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Team:EPF Lausanne/Data
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DATA
Bacterial Biosensors
General Objective: Develop a fast and specific biosensor allowing spatiotemporal analysis of stimuli
To achieve the objective mentioned above, we put forward the following hypothesis:
Hypothesis: Combining protein complementation techniques (split IFP1.4) to proteins going through dimerization (CpxR) upon activation due to a given stimuli (enveloppe stress) allows fast and specific spatiotemporal analysis of a stimuli.
To validate this hypothesis we set ourselves three intermediate objectives:
First intermediate objective: explore if and how CpxR dimerizes in live E.Coli by analyzing the various orientations of CpxR - IFP1.4 fusions.
Second intermediate objective: Analyse the temporal dynamics of the functional CpxR - IFP1.4 fusion protein by change of media.
Third intermediate objective: Analyse the spatial dynamics of the functional CpxR - IFP1.4 fusion protein by microscopy.
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