http://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&feed=atom&action=historyTeam:ETH Zurich/expresults/qs/tab-plux - Revision history2024-03-29T04:54:33ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=357341&oldid=prevLnadine at 21:40, 17 October 20142014-10-17T21:40:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter pLux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter pLux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:left|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:left|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In all the measurements conducted to create this matrix the promoter pLux <del class="diffchange diffchange-inline">(ref registry same as above) </del>was the basis and was induced in six different variations shown. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In all the measurements conducted to create this matrix the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>promoter pLux<ins class="diffchange diffchange-inline">] </ins>was the basis and was induced in six different variations shown. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM of 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator <del class="diffchange diffchange-inline">(link to registry)</del>. This curve from the model and the dark blue data points obtained from experiments were plotted as a reference in all the other graphs describing pLux<del class="diffchange diffchange-inline">(ref registry)</del>. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR <del class="diffchange diffchange-inline">(link to registry)</del>regulator. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>pLux<ins class="diffchange diffchange-inline">] </ins>is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0062 </ins>LuxR regulator<ins class="diffchange diffchange-inline">]</ins>. The observed transition occurs at a concentration of approximately 1 nM of 3OC6-HSL. The light-blue curve plotted shows modeling data of <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>pLux<ins class="diffchange diffchange-inline">] </ins>induced by 3OC6-HSL (Lux-AHL) binding to the corresponding <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0062 </ins>LuxR regulator<ins class="diffchange diffchange-inline">]</ins>. This curve from the model and the dark blue data points obtained from experiments were plotted as a reference in all the other graphs describing <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>pLux<ins class="diffchange diffchange-inline">]</ins>. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0062 </ins>LuxR regulator<ins class="diffchange diffchange-inline">]</ins>. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0179 </ins>regulator LasR<ins class="diffchange diffchange-inline">] </ins>and then binding to the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>pLux<ins class="diffchange diffchange-inline">] </ins>as seen in the middle of the top row and center of the matrix. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For the case of Las-AHL binding the LasR <del class="diffchange diffchange-inline">(ref)</del>and subsequently the promoter pLux<del class="diffchange diffchange-inline">(ref)</del>, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux <del class="diffchange diffchange-inline">(ref) </del>induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed, meaning that the ON-OFF-ratio is not significantly different from the reference curve. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For the case of Las-AHL binding the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0179 regulator </ins>LasR<ins class="diffchange diffchange-inline">] </ins>and subsequently the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>promoter pLux<ins class="diffchange diffchange-inline">]</ins>, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>pLux<ins class="diffchange diffchange-inline">] </ins>induced by 3OC6-HSL binding <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0062 </ins>LuxR<ins class="diffchange diffchange-inline">]</ins>. In the case of 3OC12-HSL binding <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0062 </ins>LuxR<ins class="diffchange diffchange-inline">] </ins>and inducing the promoter <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>pLux<ins class="diffchange diffchange-inline">]</ins>, the transition is observed at approximately 100 nM and severe crosstalk is observed, meaning that the ON-OFF-ratio is not significantly different from the reference curve. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR <del class="diffchange diffchange-inline">regulators </del>binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs. However, RhlR induced with its corresponding inducer (C4-HSL) binds to pLux <del class="diffchange diffchange-inline">(ref) </del>and activates expression of GFP at about 100 nM. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Observation of C4-HSL has shown, that there is no significant crosstalk with the <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0062 </ins>LuxR <ins class="diffchange diffchange-inline">regulator] </ins>and <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0179 </ins>LasR <ins class="diffchange diffchange-inline">regulator] </ins>binding C4-HSL and subsequently to <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>pLux<ins class="diffchange diffchange-inline">]</ins>. This is indicated on top right and middle right graphs. However, <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_C0171 </ins>RhlR<ins class="diffchange diffchange-inline">] </ins>induced with its corresponding inducer (C4-HSL) binds to <ins class="diffchange diffchange-inline">[http://parts.igem.org/Part:BBa_R0062 </ins>pLux<ins class="diffchange diffchange-inline">] </ins>and activates expression of GFP at about 100 nM. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=352815&oldid=prevEledieu at 20:51, 17 October 20142014-10-17T20:51:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter pLux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter pLux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
