http://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&feed=atom&action=historyTeam:ETH Zurich/expresults/qs/tab-prhl - Revision history2024-03-28T12:57:31ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=364484&oldid=prevLnadine at 22:54, 17 October 20142014-10-17T22:54:20Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:left|In this set of experiments the promoter pRhl <del class="diffchange diffchange-inline">(ref) </del>was tested for potential crosstalk. In the top left position we observe the induction of pRhl <del class="diffchange diffchange-inline">(ref)</del>by C4-HSL bound to the regulator RhlR<del class="diffchange diffchange-inline">(ref)</del>. The switching behaviour was observed at a C4-HSL concentration of 1 μM. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:left|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR <del class="diffchange diffchange-inline">(ref)</del>regulator and subsequently the promoter pRhl <del class="diffchange diffchange-inline">(ref)</del>insignificant crosstalk has been observed.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In this set of experiments the promoter <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0071 </ins>pRhl<ins class="diffchange diffchange-inline">] </ins>was tested for potential crosstalk. In the top left position we observe the induction of <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0071 </ins>pRhl<ins class="diffchange diffchange-inline">] </ins>by C4-HSL bound to the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0171 </ins>regulator RhlR<ins class="diffchange diffchange-inline">]</ins>. The switching behaviour was observed at a C4-HSL concentration of 1 μM. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Severe crosstalk was observed in the case of 3OC6-HSL binding the RhlR <del class="diffchange diffchange-inline">(reg)</del>regulator followed by induction of pRhl<del class="diffchange diffchange-inline">(reg)</del>. The transition occurred at a concentration of the inducer molecule of 1 μM but compared to the reference curve a lower <del class="diffchange diffchange-inline"> </del>value of fluorescence per OD was observed (1000 a.u.). Another case of crosstalk with the pRhl <del class="diffchange diffchange-inline">(ref) </del>was detected with 3OC12-HSL binding to the corresponding LasR <del class="diffchange diffchange-inline">(ref)</del>regulator followed by inducing the promoter pRhl<del class="diffchange diffchange-inline">(ref)</del>. Here switching occurred at a concentration 1 nM of 3OC12-HSL and reached fluorescence per OD of 750 a.u.. This is approximately 0.5 fold the value of the fluorescence per OD shown by the reference curve indicated in green.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0171 </ins>RhlR regulator<ins class="diffchange diffchange-inline">] </ins>and subsequently the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0071 </ins>promoter pRhl<ins class="diffchange diffchange-inline">]</ins>insignificant crosstalk has been observed.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Severe crosstalk was observed in the case of 3OC6-HSL binding the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0171 </ins>RhlR regulator<ins class="diffchange diffchange-inline">] </ins>followed by induction of <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0071 </ins>pRhl<ins class="diffchange diffchange-inline">]</ins>. The transition occurred at a concentration of the inducer molecule of 1 μM but compared to the reference curve a lower value of fluorescence per OD was observed (1000 a.u.). Another case of crosstalk with the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0071 </ins>pRhl<ins class="diffchange diffchange-inline">] </ins>was detected with 3OC12-HSL binding to the corresponding <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0179 </ins>LasR regulator<ins class="diffchange diffchange-inline">] </ins>followed by inducing the promoter <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0071 </ins>pRhl<ins class="diffchange diffchange-inline">]</ins>. Here switching occurred at a concentration 1 nM of 3OC12-HSL and reached fluorescence per OD of 750 a.u.. This is approximately 0.5 fold the value of the fluorescence per OD shown by the reference curve indicated in green.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
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</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=353347&oldid=prevLnadine at 20:57, 17 October 20142014-10-17T20:57:32Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:left|In this set of <del class="diffchange diffchange-inline">experiment </del>the promoter pRhl was tested for <del class="diffchange diffchange-inline">possible </del>crosstalk. In the top <del class="diffchange diffchange-inline">row </del>left <del class="diffchange diffchange-inline">graph </del>we <del class="diffchange diffchange-inline">see the </del>the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of <del class="diffchange diffchange-inline">the inducer molecule of 10<sup>3</sup> nM</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:left|In this set of <ins class="diffchange diffchange-inline">experiments </ins>the promoter pRhl <ins class="diffchange diffchange-inline">(ref) </ins>was tested for <ins class="diffchange diffchange-inline">potential </ins>crosstalk. In the top left <ins class="diffchange diffchange-inline">position </ins>we <ins class="diffchange diffchange-inline">observe </ins>the induction of pRhl <ins class="diffchange diffchange-inline">(ref)</ins>by C4-HSL bound to <ins class="diffchange diffchange-inline">the </ins>regulator RhlR<ins class="diffchange diffchange-inline">(ref)</ins>. The switching behaviour was observed at a <ins class="diffchange diffchange-inline">C4-HSL </ins>concentration of <ins class="diffchange diffchange-inline">1 μM</ins>. