Team:Cornell/notebook/plasmids

From 2014.igem.org

(Difference between revisions)
 
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  <tr><td>pA14G</td><td>invalid</td><td>A-Promoter+RBS+merT+merP</td><td>A+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
  <tr><td>pA14G</td><td>invalid</td><td>A-Promoter+RBS+merT+merP</td><td>A+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
  <tr><td>pC14H</td><td>BBa_J04450</td><td>mRFP in pSB1C3</td><td>pC13F</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14H</td><td>BBa_J04450</td><td>mRFP in pSB1C3</td><td>pC13F</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14I</td><td>unsubmitted</td><td>A-Promoter+RBS+merT+merP+ter BioBrick</td><td>AG+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14I</td><td>not submitted</td><td>A-Promoter+RBS+merT+merP+ter BioBrick</td><td>AG+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pA14J</td><td>invalid</td><td>A-Promoter+RBS+nixA</td><td>C+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
  <tr><td>pA14J</td><td>invalid</td><td>A-Promoter+RBS+nixA</td><td>C+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
-
  <tr><td>pC14K</td><td>unsubmitted</td><td>A-Promoter+RBS+nixA+ter BioBrick</td><td>AF+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14K</td><td>not submitted</td><td>A-Promoter+RBS+nixA+ter BioBrick</td><td>AF+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pAK14L</td><td>invalid</td><td>T7+GST-YMT</td><td>B+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
  <tr><td>pAK14L</td><td>invalid</td><td>T7+GST-YMT</td><td>B+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
-
  <tr><td>pC14M</td><td>unsubmitted</td><td>T7+GST-YMT+ter in pSB1C3</td><td>L+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14M</td><td>not submitted</td><td>T7+GST-YMT+ter in pSB1C3</td><td>L+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pAK14N</td><td>invalid</td><td>T7+GST-PMT</td><td>D+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
  <tr><td>pAK14N</td><td>invalid</td><td>T7+GST-PMT</td><td>D+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
-
  <tr><td>pC14O</td><td>unsubmitted</td><td>T7+GST-PMT+ter</td><td>N+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14O</td><td>not submitted</td><td>T7+GST-PMT+ter</td><td>N+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14P</td><td>unsubmitted</td><td>T7+GST-YMT BioBrick</td><td>M</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14P</td><td>not submitted</td><td>T7+GST-YMT BioBrick</td><td>M</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14Q</td><td>unsubmitted</td><td>T7+GST-PMT BioBrick</td><td>O</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14Q</td><td>not submitted</td><td>T7+GST-PMT BioBrick</td><td>O</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pA14R</td><td>invalid</td><td>A-Promoter+RBS+ CBP4 + Biobrick prefix and suffix</td><td>BioBasic</td><td>pUC57</td><td>Ampicillin</td></tr>
  <tr><td>pA14R</td><td>invalid</td><td>A-Promoter+RBS+ CBP4 + Biobrick prefix and suffix</td><td>BioBasic</td><td>pUC57</td><td>Ampicillin</td></tr>
-
  <tr><td>pC14S</td><td>unsubmitted</td><td>A-promoter+RBS+CBP4 BioBrick</td><td>R+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14S</td><td>not submitted</td><td>A-promoter+RBS+CBP4 BioBrick</td><td>R+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14T</td><td>BBa_B1006</td><td>Terminator</td><td>2013 KP: 6D</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14T</td><td>BBa_B1006</td><td>Terminator</td><td>2013 KP: 6D</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14U</td><td>unsubmitted</td><td>Final mercury + GST-YMT construct</td><td>I+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14U</td><td>not submitted</td><td>Final mercury + GST-YMT construct</td><td>I+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14V</td><td>unsubmitted</td><td>Final nickel + GST-YMT construct</td><td>K+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14V</td><td>not submitted</td><td>Final nickel + GST-YMT construct</td><td>K+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14W</td><td>unsubmitted</td><td>Final lead + GST-YMT construct</td><td>S+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14W</td><td>not submitted</td><td>Final lead + GST-YMT construct</td><td>S+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14X</td><td>unsubmitted</td><td>Final mercury + GST-PMT construct</td><td>I+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14X</td><td>not submitted</td><td>Final mercury + GST-PMT construct</td><td>I+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14Y</td><td>unsubmitted</td><td>Final nickel + GST-PMT construct</td><td>K+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14Y</td><td>not submitted</td><td>Final nickel + GST-PMT construct</td><td>K+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14Z</td><td>unsubmitted</td><td>Final lead + GST-PMT construct</td><td>S+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14Z</td><td>not submitted</td><td>Final lead + GST-PMT construct</td><td>S+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14AA</td><td>BBa_K540001</td><td>Nickel inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14AA</td><td>BBa_K540001</td><td>Nickel inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14AB</td><td>BBa_K346002</td><td>mercury inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14AB</td><td>BBa_K346002</td><td>mercury inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14AC</td><td>BBa_K206000</td><td>pBAD</td><td>2014 KP3: 14A</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14AC</td><td>BBa_K206000</td><td>pBAD</td><td>2014 KP3: 14A</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14AD</td><td>unsubmitted</td><td>Nickel inducible + mRFP</td><td>AA+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14AD</td><td>not submitted</td><td>Nickel inducible + mRFP</td><td>AA+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14AE</td><td>unsubmitted</td><td>Mercury inducible + mRFP</td><td>AB+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14AE</td><td>not submitted</td><td>Mercury inducible + mRFP</td><td>AB+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pA14AF</td><td>invalid</td><td>NixA Synthesized (A-promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
  <tr><td>pA14AF</td><td>invalid</td><td>NixA Synthesized (A-promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
  <tr><td>pA14AG</td><td>invalid</td><td>MerT/MerP Synthesized (A-Promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
  <tr><td>pA14AG</td><td>invalid</td><td>MerT/MerP Synthesized (A-Promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
  <tr><td>pA14AH</td><td>invalid</td><td>GST-YMT Synthesized (pT7, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
  <tr><td>pA14AH</td><td>invalid</td><td>GST-YMT Synthesized (pT7, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
-
  <tr><td>pC14AI</td><td>unsubmitted</td><td>GST-YMT Synthesized (pT7, RBS, ter) in pSB1C3</td><td>AH+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14AI</td><td>not submitted</td><td>GST-YMT Synthesized (pT7, RBS, ter) in pSB1C3</td><td>AH+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14AJ</td><td>BBa_K592009</td><td>amilCP in pSB1C3</td><td>2014 KP1: 19E</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14AJ</td><td>BBa_K592009</td><td>amilCP in pSB1C3</td><td>2014 KP1: 19E</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><td>pC14AK</td><td>unsubmitted</td><td>GST-YMT Synthesized without T7 (RBS,ter) in pSB1C3</td><td>AI + T7</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14AK</td><td>not submitted</td><td>GST-YMT Synthesized without T7 (RBS,ter) in pSB1C3</td><td>AI + T7</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
</table>
</table>
</div>
</div>

