"
Team:Cambridge-JIC/Project
From 2014.igem.org
| Line 15: | Line 15: | ||
</div> | </div> | ||
| + | <!-- /.container --> | ||
| + | |||
| + | </div> | ||
| + | <!-- /.intro-header --> | ||
<!--Project Description--> | <!--Project Description--> | ||
Revision as of 13:51, 29 August 2014
Project Description
Sensing is an essential aspect of engineering. We need information about the world to make intelligent efforts to manipulate it. Ideal sensors are reliable, accurate and unobtrusive. Plants as biosensors fulfill these requirements and have the further benefit of being inexpensive and self-reproducing.
Our aim is to use the lower plant Marchantia polymorpha as a flexible, open source biosensor. The input, processing and output abilities are each parceled into separate modules which are linked using transcription factors and inducible promoters. Modules can be interchanged, so by swapping one input or output for another, PhytoSpy can flexibly be used to sense any condition or chemical, and report it in any way that a module has been designed for. This modular framework allows many devices to be constructed from the same library of components.
Our dream is to produce input, processing and output modules in separate plants which can be combined through Mendelian crossing. We want to make plant biosensors accessible to the home enthusiast in the same way that electronics has been made accessible by Arduino.
Constructs
35S - Chromoproteins
We've managed to build the following sequences correctly...
eforRed - a red chromoprotein
tsPurple - a purple chromoprotein
tsPurple-N7 - a purple chromoprotein localized to the nucleus
asPink-N7 - a pink chromoprotein localized to the nucleus
amilCP-N7 - a yellow chromoprotein localized to the nucleus
Now to be transformed into Marchantia.
35S - HAP1 - HAP1 UAS - Chromoproteins
Constructs and primers designed, primers to be checked and ordered.
35S - HAP1 - HAP1 UAS - Raspberry ketone
Benzalacetone synthase: sequence and sample from Japanese team
Benzalacetone reductase: primers designed from the Stanford biobrick, waiting for extraction kits to extract from raspberry plant.
Venus - HHR+Apt
35S - HAP1 - HHR+Apt - HAP1 UAS - Venus
Samples for two buffers, an inverter and a control sent by C. Smolke in a plate.
Primers to order today (04 Aug)
We now have theophylline and tetracycline.
Inducible promoter - GAL4 - GAL4 UAS - HAP1 - HAP1 UAS - Venus
List of nitrate, phosphate inducible promoters, clock genes, light related genes etc. and sequences of corrensponding proteins made. Comparison with tblastn to the marchantia genes done, now analysing the data to retain best candidates and get promoter sequences.
Dexamethasone - GAL4 - GAL4 UAS - HAP1 - HAP1 UAS - Venus
Full cycle - Salil says that we are not doing any Dexamethasone inducible constructs
Auxin inducible promoter - VenusN7
Hugh - initial research: I am trying to find a sample which contains the promoter
Project Description and History
Content
References
- Overall project summary
- Project Details
- Materials and Methods
- The Experiments
- Results
- Data analysis
- Conclusions