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl <del class="diffchange diffchange-inline">negligible </del>crosstalk has been observed.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR <ins class="diffchange diffchange-inline">(ref)</ins>regulator and subsequently the promoter pRhl <ins class="diffchange diffchange-inline">(ref)insignificant </ins>crosstalk has been observed.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Severe crosstalk was <del class="diffchange diffchange-inline">detected </del>in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of <del class="diffchange diffchange-inline">10<sup>3</sup> nM </del>but compared to the reference curve a lower <del class="diffchange diffchange-inline">(1000 AU) </del>value of fluorescence was observed. <del class="diffchange diffchange-inline">The second </del>case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. <del class="diffchange diffchange-inline">There the </del>switching occurred at a concentration <del class="diffchange diffchange-inline">1nM </del>of 3OC12-HSL <del class="diffchange diffchange-inline">reaching a </del>fluorescence of 750 <del class="diffchange diffchange-inline">AU</del>. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Severe crosstalk was <ins class="diffchange diffchange-inline">observed </ins>in the case of 3OC6-HSL binding the RhlR <ins class="diffchange diffchange-inline">(reg)</ins>regulator followed by induction of pRhl<ins class="diffchange diffchange-inline">(reg)</ins>. The transition occurred at a concentration of the inducer molecule of <ins class="diffchange diffchange-inline">1 μM </ins>but compared to the reference curve a lower <ins class="diffchange diffchange-inline"> </ins>value of fluorescence <ins class="diffchange diffchange-inline">per OD </ins>was observed <ins class="diffchange diffchange-inline">(1000 a</ins>.<ins class="diffchange diffchange-inline">u.). Another </ins>case of crosstalk with the pRhl <ins class="diffchange diffchange-inline">(ref) </ins>was detected with 3OC12-HSL binding to the corresponding LasR <ins class="diffchange diffchange-inline">(ref)</ins>regulator followed by inducing the promoter pRhl<ins class="diffchange diffchange-inline">(ref)</ins>. <ins class="diffchange diffchange-inline">Here </ins>switching occurred at a concentration <ins class="diffchange diffchange-inline">1 nM </ins>of 3OC12-HSL <ins class="diffchange diffchange-inline">and reached </ins>fluorescence <ins class="diffchange diffchange-inline">per OD </ins>of 750 <ins class="diffchange diffchange-inline">a.u.</ins>. This is approximately 0.5 fold the value of the fluorescence <ins class="diffchange diffchange-inline">per OD </ins>shown by the reference curve <ins class="diffchange diffchange-inline">indicated </ins>in green.</div></td></tr>
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</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=349279&oldid=prevLnadine at 20:14, 17 October 20142014-10-17T20:14:34Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:<del class="diffchange diffchange-inline">center</del>|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:<ins class="diffchange diffchange-inline">left</ins>|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=348961&oldid=prevLnadine at 20:10, 17 October 20142014-10-17T20:10:42Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:center|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:center|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=348835&oldid=prevLnadine at 20:08, 17 October 20142014-10-17T20:08:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'<ins class="diffchange diffchange-inline">;text-align:center</ins>|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=348751&oldid=prevLnadine at 20:07, 17 October 20142014-10-17T20:07:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=348478&oldid=prevLnadine at 20:04, 17 October 20142014-10-17T20:04:17Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 20:04, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|colspan="4" style='font-size:10pt'|</del>In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=348316&oldid=prevLnadine at 20:02, 17 October 20142014-10-17T20:02:26Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In <del class="diffchange diffchange-inline">all the measurements conducted to create </del>this <del class="diffchange diffchange-inline">matrix </del>the promoter <del class="diffchange diffchange-inline">pLux </del>was the <del class="diffchange diffchange-inline">basis and </del>was <del class="diffchange diffchange-inline">induced in six different