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Team:Carnegie Mellon/Protein
From 2014.igem.org
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<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Project_Description">Project Description</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Project_Description">Project Description</a></li> | ||
<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Our_Sensor">Our Sensor</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Our_Sensor">Our Sensor</a></li> | ||
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<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Achievements">Achievements</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Achievements">Achievements</a></li> | ||
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</ul> | </ul> | ||
</li> <li class="dropdown"> | </li> <li class="dropdown"> | ||
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<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Attributions">Attributions</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Attributions">Attributions</a></li> | ||
<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Sponsors">Sponsors</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Sponsors">Sponsors</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| - | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Policies & Practices <b class="caret"></b></a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/ | + | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Our_Projects">Our Projects</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/ | + | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Gallery">Gallery</a></li> |
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</ul> | </ul> | ||
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<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Weeks">Week by Week</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Weeks">Week by Week</a></li> | ||
<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Sensor">Sensor</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Sensor">Sensor</a></li> | ||
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<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Protein">Fluorescent Protein Evaluation</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Protein">Fluorescent Protein Evaluation</a></li> | ||
<li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Superoxide">Superoxide Generator Improvement</a></li> | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Superoxide">Superoxide Generator Improvement</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/InterLab">Interlab Study</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
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<h1><center>Fluorescent Protein Evaluation</center> </h1> | <h1><center>Fluorescent Protein Evaluation</center> </h1> | ||
| - | <p> <center>A set of five fluorescent proteins were analyzed to determine their signal to noise ratio. This method of quantification is meant to provide information about the amount of fluorescence that can be detected from the protein through the background fluorescence from other cellular components. | + | <center><p><img src ="https://static.igem.org/mediawiki/2014/9/9c/Screen_Shot_2014-10-17_at_1.01.30_AM.png" alt="FPs"</p></center> |
| + | <p>A set of five fluorescent proteins (blue, green, yellow, orange and red) were analyzed to determine their signal to noise ratio. This method of quantification is meant to provide information about the amount of fluorescence that can be detected from the protein through the background fluorescence from other cellular components. We used two cell lines (MACH and Top10) to determine the signal-to-noise ratio of the proteins in a cellular system.</p> | ||
<hr> | <hr> | ||
| - | + | <h2>Results</h2> | |
| - | + | <p>Based on the signal-to-noise ratios, which were maximized in the yellow, orange and red fluorescent proteins, we decided to use the yellow and red fluorescent proteins as reporters for our sensor. The crossover fluorescence analysis data was also used to designate the yellow and red fluorescent proteins as reporters. </p> | |
| + | <p><a href="https://static.igem.org/mediawiki/2014/2/2c/Screen_Shot_2014-10-16_at_1.29.56_PM.png">MACH Cells Crossover Fluorescence</a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/8/83/Screen_Shot_2014-10-16_at_1.29.54_PM.png">Top10 Cells Crossover Fluorescence</a></p> | ||
| + | <hr> | ||
| + | <h4>Fluorescent Proteins in MACH Cells</h4> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/c/c1/FP_MACH.png" height=600 width=600> | ||
| + | <hr> | ||
| + | <h4>Fluorescent Proteins in Top10 Cells</h4> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/9/9f/Top10_Best.jpg" height=600 width=600> | ||
| + | <hr> | ||
| + | <h4>Relative Magnitudes of S/N of Fluorescent Proteins in Different Cell Lines</h4> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/7/72/FP_Compare.png" height=600 width=600> | ||
| + | <hr> | ||
| + | <h3><center><green><a href="https://2014.igem.org/Team:Carnegie_Mellon/Weeks">Week by Week Notebook Entries</font></a></green></center></h3> | ||
Latest revision as of 17:45, 17 October 2014
Fluorescent Protein Evaluation
A set of five fluorescent proteins (blue, green, yellow, orange and red) were analyzed to determine their signal to noise ratio. This method of quantification is meant to provide information about the amount of fluorescence that can be detected from the protein through the background fluorescence from other cellular components. We used two cell lines (MACH and Top10) to determine the signal-to-noise ratio of the proteins in a cellular system.
Results
Based on the signal-to-noise ratios, which were maximized in the yellow, orange and red fluorescent proteins, we decided to use the yellow and red fluorescent proteins as reporters for our sensor. The crossover fluorescence analysis data was also used to designate the yellow and red fluorescent proteins as reporters.
MACH Cells Crossover Fluorescence
Top10 Cells Crossover Fluorescence
Fluorescent Proteins in MACH Cells
Fluorescent Proteins in Top10 Cells
Relative Magnitudes of S/N of Fluorescent Proteins in Different Cell Lines
