Team:Cornell/notebook/plasmids

From 2014.igem.org

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<h1 style="margin-top: 0px;">Header 1</h1>
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Text text text Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu. In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt.
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<tr class="tableizer-firstrow"><th>Plasmid Name</th><th>Part</th><th>Contents/Description</th><th>Origin</th><th>Backbone</th><th>Antibiotic Resistance</th></tr>
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<tr><td>pA14A</td><td>pDH1</td><td>mer operon</td><td>Wilson</td><td>pCL1921</td><td>Ampicillin</td></tr>
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<tr><td>pA14B</td><td>pGYMT</td><td>GST-YMT</td><td>Wilson</td><td>pGEX-2T</td><td>Ampicillin</td></tr>
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<tr><td>pA14C</td><td>pUEF202</td><td>nixA</td><td>Wilson</td><td>pBluescript</td><td>Ampicillin</td></tr>
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<tr><td>pA14D</td><td>pGPMT</td><td>GST-PMT</td><td>Wilson</td><td>pGPMT</td><td>Ampicillin</td></tr>
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<tr><td>pAK14E</td><td>BBa_I712074</td><td>T7 promoter</td><td>pAK13D</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
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Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts. Separated they live in Bookmarksgrove right at the coast of the Semantics, a large language ocean. A small river named Duden flows by their place and supplies it with the necessary regelialia.
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<tr><td>pA14F</td><td>BBa_J23102</td><td>Anderson Promoter+mRFP</td><td>2013 KP5: 18G</td><td>pJ61002</td><td>Ampicillin</td></tr>
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<tr><td>pA14G</td><td>invalid</td><td>A-Promoter+RBS+merT+merP</td><td>A+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
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<tr><td>pC14H</td><td>BBa_J04450</td><td>mRFP in pSB1C3</td><td>pC13F</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14I</td><td>unsubmitted</td><td>A-Promoter+RBS+merT+merP+ter BioBrick</td><td>AG+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pA14J</td><td>invalid</td><td>A-Promoter+RBS+nixA</td><td>C+F</td><td>pJ61002</td><td>Ampicillin</td></tr>
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<img src="https://static.igem.org/mediawiki/igem.org/6/67/IGEMsmall.png" alt="...">
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<tr><td>pC14K</td><td>unsubmitted</td><td>A-Promoter+RBS+nixA+ter BioBrick</td><td>AF+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pAK14L</td><td>invalid</td><td>T7+GST-YMT</td><td>B+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
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<tr><td>pC14M</td><td>unsubmitted</td><td>T7+GST-YMT+ter in pSB1C3</td><td>L+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pAK14N</td><td>invalid</td><td>T7+GST-PMT</td><td>D+E</td><td>pSB1AK8</td><td>Ampicillin</td></tr>
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<tr><td>pC14O</td><td>unsubmitted</td><td>T7+GST-PMT+ter</td><td>N+T</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14P</td><td>unsubmitted</td><td>T7+GST-YMT BioBrick</td><td>M</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14Q</td><td>unsubmitted</td><td>T7+GST-PMT BioBrick</td><td>O</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<img src="https://static.igem.org/mediawiki/igem.org/6/67/IGEMsmall.png" alt="...">
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<tr><td>pA14R</td><td>invalid</td><td>A-Promoter+RBS+ CBP4 + Biobrick prefix and suffix</td><td>BioBasic</td><td>pUC57</td><td>Ampicillin</td></tr>
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<tr><td>pC14S</td><td>unsubmitted</td><td>A-promoter+RBS+CBP4 BioBrick</td><td>R+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14T</td><td>BBa_B1006</td><td>Terminator</td><td>2013 KP: 6D</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14U</td><td>unsubmitted</td><td>Final mercury + GST-YMT construct</td><td>I+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts. Separated they live in Bookmarksgrove right at the coast of the Semantics, a large language ocean. A small river named Duden flows by their place and supplies it with the necessary regelialia.