variations shown</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In this <ins class="diffchange diffchange-inline">set of experiment </ins>the promoter <ins class="diffchange diffchange-inline">pRhl </ins>was <ins class="diffchange diffchange-inline">tested for possible crosstalk. In </ins>the <ins class="diffchange diffchange-inline">top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour </ins>was <ins class="diffchange diffchange-inline">observed at a concentration of the inducer molecule of 10<sup>3</sup> nM</ins>. <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|<del class="diffchange diffchange-inline">The dark blue points in </del>the <del class="diffchange diffchange-inline">graph top left show </del>the <del class="diffchange diffchange-inline">activation </del>of <del class="diffchange diffchange-inline">gene expression when pLux is induced by </del>3OC6-HSL <del class="diffchange diffchange-inline">(Lux-AHL) </del>binding <del class="diffchange diffchange-inline">to </del>the <del class="diffchange diffchange-inline">corresponding LuxR </del>regulator. The <del class="diffchange diffchange-inline">observed </del>transition <del class="diffchange diffchange-inline">occurs </del>at a concentration of <del class="diffchange diffchange-inline">approximately 1 </del>nM <del class="diffchange diffchange-inline">3OC6-HSL</del>. The <del class="diffchange diffchange-inline">light-blue curve plotted shows modeling data </del>of <del class="diffchange diffchange-inline">pLux induced by 3OC6</del>-HSL <del class="diffchange diffchange-inline">(Lux-AHL) </del>binding to the corresponding <del class="diffchange diffchange-inline">LuxR </del>regulator<del class="diffchange diffchange-inline">. This curve from the model and </del>the <del class="diffchange diffchange-inline">dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux</del>. <del class="diffchange diffchange-inline">Crosstalk can be observed for the cases where </del>the 3OC12-HSL <del class="diffchange diffchange-inline">(Las-AHL) binds </del>the <del class="diffchange diffchange-inline">LuxR regulator</del>. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|<ins class="diffchange diffchange-inline">In </ins>the <ins class="diffchange diffchange-inline">case of 3OC12-HSL binding </ins>the <ins class="diffchange diffchange-inline">RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Severe crosstalk was detected in the case </ins>of 3OC6-HSL binding the <ins class="diffchange diffchange-inline">RhlR </ins>regulator <ins class="diffchange diffchange-inline">followed by induction of pRhl</ins>. The transition <ins class="diffchange diffchange-inline">occurred </ins>at a concentration of <ins class="diffchange diffchange-inline">the inducer molecule of 10<sup>3</sup> </ins>nM <ins class="diffchange diffchange-inline">but compared to the reference curve a lower (1000 AU) value of fluorescence was observed</ins>. The <ins class="diffchange diffchange-inline">second case </ins>of <ins class="diffchange diffchange-inline">crosstalk with the pRhl was detected with 3OC12</ins>-HSL binding to the corresponding <ins class="diffchange diffchange-inline">LasR </ins>regulator <ins class="diffchange diffchange-inline">followed by inducing </ins>the <ins class="diffchange diffchange-inline">promoter pRhl</ins>. <ins class="diffchange diffchange-inline">There </ins>the <ins class="diffchange diffchange-inline">switching occurred at a concentration 1nM of </ins>3OC12-HSL <ins class="diffchange diffchange-inline">reaching a fluorescence of 750 AU. This is approximately 0.5 fold </ins>the <ins class="diffchange diffchange-inline">value of the fluorescence shown by the reference curve in green</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|-</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|The promoter pLux was not tested in combination with the RhlR regulator and the C4-HSL. Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|-</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerRhl.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerRhl.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=346383&oldid=prevLnadine at 19:45, 17 October 20142014-10-17T19:45:11Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:45, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|The promoter pLux was not tested in combination with the RhlR regulator and the C4-HSL. Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerRhl.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerRhl.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_C4-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_C4-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-prhl&diff=337533&oldid=prevEledieu at 17:46, 17 October 20142014-10-17T17:46:43Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 17:46, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-<del class="diffchange diffchange-inline">table_Corner</del>.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-<ins class="diffchange diffchange-inline">table_CornerRhl</ins>.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_C4-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_C4-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]</div></td></tr>
</table>Eledieu