ||
Revision as of 14:39, 16 October 2014
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<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014 |
Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSLThe amount of regulator LuxR (BBa_C0062) in the system was shown to influence the pLuxR promoter's basal expression or leakiness. By using the three different constitutive promoters BBa_J23100, BBa_J23109, and BBa_J23111 for the production of LuxR we could measure this effect in terms of fluorescence. Background informationWe used an E. coli TOP10 strain Systems consideredModeling leakinessResultsCharacterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentrationBackground informationSystems consideredModeling promoter's sensitivityResultsCharacterization of two-level crosstalk on the promoterBackground informationSystem consideredModeling crosstalkFirst-order crosstalkFirst Level crosstalk: LuxR binds to different HSL and activates the promoter Second Level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter Second order crosstalk: Combination of both cross-talk levelsOther regulatory proteins, like LasR, bind to different HSL and activates the promoter  Results |
