Team:Cornell/notebook/plasmids

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<tr class="tableizer-firstrow"><th>Plasmid Name</th><th>Part</th><th>Contents/Description</th><th>Origin</th><th>Backbone</th><th>Antibiotic Resistance</th></tr>
<tr class="tableizer-firstrow"><th>Plasmid Name</th><th>Part</th><th>Contents/Description</th><th>Origin</th><th>Backbone</th><th>Antibiotic Resistance</th></tr>
  <tr><td>pA14A</td><td>pDH1</td><td>mer operon</td><td>Wilson</td><td>pCL1921</td><td>Ampicillin</td></tr>
  <tr><td>pA14A</td><td>pDH1</td><td>mer operon</td><td>Wilson</td><td>pCL1921</td><td>Ampicillin</td></tr>
-
  <tr><tr class="alt"><td>pA14B</td><td>pGYMT</td><td>GST-YMT</td><td>Wilson</td><td>pGEX-2T</td><td>Ampicillin</td></tr>
+
  <tr><td>pA14B</td><td>pGYMT</td><td>GST-YMT</td><td>Wilson</td><td>pGEX-2T</td><td>Ampicillin</td></tr>
  <tr><td>pA14C</td><td>pUEF202</td><td>nixA</td><td>Wilson</td><td>pBluescript</td><td>Ampicillin</td></tr>
  <tr><td>pA14C</td><td>pUEF202</td><td>nixA</td><td>Wilson</td><td>pBluescript</td><td>Ampicillin</td></tr>
-
  <tr><tr class="alt"><td>pA14D</td><td>pGPMT</td><td>GST-PMT</td><td>Wilson</td><td>pGPMT</td><td>Ampicillin</td></tr>
+
  <tr><td>pA14D</td><td>pGPMT</td><td>GST-PMT</td><td>Wilson</td><td>pGPMT</td><td>Ampicillin</td></tr>
  <tr><td>pAK14E</td><td>BBa_I712074</td><td>T7 promoter</td><td>pAK13D</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
  <tr><td>pAK14E</td><td>BBa_I712074</td><td>T7 promoter</td><td>pAK13D</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
-
  <tr><tr class="alt"><td>pA14F</td><td>BBa_J23102</td><td>Anderson Promoter+mRFP</td><td>2013 KP5: 18G</td><td>pJ61002</td><td>Ampicillin</td></tr>
+
  <tr><td>pA14F</td><td>BBa_J23102</td><td>Anderson Promoter+mRFP</td><td>2013 KP5: 18G</td><td>pJ61002</td><td>Ampicillin</td></tr>
  <tr><td>pA14G</td><td>invalid</td><td>A-Promoter+RBS+merT+merP</td><td>A+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
  <tr><td>pA14G</td><td>invalid</td><td>A-Promoter+RBS+merT+merP</td><td>A+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
-
  <tr><tr class="alt"><td>pC14H</td><td>BBa_J04450</td><td>mRFP in pSB1C3</td><td>pC13F</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
+
  <tr><td>pC14H</td><td>BBa_J04450</td><td>mRFP in pSB1C3</td><td>pC13F</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14I</td><td>unsubmitted</td><td>A-Promoter+RBS+merT+merP+ter BioBrick</td><td>AG+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14I</td><td>unsubmitted</td><td>A-Promoter+RBS+merT+merP+ter BioBrick</td><td>AG+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><tr class="alt"><td>pA14J</td><td>invalid</td><td>A-Promoter+RBS+nixA</td><td>C+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
+
  <tr><td>pA14J</td><td>invalid</td><td>A-Promoter+RBS+nixA</td><td>C+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
  <tr><td>pC14K</td><td>unsubmitted</td><td>A-Promoter+RBS+nixA+ter BioBrick</td><td>AF+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14K</td><td>unsubmitted</td><td>A-Promoter+RBS+nixA+ter BioBrick</td><td>AF+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
-
  <tr><tr class="alt"><td>pAK14L</td><td>invalid</td><td>T7+GST-YMT</td><td>B+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
+
  <tr><td>pAK14L</td><td>invalid</td><td>T7+GST-YMT</td><td>B+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
  <tr><td>pC14M</td><td>unsubmitted</td><td>T7+GST-YMT+ter in pSB1C3</td><td>L+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pC14M</td><td>unsubmitted</td><td>T7+GST-YMT+ter in pSB1C3</td><td>L+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
  <tr><td>pAK14N</td><td>invalid</td><td>T7+GST-PMT</td><td>D+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
  <tr><td>pAK14N</td><td>invalid</td><td>T7+GST-PMT</td><td>D+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>

