Team:Cambridge-JIC/Guide/Constructs/Terminators

From 2014.igem.org

Cambridge iGEM 2014


Unfortunately, there is a lack of terminators in MP. The only one to find consistent use is the [http://parts.igem.org/Part:BBa_K1484215 nopaline synthase terminator].

This is a terminator found on the wild-type A. tumefaciens T-DNA!

This makes sequencing multi-gene constructs difficult. Slight variations to the sequence can be introduced via PCR, and mutiple repeats of the terminator can be added.