Team:Clemson

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<center><a href="https://2013.igem.org/Team:Lethbridge/project"><image src="https://static.igem.org/mediawiki/2013/0/05/ULeth2013iGEM_Mainpage_PKFig.png" width="600px" height="400px"/></a></center>
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<h2>Project Overview</h2>
 
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<p style="color:black">This year, the Lethbridge iGEM team is working to bring a whole new class of parts to the iGEM community: programmed ribosomal frameshifting elements. To do this, we have been working towards standardizing the PK401 pseudoknot for use within the BioBrick system. These RNA secondary structural elements cause the ribosome to switch between translational frames and give another degree of freedom when engineering genetic circuits.</p><br>
 
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<b style="color:black"><u>WHAT?</u></b>
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<ul style="color:black"><li>Our project is directed towards standardizing pseudoknots to make a new class of parts available to the synthetic biology community</li></ul>  
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<b style="color:black"><u>WHY?</u></b>
 
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<ul style="color:black"><li>As the field of synthetic biology grows, so should its toolset. By introducing a standardized method of implementing programmed ribosomal frameshifts in synthetic gene networks, we could not only enable others to reduce plasmid size and regulate operon expression, but also enable them to come up with new, exciting applications</li></ul>
 
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<b style="color:black"><u>HOW?</b></u>
 
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<ul style="color:black"><li>We have brought pseudoknots to the iGEM community by:
 
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<ul><li>Characterizing their function in a biobrick system</li>
 
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<li>Designing software that enables others to dual code proteins</li>
 
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<li>Ensuring that the release of these tools to the wider public does not pose a significant risk to the rest of the world</li></li></ul></ul><br>
 
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<h2>Sponsors</h2>
 
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<p><center><image src="https://static.igem.org/mediawiki/2013/0/00/ULeth2013_Sponsors_-_Platinum.png"; width="200px"; height="100px" />&nbsp;&nbsp;&nbsp;&nbsp;
 
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<image src="https://static.igem.org/mediawiki/2013/1/1e/ULeth2013_Sponsors_-_Silver.png"; width="200px"; height="100px" />&nbsp;&nbsp;&nbsp;&nbsp;
 
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<image src="https://static.igem.org/mediawiki/2013/4/4c/ULeth2013_Sponsors_-_Bronze.png"; width="300px"; height="100px" /></center></p>&nbsp;&nbsp;&nbsp;&nbsp;
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<tr><td colspan="3"> <h3> Quorum Sensing</h3></td></tr>
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<p>Quorum sensing is a means for bacteria to communicate with one another. This signaling is density-dependent, and when a large enough number of organisms are present, they even coordinate gene expression. A famous example is Vibrio fischeri, which gives the Hawaiian bobtail squid its eerie glow. When a large enough concentration of Vibrio fischeri come together in the squid's light-producing organ, or photophore, they communicate by quorum sensing and all express the luciferase gene, resulting in beautiful bioluminescence.
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Quorum sensing is largely dependent upon the production of N-acyl Homoserine Lactones or AHLs. The 2014 Clemson iGEM Team worked to develop a system to detect the presence of AHLs for better study of quorum sensing and biofilm interactions. This system can be readily adapted to detect any number molecules and thus provide a flexible tool for microbiologists.  </p>
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Latest revision as of 03:08, 18 October 2014

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Retrieved from "http://2014.igem.org/Team:Clemson"

Quorum Sensing

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Quorum sensing is a means for bacteria to communicate with one another. This signaling is density-dependent, and when a large enough number of organisms are present, they even coordinate gene expression. A famous example is Vibrio fischeri, which gives the Hawaiian bobtail squid its eerie glow. When a large enough concentration of Vibrio fischeri come together in the squid's light-producing organ, or photophore, they communicate by quorum sensing and all express the luciferase gene, resulting in beautiful bioluminescence. Quorum sensing is largely dependent upon the production of N-acyl Homoserine Lactones or AHLs. The 2014 Clemson iGEM Team worked to develop a system to detect the presence of AHLs for better study of quorum sensing and biofilm interactions. This system can be readily adapted to detect any number molecules and thus provide a flexible tool for microbiologists.