Team:ETH Zurich/lab/bead

From 2014.igem.org

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(Production)
(Production)
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|Bacteria are resuspended and diluted in NaCl-solution (0.9 % in H<sub>2</sub>O) so as to achieve the desired cell density. The resuspension was added to alginate (2.5%) to reach a alginate concentration of 2%. The viscous solution is filled into a syringe containing an appropriate needle device. Droplets should fall form a height of approximately 30 cm into a CaCl<sub>2</sub> solution.
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|Bacteria are resuspended and diluted in NaCl-solution (0.9 % in H<sub>2</sub>O) so as to achieve the desired cell density. The resuspension was added to alginate (2.5%) to reach a alginate concentration of 2%. The viscous solution is filled into a syringe containing an appropriate needle device and extruded at a constant, slow velocity. Ideally, the droplets should fall form a height of approximately 30 cm into a CaCl<sub>2</sub> solution.
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|[[File:ETH2014_BeadsProduction.jpg|300px]]
|[[File:ETH2014_BeadsProduction.jpg|300px]]

Revision as of 16:17, 15 October 2014

iGEM ETH Zurich 2014