Team:EPF Lausanne/Attributions

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Acknowledgements

General support

Mrs Marie-France Radigois helped us with the administrative part of the project.

Mr André Pexieder let us use his beloved lab for our project.

Mrs Celia Lutherbacher helped us polish the documentation.

Laboratory techniques

Mr Arne Seitz and Mr José Artacho taught us how to use the microscopes at EPFL's Bioimaging and Optics Facility

Mr Marc Chambon taught us how to use the plate readers at EPFL's Biomolecular Screening Facility.

Mr Julien Dorsaz and Mr Kaspar Suter trained us on the different clean room processes we needed to know to make the masks and wafers for our microfluidic chips. You can find more information on EPFL's center of micronanotechnology here.

Ms Luisa Spisak from EPFL 2013 team helped us starting the project by showing us basic lab techniques.

Yeast handling and transformation techniques

Prof. Simanis and Andrea Krapp helped us a lot to begin the yeast part of the project. They clarified many points regarding yeast transformations and gene regulation in S. Cerevisiae. They confirmed the feasibility of our project and validated the constructs we had designed.

Mr Jean-Daniel, working in our adivsor Bart Deplanck's lab, gave us the ym4271 yeast strain as well as several protocols for yeast selective and unselective medium preparation.

Collaboration

Professor Stephen Michnick and Jacqueline Kowarzyk sent us their split IFP plasmids, which we extensively used to test CpxR dimerization.

Dr Stavroula Hatzios, Postdoc in Professor Waldor's laboratory sent us her renilla luciferase plasmids with CheY/CheZ, which helped us test the luminescence.

Human practices support

Mr Yann Heurtaux, Mr Clément Epié, and Mr Nicolas Loubet, are co-founders of biohackerspaces in France and Switzerland. They sought us out and came to visit, leading to presentations about iGEM and our project at the Hackuarium in Lausanne.

Mr Fabien Goubet is a journalist for "Le Temps" newspaper. He came to visit us at EPFL and wrote an article about our project.

Mr Yves Deluz, general director of the highschools from Canton de Vaud, sent us 4 classes of high school students for a day of discovery of synthetic biology at EPFL.

Mrs Françoise Bezençon, Mr Fabio Moriggia, Mr Jean-Pierre Lardet and Mr Nabil Mastour, highschool biology teachers, collaborated with us and came with their students for the visit at EPFL.

Sponsors