Team:DTU-Denmarkgogo
From 2014.igem.org
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- | <th colspan="2" scope="col"><h1> | + | <th colspan="2" scope="col"><h1>Restriction analysis</h1></th> |
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- | <li> | + | <li>Make a mastermix containing.</li> |
- | <li> | + | <ul> |
- | <li> | + | <li>1X NEBuffer (according to the desired restriction enzyme)</li> |
+ | <li>1X BSA (note if NEBuffer is called X.1 BSA is already added in the buffer)</li> | ||
+ | <li>1 unit/μl restriction enzyme</li> | ||
+ | |||
+ | </ul> | ||
+ | <li>Divide the mastermix into tubes and add DNA to a final concentration of 10 ng/μl</li> | ||
+ | <li>Incubate the reaction mix at the restriction enzymes optimal temperature for 30-60 minutes</li> | ||
+ | <li>Add loading buffer to samples and run on 1% agarose gel with ethidium bromide 0.2 μg/ml</li> | ||
+ | <li>Analyze your gel</li> | ||
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Revision as of 14:25, 1 August 2014
Glycerol stock-preservation |
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