Latest revision as of 01:41, 18 October 2014

Cornell iGEM

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Plasmids

Plasmid NamePartContents/DescriptionOriginBackboneAntibiotic Resistance
pA14ApDH1mer operonWilsonpCL1921Ampicillin
pA14BpGYMTGST-YMTWilsonpGEX-2TAmpicillin
pA14CpUEF202nixAWilsonpBluescriptAmpicillin
pA14DpGPMTGST-PMTWilsonpGPMTAmpicillin
pAK14EBBa_I712074T7 promoterpAK13DpSB1AK8Ampicillin
pA14FBBa_J23102Anderson Promoter+mRFP2013 KP5: 18GpJ61002Ampicillin
pA14GinvalidA-Promoter+RBS+merT+merPA+FpJ61002Ampicillin
pC14HBBa_J04450mRFP in pSB1C3pC13FpSB1C3Chloramphenicol
pC14Inot submittedA-Promoter+RBS+merT+merP+ter BioBrickAG+HpSB1C3Chloramphenicol
pA14JinvalidA-Promoter+RBS+nixAC+FpJ61002Ampicillin
pC14Knot submittedA-Promoter+RBS+nixA+ter BioBrickAF+TpSB1C3Chloramphenicol
pAK14LinvalidT7+GST-YMTB+EpSB1AK8Ampicillin
pC14Mnot submittedT7+GST-YMT+ter in pSB1C3L+TpSB1C3Chloramphenicol
pAK14NinvalidT7+GST-PMTD+EpSB1AK8Ampicillin
pC14Onot submittedT7+GST-PMT+terN+TpSB1C3Chloramphenicol
pC14Pnot submittedT7+GST-YMT BioBrickMpSB1C3Chloramphenicol
pC14Qnot submittedT7+GST-PMT BioBrickOpSB1C3Chloramphenicol
pA14RinvalidA-Promoter+RBS+ CBP4 + Biobrick prefix and suffixBioBasicpUC57Ampicillin
pC14Snot submittedA-promoter+RBS+CBP4 BioBrickR+HpSB1C3Chloramphenicol
pC14TBBa_B1006Terminator2013 KP: 6DpSB1C3Chloramphenicol
pC14Unot submittedFinal mercury + GST-YMT constructI+PpSB1C3Chloramphenicol
pC14Vnot submittedFinal nickel + GST-YMT constructK+PpSB1C3Chloramphenicol
pC14Wnot submittedFinal lead + GST-YMT constructS+PpSB1C3Chloramphenicol
pC14Xnot submittedFinal mercury + GST-PMT constructI+QpSB1C3Chloramphenicol
pC14Ynot submittedFinal nickel + GST-PMT constructK+QpSB1C3Chloramphenicol
pC14Znot submittedFinal lead + GST-PMT constructS+QpSB1C3Chloramphenicol
pC14AABBa_K540001Nickel inducibleKit PlatepSB1C3Chloramphenicol
pC14ABBBa_K346002mercury inducibleKit PlatepSB1C3Chloramphenicol
pC14ACBBa_K206000pBAD2014 KP3: 14ApSB1C3Chloramphenicol
pC14ADnot submittedNickel inducible + mRFPAA+HpSB1C3Chloramphenicol
pC14AEnot submittedMercury inducible + mRFPAB+HpSB1C3Chloramphenicol
pA14AFinvalidNixA Synthesized (A-promoter, RBS, ter)GenScriptpUC57Ampicillin
pA14AGinvalidMerT/MerP Synthesized (A-Promoter, RBS, ter)GenScriptpUC57Ampicillin
pA14AHinvalidGST-YMT Synthesized (pT7, RBS, ter)GenScriptpUC57Ampicillin
pC14AInot submittedGST-YMT Synthesized (pT7, RBS, ter) in pSB1C3AH+HpSB1C3Chloramphenicol
pC14AJBBa_K592009amilCP in pSB1C32014 KP1: 19EpSB1C3Chloramphenicol
pC14AKnot submittedGST-YMT Synthesized without T7 (RBS,ter) in pSB1C3AI + T7pSB1C3Chloramphenicol