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<tr><td>pC14V</td><td>unsubmitted</td><td>Final nickel + GST-YMT construct</td><td>K+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14W</td><td>unsubmitted</td><td>Final lead + GST-YMT construct</td><td>S+P</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14X</td><td>unsubmitted</td><td>Final mercury + GST-PMT construct</td><td>I+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14Y</td><td>unsubmitted</td><td>Final nickel + GST-PMT construct</td><td>K+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14Z</td><td>unsubmitted</td><td>Final lead + GST-PMT construct</td><td>S+Q</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14AA</td><td>BBa_K540001</td><td>Nickel inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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But I must explain to you how all this mistaken idea of denouncing pleasure and praising pain was born and I will give you a complete account of the system, and expound the actual teachings of the great explorer of the truth, the master-builder of human happiness.
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<tr><td>pC14AB</td><td>BBa_K346002</td><td>mercury inducible</td><td>Kit Plate</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14AC</td><td>BBa_K206000</td><td>pBAD</td><td>2014 KP3: 14A</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14AD</td><td>unsubmitted</td><td>Nickel inducible + mRFP</td><td>AA+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14AE</td><td>unsubmitted</td><td>Mercury inducible + mRFP</td><td>AB+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pA14AF</td><td>invalid</td><td>NixA Synthesized (A-promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
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<tr><td>pA14AG</td><td>invalid</td><td>MerT/MerP Synthesized (A-Promoter, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
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<h1>Header 2</h1>
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<tr><td>pA14AH</td><td>invalid</td><td>GST-YMT Synthesized (pT7, RBS, ter)</td><td>GenScript</td><td>pUC57</td><td>Ampicillin</td></tr>
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The quick, brown fox jumps over a lazy dog. DJs flock by when MTV ax quiz prog. Junk MTV quiz graced by fox whelps. Bawds jog, flick quartz, vex nymphs. Waltz, bad nymph, for quick jigs vex! Fox nymphs grab quick-jived waltz. Brick quiz whangs jumpy veldt fox. Bright vixens jump; dozy fowl quack.
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<tr><td>pC14AI</td><td>unsubmitted</td><td>GST-YMT Synthesized (pT7, RBS, ter) in pSB1C3</td><td>AH+H</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14AJ</td><td>BBa_K592009</td><td>amilCP in pSB1C3</td><td>2014 KP1: 19E</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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<tr><td>pC14AK</td><td>unsubmitted</td><td>GST-YMT Synthesized without T7 (RBS,ter) in pSB1C3</td><td>AI - T7</td><td>pSB1C3</td><td>Chloramphenicol</td></tr>
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No one rejects, dislikes, or avoids pleasure itself, because it is pleasure, but because those who do not know how to pursue pleasure rationally encounter consequences that are extremely painful. Nor again is there anyone who loves or pursues or desires to obtain pain of itself, because it is pain, but because occasionally circumstances occur in which toil and pain can procure him some great pleasure. To take a trivial example, which of us ever undertakes laborious physical exercise, except to obtain some advantage from it? But who has any right to find fault with a man who chooses to enjoy a pleasure that has no annoying consequences, or one who avoids a pain that produces no resultant pleasure? On the other hand, we denounce with righteous indignation and dislike men who are so beguiled and demoralized by the charms of pleasure of the moment, so blinded by desire, that they cannot foresee.
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The quick, brown fox jumps over a lazy dog. DJs flock by when MTV ax quiz prog. Junk MTV quiz graced by fox whelps. Bawds jog, flick quartz, ex nymphs. Waltz, bad nymph, for quick jigs vex! Fox nymphs grab quick-jived waltz. Brick quiz whangs jumpy veldt fox. Bright vixens jump; dozy fowl quack.
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<h1 style="margin-bottom: 0px">References</h1>
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Revision as of 23:15, 15 October 2014

