Team:CityU HK/project/result

From 2014.igem.org

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<h2 class="sub">Experimental Achievements from 15 June – 3 October 2014 :</h2>
<h2 class="sub">Experimental Achievements from 15 June – 3 October 2014 :</h2>
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<p class="content">Successfully constructed a Biobrick for a tetracycline regulated <i>fadL-fadD</i> expression system (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472606</a>).<br><br>
<p class="content">Successfully constructed a Biobrick for a tetracycline regulated <i>fadL-fadD</i> expression system (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472606</a>).<br><br>
Successfully constructed and characterized a T7-regulated <i>fadL-fadD</i>  system in the pSNAP-tag® (T7)-2 expression vector.<br><br>
Successfully constructed and characterized a T7-regulated <i>fadL-fadD</i>  system in the pSNAP-tag® (T7)-2 expression vector.<br><br>
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Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.
Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.
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Revision as of 15:07, 16 October 2014

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Result Summary

Experimental Achievements from 15 June – 3 October 2014 :

Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (BBa_K1472606).

Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.

Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (BBa_K1472601).

Successfully constructed a translational unit of the ‘tesA gene in pAB1A2.

Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.

Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (BBa_K1472610).

Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ12 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ15 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ9-Δ12 desaturase gene cluster in pSB1A2.

Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.



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