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</table>Eledieuhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=352677&oldid=prevEledieu at 20:49, 17 October 20142014-10-17T20:49:49Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{|class="wikitable <ins class="diffchange diffchange-inline">resized</ins>" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter pLux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter pLux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
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</table>Eledieuhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=351901&oldid=prevLnadine at 20:41, 17 October 20142014-10-17T20:41:44Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter <del class="diffchange diffchange-inline">plux </del>([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter <ins class="diffchange diffchange-inline">pLux </ins>([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:left|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:left|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data <del class="diffchange diffchange-inline">point gained in </del>experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In all the measurements conducted to create this matrix the promoter pLux <ins class="diffchange diffchange-inline">(ref registry same as above) </ins>was the basis and was induced in six different variations shown. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed<del class="diffchange diffchange-inline">.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM <ins class="diffchange diffchange-inline">of </ins>3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator <ins class="diffchange diffchange-inline">(link to registry)</ins>. This curve from the model and the dark blue data <ins class="diffchange diffchange-inline">points obtained from </ins>experiments were plotted as a reference in all the other graphs describing pLux<ins class="diffchange diffchange-inline">(ref registry)</ins>. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR <ins class="diffchange diffchange-inline">(link to registry)</ins>regulator. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The promoter pLux was not tested in combination with </del>the <del class="diffchange diffchange-inline">RhlR regulator and the C4</del>-<del class="diffchange diffchange-inline">HSL</del>. Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For the case of Las-AHL binding the LasR <ins class="diffchange diffchange-inline">(ref)</ins>and subsequently the promoter pLux<ins class="diffchange diffchange-inline">(ref)</ins>, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux <ins class="diffchange diffchange-inline">(ref) </ins>induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed<ins class="diffchange diffchange-inline">, meaning that </ins>the <ins class="diffchange diffchange-inline">ON</ins>-<ins class="diffchange diffchange-inline">OFF-ratio is not significantly different from the reference curve</ins>. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs<ins class="diffchange diffchange-inline">. However, RhlR induced with its corresponding inducer (C4-HSL) binds to pLux (ref) and activates expression of GFP at about 100 nM</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=349344&oldid=prevLnadine at 20:15, 17 October 20142014-10-17T20:15:03Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:<del class="diffchange diffchange-inline">center</del>|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt';text-align:<ins class="diffchange diffchange-inline">left</ins>|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=348894&oldid=prevLnadine at 20:09, 17 October 20142014-10-17T20:09:45Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:09, 17 October 2014</td>
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<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'<ins class="diffchange diffchange-inline">;text-align:center</ins>|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=348556&oldid=prevLnadine at 20:05, 17 October 20142014-10-17T20:05:09Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:05, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|colspan="4" style='font-size:10pt'|</del>The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The promoter pLux was not tested in combination with the RhlR regulator and the C4-HSL. Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|colspan="4" style='font-size:10pt'|</del>Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|colspan="4" style='font-size:10pt'|</del>The promoter pLux was not tested in combination with the RhlR regulator and the C4-HSL. Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=344780&oldid=prevEledieu at 19:24, 17 October 20142014-10-17T19:24:27Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
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<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:24, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|The promoter pLux was not tested in combination with the RhlR regulator and the C4-HSL. Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td></tr>
</table>Eledieuhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=344487&oldid=prevSumana at 19:20, 17 October 20142014-10-17T19:20:14Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:20, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|-</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|-</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|-</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|colspan="4" style='font-size:10pt'|The promoter pLux was not tested in combination with the RhlR regulator and the C4-HSL. Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|-</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]</div></td></tr>
</table>Sumanahttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/expresults/qs/tab-plux&diff=344468&oldid=prevEledieu at 19:19, 17 October 20142014-10-17T19:19:51Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:19, 17 October 2014</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{|class="wikitable <del class="diffchange diffchange-inline">style</del>"="background-color: white; text-align:center; width:auto; margin: auto;"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{|class="wikitable" <ins class="diffchange diffchange-inline">style</ins>="background-color: white; text-align:center; width:auto; margin: auto;"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style='font-size:10pt'|In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. </div></td></tr>
</table>Eledieu