Cornell iGEM

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Plasmids

<
Plasmid NamePartContents/DescriptionOriginBackboneAntibiotic Resistance
pA14ApDH1mer operonWilsonpCL1921Ampicillin
pA14BpGYMTGST-YMTWilsonpGEX-2TAmpicillin
pA14CpUEF202nixAWilsonpBluescriptAmpicillin
pA14DpGPMTGST-PMTWilsonpGPMTAmpicillin
pAK14EBBa_I712074T7 promoterpAK13DpSB1AK8Ampicillin
pA14FBBa_J23102Anderson Promoter+mRFP2013 KP5: 18GpJ61002Ampicillin
pA14GinvalidA-Promoter+RBS+merT+merPA+FpJ61002Ampicillin
pC14HBBa_J04450mRFP in pSB1C3pC13FpSB1C3Chloramphenicol
pC14IunsubmittedA-Promoter+RBS+merT+merP+ter BioBrickAG+HpSB1C3Chloramphenicol
pA14JinvalidA-Promoter+RBS+nixAC+FpJ61002Ampicillin
pC14KunsubmittedA-Promoter+RBS+nixA+ter BioBrickAF+TpSB1C3Chloramphenicol
pAK14LinvalidT7+GST-YMTB+EpSB1AK8Ampicillin
pC14MunsubmittedT7+GST-YMT+ter in pSB1C3L+TpSB1C3Chloramphenicol
pAK14NinvalidT7+GST-PMTD+EpSB1AK8Ampicillin
pC14OunsubmittedT7+GST-PMT+terN+TpSB1C3Chloramphenicol
pC14PunsubmittedT7+GST-YMT BioBrickMpSB1C3Chloramphenicol
pC14QunsubmittedT7+GST-PMT BioBrickOpSB1C3Chloramphenicol
pA14RinvalidA-Promoter+RBS+ CBP4 + Biobrick prefix and suffixBioBasicpUC57Ampicillin
pC14SunsubmittedA-promoter+RBS+CBP4 BioBrickR+HpSB1C3Chloramphenicol
pC14TBBa_B1006Terminator2013 KP: 6DpSB1C3Chloramphenicol
pC14UunsubmittedFinal mercury + GST-YMT constructI+PpSB1C3Chloramphenicol
pC14VunsubmittedFinal nickel + GST-YMT constructK+PpSB1C3Chloramphenicol
pC14WunsubmittedFinal lead + GST-YMT constructS+PpSB1C3Chloramphenicol
pC14XunsubmittedFinal mercury + GST-PMT constructI+QpSB1C3Chloramphenicol
pC14YunsubmittedFinal nickel + GST-PMT constructK+QpSB1C3Chloramphenicol
pC14ZunsubmittedFinal lead + GST-PMT constructS+QpSB1C3Chloramphenicol
pC14AABBa_K540001Nickel inducibleKit PlatepSB1C3Chloramphenicol
pC14ABBBa_K346002mercury inducibleKit PlatepSB1C3Chloramphenicol
pC14ACBBa_K206000pBAD2014 KP3: 14ApSB1C3Chloramphenicol
pC14ADunsubmittedNickel inducible + mRFPAA+HpSB1C3Chloramphenicol
pC14AEunsubmittedMercury inducible + mRFPAB+HpSB1C3Chloramphenicol
pA14AFinvalidNixA Synthesized (A-promoter, RBS, ter)GenScriptpUC57Ampicillin
pA14AGinvalidMerT/MerP Synthesized (A-Promoter, RBS, ter)GenScriptpUC57Ampicillin
pA14AHinvalidGST-YMT Synthesized (pT7, RBS, ter)GenScriptpUC57Ampicillin
pC14AIunsubmittedGST-YMT Synthesized (pT7, RBS, ter) in pSB1C3AH+HpSB1C3Chloramphenicol
pC14AJBBa_K592009amilCP in pSB1C32014 KP1: 19EpSB1C3Chloramphenicol
pC14AKunsubmittedGST-YMT Synthesized without T7 (RBS,ter) in pSB1C3AI - T7pSB1C3Chloramphenicol

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