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Team:EPF Lausanne/Data
From 2014.igem.org
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To validate this hypothesis we set ourselves three intermediate objectives: | To validate this hypothesis we set ourselves three intermediate objectives: | ||
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<u>First intermediate objective: explore if and how CpxR dimerizes in live E.Coli by analyzing the various orientations of CpxR - IFP1.4 fusions </u> | <u>First intermediate objective: explore if and how CpxR dimerizes in live E.Coli by analyzing the various orientations of CpxR - IFP1.4 fusions </u> | ||
Revision as of 23:11, 11 October 2014
DATA
Bacterial Biosensors
General Objective: Develop a fast and specific biosensor allowing spatiotemporal analysis of stimuli
To achieve the objective mentioned above, we put forward the following hypothesis:
Hypothesis: Combining protein complementation techniques (split IFP1.4) to proteins going through dimerization (CpxR) upon activation due to a given stimuli (enveloppe stress) allows fast and specific spatiotemporal analysis of a stimuli
To validate this hypothesis we set ourselves three intermediate objectives:
First intermediate objective: explore if and how CpxR dimerizes in live E.Coli by analyzing the various orientations of CpxR - IFP1.4 fusions
Second intermediate objective:
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