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Team:EPF Lausanne/Data
From 2014.igem.org
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To validate this hypothesis we set ourselves three intermediate objectives: | To validate this hypothesis we set ourselves three intermediate objectives: | ||
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| - | <u>First intermediate objective: explore if and how CpxR dimerizes in live E.Coli by analyzing the various orientations of CpxR - IFP1.4 fusions </ | + | <u>First intermediate objective:</u> explore if and how CpxR dimerizes in live E.Coli by analyzing the various orientations of CpxR - IFP1.4 fusions. |
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| + | <u>Second intermediate objective:</u> Analyse the temporal dynamics of the functional CpxR - IFP1.4 fusion protein by change of media. | ||
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| + | <u>Third intermediate objective:</u> Analyse the spatial dynamics of the functional CpxR - IFP1.4 fusion protein by microscopy. | ||
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Revision as of 23:14, 11 October 2014
DATA
Bacterial Biosensors
General Objective: Develop a fast and specific biosensor allowing spatiotemporal analysis of stimuli
To achieve the objective mentioned above, we put forward the following hypothesis:
Hypothesis: Combining protein complementation techniques (split IFP1.4) to proteins going through dimerization (CpxR) upon activation due to a given stimuli (enveloppe stress) allows fast and specific spatiotemporal analysis of a stimuli
To validate this hypothesis we set ourselves three intermediate objectives:
First intermediate objective: explore if and how CpxR dimerizes in live E.Coli by analyzing the various orientations of CpxR - IFP1.4 fusions.
Second intermediate objective: Analyse the temporal dynamics of the functional CpxR - IFP1.4 fusion protein by change of media.
Third intermediate objective: Analyse the spatial dynamics of the functional CpxR - IFP1.4 fusion protein by microscopy.
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