Team:DTU-Denmarkgogo
From 2014.igem.org
(Difference between revisions)
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<table width="100%" border="0" cellspacing="15" cellpadding="0" style="background-color:transparent;"> | <table width="100%" border="0" cellspacing="15" cellpadding="0" style="background-color:transparent;"> | ||
<tr> | <tr> | ||
- | <th colspan="2" scope="col"><h1> | + | <th colspan="2" scope="col"><h1>Recipe on M9 minimal media</h1></th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<ol> | <ol> | ||
- | <li>Add | + | <li>Make M9 salts</li> |
- | <li>Add | + | <li>To make M9 Salts aliquot 800ml H2O and add</li> |
- | <li> | + | <ul> |
+ | <li>64g Na2HPO4-7H2O</li> | ||
+ | <li>15g KH2PO4</li> | ||
+ | <li>2.5g NaCl</li> | ||
+ | <li>5.0g NH4Cl</li> | ||
+ | <li>Stir until dissolved</li> | ||
+ | <li>Adjust to 1000ml with distilled H2O</li> | ||
+ | <li>Sterilize by autoclaving</li> | ||
+ | </ul> | ||
+ | <li>Measure ~700ml of distilled H2O (sterile)</li> | ||
+ | <li>Add 200ml of M9 salts</li> | ||
+ | <li>Add 2ml of 1M MgSO4 (sterile)</li> | ||
+ | <li>Add 20 ml of 20% glucose (or other carbon source)</li> | ||
+ | <li>Add 100ul of 1M CaCl2 (sterile)</li> | ||
+ | <li>Adjust to 1000ml with distilled H2O</li> | ||
+ | |||
</ol> | </ol> | ||
Revision as of 14:18, 1 August 2014
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