Team:ETH Zurich/achievements

From 2014.igem.org

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=== [https://2014.igem.org/Team:ETH_Zurich/expresults#Quorum Characterizing and reducing leakiness in quorum sensing systems] ===
=== [https://2014.igem.org/Team:ETH_Zurich/expresults#Quorum Characterizing and reducing leakiness in quorum sensing systems] ===
[[Team:ETH_Zurich/expresults#Riboregulators|We quantitatively characterized]] leakiness of the three quorum sensing systems (Las, Lux and Rhl). We have designed a new BioBrick for reducing leakiness of these systems and demonstrated its efficiency for the ''lux'' system, which is the most leaky of the three. We improved the signal over noise ratio for this promoter by a factor of 6 while reducing the basal expression by a factor of 60.
[[Team:ETH_Zurich/expresults#Riboregulators|We quantitatively characterized]] leakiness of the three quorum sensing systems (Las, Lux and Rhl). We have designed a new BioBrick for reducing leakiness of these systems and demonstrated its efficiency for the ''lux'' system, which is the most leaky of the three. We improved the signal over noise ratio for this promoter by a factor of 6 while reducing the basal expression by a factor of 60.
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[[File:ETH_Zurich_2014_PDMS_diffusion_chip_final.jpeg|300px|left|link=https://2014.igem.org/Team:ETH_Zurich/lab/chip]]
=== [https://2014.igem.org/Team:ETH_Zurich/lab/chip Novel millifluidic chip construction method] ===
=== [https://2014.igem.org/Team:ETH_Zurich/lab/chip Novel millifluidic chip construction method] ===
We designed a rapid prototyping approach for creating millifluidic chips: we use a 3D-printing device to create the mold for PDMS.
We designed a rapid prototyping approach for creating millifluidic chips: we use a 3D-printing device to create the mold for PDMS.
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[[File:ETH_Zurich_2014_PDMS_diffusion_chip_final.jpeg|300px||link=https://2014.igem.org/Team:ETH_Zurich/lab/chip]]
 
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=== [https://2014.igem.org/Team:ETH_Zurich/lab/chip Bead to bead communication] ===
=== [https://2014.igem.org/Team:ETH_Zurich/lab/chip Bead to bead communication] ===
We accomplished to produce [[Team:ETH_Zurich/lab/bead|alginate encapsulated ''E. coli'' culture beads]]. The cells within the beads could survive during the time of [[Team:ETH_Zurich/lab/chip#Time-Lapse_Movies|our assays]] and still produce green fluorescent proteins.
We accomplished to produce [[Team:ETH_Zurich/lab/bead|alginate encapsulated ''E. coli'' culture beads]]. The cells within the beads could survive during the time of [[Team:ETH_Zurich/lab/chip#Time-Lapse_Movies|our assays]] and still produce green fluorescent proteins.

Revision as of 01:32, 18 October 2014

iGEM ETH Zurich 2014