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Team:CityU HK/project/module fad - Revision history
2024-03-28T17:46:37Z
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Marialilokyee at 18:17, 17 October 2014
2014-10-17T18:17:25Z
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Marialilokyee
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Marialilokyee at 17:59, 16 October 2014
2014-10-16T17:59:11Z
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Marialilokyee
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Marialilokyee at 17:58, 16 October 2014
2014-10-16T17:58:47Z
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Marialilokyee
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Marialilokyee at 17:58, 16 October 2014
2014-10-16T17:58:11Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h1 id</del>="<del class="diffchange diffchange-inline">title</del>"><del class="diffchange diffchange-inline">Module description</del></<del class="diffchange diffchange-inline">h1><br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">div><img src</ins>="<ins class="diffchange diffchange-inline">https://static.igem.org/mediawiki/2014/f/fc/CityU_HK_module-banner.png" width="100%</ins>"></<ins class="diffchange diffchange-inline">div</ins>></div></td></tr>
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Marialilokyee
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Marialilokyee at 15:00, 16 October 2014
2014-10-16T15:00:10Z
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Marialilokyee
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Kenlaukit at 12:49, 16 October 2014
2014-10-16T12:49:08Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to achieve this, we have sub-divided our project into three parts:<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to achieve this, we have sub-divided our project into three parts:<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b>a.</b> Enhanced uptake of long chain fatty acids (LCFAs) by <i>E. coli</i> <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b>a.</b> Enhanced uptake of long chain fatty acids (LCFAs) by <i>E. coli</i> <ins class="diffchange diffchange-inline"> </ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>b. </b>Increased conversion of fatty acyl-CoA into free fatty acid <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>b. </b>Increased conversion of fatty acyl-CoA into free fatty acid <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>c. </b>Enhanced conversion of free fatty acid into ALA<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>c. </b>Enhanced conversion of free fatty acid into ALA<br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The uptake of exogenous long chain fatty acids is controlled by the fadL and fadD genes.</p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The uptake of exogenous long chain fatty acids is controlled by the <ins class="diffchange diffchange-inline"><i></ins>fadL<ins class="diffchange diffchange-inline"></i> </ins>and <ins class="diffchange diffchange-inline"><i></ins>fadD<ins class="diffchange diffchange-inline"></i> </ins>genes.</p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <th>Figure 1. </th></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <th>Figure 1. </th></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td> Importation of long-chain fatty acids by FadL and FadD. Long chain fatty acids (LCFAs) <del class="diffchange diffchange-inline">are protonated </del>in the periplasmic space <del class="diffchange diffchange-inline">and </del>FadD facilitates <del class="diffchange diffchange-inline">its </del>transport into the cytosol.</td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td<ins class="diffchange diffchange-inline">> <b</ins>>Importation of long-chain fatty acids by FadL and FadD.<ins class="diffchange diffchange-inline"></b> </ins>Long chain fatty acids (LCFAs) in <ins class="diffchange diffchange-inline">the extracellular space are imported by the action of FadL protein into </ins>the periplasmic space<ins class="diffchange diffchange-inline">. Due to the low pH, LCFAs are are protonated. </ins>FadD <ins class="diffchange diffchange-inline">adds a CoA moiety to the carboxyl group, which </ins>facilitates <ins class="diffchange diffchange-inline">the </ins>transport <ins class="diffchange diffchange-inline">of LCFAs </ins>into the cytosol.</td></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="content2">The fadL gene codes for an outer membrane-bound fatty acids transport protein that facilitates the import of long chain fatty acids into the periplasmic space where they are protonated and then partitioned at the inner membrane (with the ionic head pointing towards the periplasmic space). Protonated fatty acids are unable to pass through the inner membrane into the cytosol and <del class="diffchange diffchange-inline">requires </del>the assistance of the FadD protein. The fadD gene codes for an inner membrane-associated long chain acyl-CoA synthetase enzyme in <i>E. coli</i> which catalyses the addition of a CoA moiety to the fatty <del class="diffchange diffchange-inline">acid </del>and <del class="diffchange diffchange-inline">its </del>subsequent transport across the inner membrane into the cytosol. FadD is therefore required both for the transport of LCFAs through the inner membrane into the cytosol and their activation for metabolism via the β-oxidation pathway. By overexpressing FadL and FadD, we aim to enhance LCFA uptake to boost the subsequent conversion of <del class="diffchange diffchange-inline">long chain </del>acyl-CoA to ALA.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="content2">The <ins class="diffchange diffchange-inline"><i></ins>fadL<ins class="diffchange diffchange-inline"></i> </ins>gene codes for an outer membrane-bound fatty acids transport protein that facilitates the import of long chain fatty acids into the periplasmic space where they are protonated and then partitioned at the inner membrane (with the ionic head pointing towards the periplasmic space). Protonated fatty acids are unable to pass through the inner membrane into the cytosol and <ins class="diffchange diffchange-inline">require </ins>the assistance of the FadD protein. The <ins class="diffchange diffchange-inline"><i></ins>fadD<ins class="diffchange diffchange-inline"></i> </ins>gene codes for an inner membrane-associated long chain acyl-CoA synthetase enzyme in <i>E. coli</i><ins class="diffchange diffchange-inline">, </ins>which catalyses the addition of a CoA moiety to the fatty <ins class="diffchange diffchange-inline">acids </ins>and <ins class="diffchange diffchange-inline">their </ins>subsequent transport across the inner membrane into the cytosol. FadD is therefore required both for the transport of LCFAs through the inner membrane into the cytosol and their activation for metabolism via the β-oxidation pathway. By overexpressing FadL and FadD, we aim to enhance LCFA uptake to boost the subsequent conversion of <ins class="diffchange diffchange-inline">fatty </ins>acyl-CoA to ALA.</div></td></tr>
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Kenlaukit
http://2014.igem.org/wiki/index.php?title=Team:CityU_HK/project/module_fad&diff=270169&oldid=prev
Marialilokyee at 11:27, 16 October 2014
2014-10-16T11:27:53Z
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Marialilokyee
http://2014.igem.org/wiki/index.php?title=Team:CityU_HK/project/module_fad&diff=270156&oldid=prev
Marialilokyee at 11:27, 16 October 2014
2014-10-16T11:27:37Z
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Marialilokyee
http://2014.igem.org/wiki/index.php?title=Team:CityU_HK/project/module_fad&diff=269561&oldid=prev
Marialilokyee at 10:56, 16 October 2014
2014-10-16T10:56:54Z
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Marialilokyee
http://2014.igem.org/wiki/index.php?title=Team:CityU_HK/project/module_fad&diff=266723&oldid=prev
Kenlaukit at 08:24, 16 October 2014
2014-10-16T08:24:55Z
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Kenlaukit