Revision as of 23:50, 15 October 2014

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Plasmids


Plasmid NamePartContents/DescriptionOriginBackboneAntibiotic Resistance
pA14ApDH1mer operonWilsonpCL1921Ampicillin
pA14BpGYMTGST-YMTWilsonpGEX-2TAmpicillin
pA14CpUEF202nixAWilsonpBluescriptAmpicillin
pA14DpGPMTGST-PMTWilsonpGPMTAmpicillin
pAK14EBBa_I712074T7 promoterpAK13DpSB1AK8Ampicillin
pA14FBBa_J23102Anderson Promoter+mRFP2013 KP5: 18GpJ61002Ampicillin
pA14GinvalidA-Promoter+RBS+merT+merPA+FpJ61002Ampicillin
pC14HBBa_J04450mRFP in pSB1C3pC13FpSB1C3Chloramphenicol
pC14IunsubmittedA-Promoter+RBS+merT+merP+ter BioBrickAG+HpSB1C3Chloramphenicol
pA14JinvalidA-Promoter+RBS+nixAC+FpJ61002Ampicillin
pC14KunsubmittedA-Promoter+RBS+nixA+ter BioBrickAF+TpSB1C3Chloramphenicol
pAK14LinvalidT7+GST-YMTB+EpSB1AK8Ampicillin
pC14MunsubmittedT7+GST-YMT+ter in pSB1C3L+TpSB1C3Chloramphenicol
pAK14NinvalidT7+GST-PMTD+EpSB1AK8Ampicillin
pC14OunsubmittedT7+GST-PMT+terN+TpSB1C3Chloramphenicol
pC14PunsubmittedT7+GST-YMT BioBrickMpSB1C3Chloramphenicol
pC14QunsubmittedT7+GST-PMT BioBrickOpSB1C3Chloramphenicol
pA14RinvalidA-Promoter+RBS+ CBP4 + Biobrick prefix and suffixBioBasicpUC57Ampicillin
pC14SunsubmittedA-promoter+RBS+CBP4 BioBrickR+HpSB1C3Chloramphenicol
pC14TBBa_B1006Terminator2013 KP: 6DpSB1C3Chloramphenicol
pC14UunsubmittedFinal mercury + GST-YMT constructI+PpSB1C3Chloramphenicol
pC14VunsubmittedFinal nickel + GST-YMT constructK+PpSB1C3Chloramphenicol
pC14WunsubmittedFinal lead + GST-YMT constructS+PpSB1C3Chloramphenicol
pC14XunsubmittedFinal mercury + GST-PMT constructI+QpSB1C3Chloramphenicol
pC14YunsubmittedFinal nickel + GST-PMT constructK+QpSB1C3Chloramphenicol
pC14ZunsubmittedFinal lead + GST-PMT constructS+QpSB1C3Chloramphenicol
pC14AABBa_K540001Nickel inducibleKit PlatepSB1C3Chloramphenicol
pC14ABBBa_K346002mercury inducibleKit PlatepSB1C3Chloramphenicol
pC14ACBBa_K206000pBAD2014 KP3: 14ApSB1C3Chloramphenicol
pC14ADunsubmittedNickel inducible + mRFPAA+HpSB1C3Chloramphenicol
pC14AEunsubmittedMercury inducible + mRFPAB+HpSB1C3Chloramphenicol
pA14AFinvalidNixA Synthesized (A-promoter, RBS, ter)GenScriptpUC57Ampicillin
pA14AGinvalidMerT/MerP Synthesized (A-Promoter, RBS, ter)GenScriptpUC57Ampicillin
pA14AHinvalidGST-YMT Synthesized (pT7, RBS, ter)GenScriptpUC57Ampicillin
pC14AIunsubmittedGST-YMT Synthesized (pT7, RBS, ter) in pSB1C3AH+HpSB1C3Chloramphenicol
pC14AJBBa_K592009amilCP in pSB1C32014 KP1: 19EpSB1C3Chloramphenicol
pC14AKunsubmittedGST-YMT Synthesized without T7 (RBS,ter) in pSB1C3AI + T7pSB1C3Chloramphenicol