Team:Cornell/project/wetlab/futurework

From 2014.igem.org

(Difference between revisions)
Line 10: Line 10:
<div class="row">
<div class="row">
<div class="col-md-12 col-xs-18">
<div class="col-md-12 col-xs-18">
-
<h1 style="margin-top: 0px;">Header 1</h1>
+
<h1 style="margin-top: 0px;">Future Work</h1>
In the future, we hope to continue working with the  <i>merT</i>, <i>merP</i>, <i>CBP4</i>, and <i>nixA</i> heavy metal transport genes by incorporating them upstream of the metallothionein gene <i>GST-YMT</i>. Once each heavy metal transport gene is combined with the metallothionein gene, we can transform the high copy bacterial plasmid into  <i>E. coli</i>. We will then be able to conduct a series of growth assays between our engineered bacteria and <i>E. coli</i> in the presence of heavy metal contaminated water.  
In the future, we hope to continue working with the  <i>merT</i>, <i>merP</i>, <i>CBP4</i>, and <i>nixA</i> heavy metal transport genes by incorporating them upstream of the metallothionein gene <i>GST-YMT</i>. Once each heavy metal transport gene is combined with the metallothionein gene, we can transform the high copy bacterial plasmid into  <i>E. coli</i>. We will then be able to conduct a series of growth assays between our engineered bacteria and <i>E. coli</i> in the presence of heavy metal contaminated water.  
<br><br>
<br><br>
Line 26: Line 26:
</div>
</div>
</div>
</div>
-
<div class="row">
+
-
<div class="col-md-3 col-xs-5">
+
-
<div class="thumbnail">
+
-
<img src="http://igem.org/wiki/images/6/67/IGEMsmall.png" alt="...">
+
-
</div>
+
-
</div>
+
-
<div class="col-md-9 col-xs-15">
+
-
Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts. Separated they live in Bookmarksgrove right at the coast of the Semantics, a large language ocean. A small river named Duden flows by their place and supplies it with the necessary regelialia.
+
-
<br><br>
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="col-md-12 col-xs-18">
+
-
But I must explain to you how all this mistaken idea of denouncing pleasure and praising pain was born and I will give you a complete account of the system, and expound the actual teachings of the great explorer of the truth, the master-builder of human happiness.
+
-
<br><br>
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="col-md-12 col-xs-18">
+
-
<h1>Header 2</h1>
+
-
The quick, brown fox jumps over a lazy dog. DJs flock by when MTV ax quiz prog. Junk MTV quiz graced by fox whelps. Bawds jog, flick quartz, vex nymphs. Waltz, bad nymph, for quick jigs vex! Fox nymphs grab quick-jived waltz. Brick quiz whangs jumpy veldt fox. Bright vixens jump; dozy fowl quack.
+
-
<br><br>
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="col-md-12 col-xs-18">
+
-
<div class="thumbnail">
+
-
<img src="http://igem.org/wiki/images/6/67/IGEMsmall.png" alt="...">
+
-
</div>
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="col-md-12 col-xs-18">
+
-
No one rejects, dislikes, or avoids pleasure itself, because it is pleasure, but because those who do not know how to pursue pleasure rationally encounter consequences that are extremely painful. Nor again is there anyone who loves or pursues or desires to obtain pain of itself, because it is pain, but because occasionally circumstances occur in which toil and pain can procure him some great pleasure. To take a trivial example, which of us ever undertakes laborious physical exercise, except to obtain some advantage from it? But who has any right to find fault with a man who chooses to enjoy a pleasure that has no annoying consequences, or one who avoids a pain that produces no resultant pleasure? On the other hand, we denounce with righteous indignation and dislike men who are so beguiled and demoralized by the charms of pleasure of the moment, so blinded by desire, that they cannot foresee.
+
-
<br><br>
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="col-md-4 col-xs-6">
+
-
<div class="thumbnail">
+
-
<img src="http://igem.org/wiki/images/6/67/IGEMsmall.png" alt="...">
+
-
<div class="caption">
+
-
<h3>Image caption</h3>
+
-
<p>More detail. Stuff stuff stuff stuff.</p>
+
-
<p><a href="#" class="btn btn-primary" role="button">Button</a> <a href="#" class="btn btn-default" role="button">Button</a></p>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
<div class="col-md-4 col-xs-6">
+
-
<div class="thumbnail">
+
-
<img src="http://igem.org/wiki/images/6/67/IGEMsmall.png" alt="...">
+
-
<div class="caption">
+
-
<h3>Image caption</h3>
+
-
<p>More detail. Stuff stuff stuff stuff.</p>
+
-
<p><a href="#" class="btn btn-primary" role="button">Button</a> <a href="#" class="btn btn-default" role="button">Button</a></p>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
<div class="col-md-4 col-xs-6">
+
-
<div class="thumbnail">
+
-
<img src="http://igem.org/wiki/images/6/67/IGEMsmall.png" alt="...">
+
-
<div class="caption">
+
-
<h3>Image caption</h3>
+
-
<p>More detail. Stuff stuff stuff stuff.</p>
+
-
<p><a href="#" class="btn btn-primary" role="button">Button</a> <a href="#" class="btn btn-default" role="button">Button</a></p>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="col-md-12 col-xs-18">
+
-
<h1>Header 3</h1>
+
-
The quick, brown fox jumps over a lazy dog. DJs flock by when MTV ax quiz prog. Junk MTV quiz graced by fox whelps. Bawds jog, flick quartz, ex nymphs. Waltz, bad nymph, for quick jigs vex! Fox nymphs grab quick-jived waltz. Brick quiz whangs jumpy veldt fox. Bright vixens jump; dozy fowl quack.
+
-
<br><br>
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="col-md-12 col-xs-18">
+
-
<h1>References</h1>
+
-
<hr>
+
-
<ol>
+
-
<li>Ref 1</li>
+
-
<li>Ref 2</li>
+
-
<li>Ref 3</li>
+
-
<br><br>
+
-
</div>
+
</div>
</div>
</div>
</div>

Revision as of 01:31, 16 October 2014

Cornell iGEM

web stats

Wet Lab

Future Work

In the future, we hope to continue working with the merT, merP, CBP4, and nixA heavy metal transport genes by incorporating them upstream of the metallothionein gene GST-YMT. Once each heavy metal transport gene is combined with the metallothionein gene, we can transform the high copy bacterial plasmid into E. coli. We will then be able to conduct a series of growth assays between our engineered bacteria and E. coli in the presence of heavy metal contaminated water.

We also hope to continue working on synthesizing a reporter system. In order to detect the saturation of metallothionein sequestering cultures, we plan on using amilCP behind the nickel/cobalt activated promoter Prcn and the mercury activated promoter PmerT. It would be useful to place amilCP behind a lead activated promoter. This system should be incorporated into the BioBrick backbone and transformed into E. coli reporter cultures. These would theoretically be placed into a second hollow fiber reactor that would be connected downstream to the transporter-metallothionein hollow fiber reactor. Effluent water carrying unsequestered metal ions would induce the reporter culture to express amilCP, producing a gradient of blue. We can then test water samples with different heavy metal concentrations to correlate effluent levels against the cultures’ color gradient.

...