Team:CSU Fort Collins/Safety/

From 2014.igem.org

(Difference between revisions)
m
 
(24 intermediate revisions not shown)
Line 5: Line 5:
<title>Safety</title>
<title>Safety</title>
 +
 +
<head>
 +
<link href='http://fonts.googleapis.com/css?family=Bitter:400,700,400italic' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Bitter:400,700,400italic' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Muli:300,400' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Muli:300,400' rel='stylesheet' type='text/css'>
-
 
-
<body style="background-size:100%; background-color:#EFEFEF">
 
<style type="text/css">
<style type="text/css">
-
A{
+
/* http://meyerweb.com/eric/tools/css/reset/
-
    color:#222222;
+
  v2.0 | 20110126
 +
  License: none (public domain)
 +
*/
 +
 
 +
html, body, div, span, applet, object, iframe, p, blockquote, pre,
 +
a, abbr, acronym, address, big, cite, code,
 +
del, dfn, em, img, ins, kbd, q, s, samp,
 +
small, strike, strong, sub, sup, tt, var,
 +
b, u, i, center,
 +
dl, dt, dd, ol, ul, li,
 +
fieldset, form, label, legend,
 +
table, caption, tbody, tfoot, thead, tr, th, td,
 +
article, aside, canvas, details, embed,
 +
figure, figcaption, footer, header, hgroup,
 +
menu, nav, output, ruby, section, summary,
 +
time, mark, audio, video {
 +
  margin: 0;
 +
  padding: 0;
 +
  border: 0;
 +
  font-size: 90%;
 +
  font: inherit;
 +
  vertical-align: baseline;
}
}
-
A:visited{
+
/* HTML5 display-role reset for older browsers */
-
    color:#222222;
+
article, aside, details, figcaption, figure,
 +
footer, header, hgroup, menu, nav, section {
 +
  display: block;
}
}
-
A:hover{
+
body {
-
    opacity:0.3;
+
  line-height: 1;
-
    text-decoration:none;
+
}
}
-
h1,h2,h3,h4,h5,h6{
+
ol, ul {
-
    font-family:'Bitter', serif;
+
  list-style: none;
-
    text-decoration:none;
+
-
    font-weight:700;
+
-
    color:#222222;
+
-
    border:none;
+
}
}
-
.pic{
+
blockquote, q {
-
          width:100px;
+
  quotes: none;
-
          height:100px;
+
}
-
          border-radius:100%;
+
blockquote:before, blockquote:after,
-
    }
+
q:before, q:after {
-
p{
+
  content: '';
 +
  content: none;
 +
}
 +
table {
 +
  border-collapse: collapse;
 +
  border-spacing: 0;
 +
}
 +
 
 +
#menubar > ul{
 +
  vertical-align:middle;
 +
  font-size:14px;
 +
  font-family:'Times New Roman',serif;
 +
}
 +
 
 +
.firstHeading{
 +
display:none;
 +
}
 +
 
 +
#textbox span{
 +
  color:#E6B800;
 +
}
 +
.photo{
 +
  width:70%;
 +
}
 +
table{
 +
  font-family:'Muli',sans-serif;
 +
}
 +
body{
 +
  background-color:#EFEFEF;
 +
  line-height:1.3;
 +
  font-size:90%;
 +
}
 +
#cssmenu {
 +
  width:100%;
 +
  height: 44px;
 +
  background: #222222;
 +
  position:relative;
 +
  text-align:center;
 +
  margin:auto;
 +
}
 +
#cssmenu ul {
 +
  list-style: none;
 +
  padding: 0;
 +
  margin: 0;
 +
  line-height: 1;
 +
}
 +
#cssmenu > ul {
 +
  position: relative;
 +
  display: block;
 +
  background: #222222;
 +
  width: 100%;
 +
  z-index: 500;
 +
}
 +
#cssmenu:after,
 +
#cssmenu > ul:after {
 +
  content: '.';
 +
  display: block;
 +
  clear: both;
 +
  visibility: hidden;
 +
  line-height: 0;
 +
  height: 0;
 +
}
 +
#cssmenu.align-right > ul > li {
 +
  float: right;
 +
}
 +
#cssmenu.align-center ul {
 +
  text-align: center;
 +
}
 +
#cssmenu.align-center ul ul {
 +
  text-align: left;
 +
}
 +
#cssmenu > ul > li {
 +
  display: inline-block;
 +
  position: relative;
 +
  margin: 0;
 +
  padding: 0;
 +
}
 +
#cssmenu > ul > #menu-button {
 +
  display: none;
 +
}
 +
#cssmenu ul li a {
 +
  display: block;
 +
  font-family: Helvetica, sans-serif;
 +
  text-decoration: none;
 +
}
 +
#cssmenu > ul > li > a {
 +
  font-size: 14px;
 +
  font-weight: bold;
 +
  padding: 15px 20px;
 +
  color: #efefef;
 +
  text-transform: uppercase;
 +
  -webkit-transition: color 0.25s ease-out;
 +
  -moz-transition: color 0.25s ease-out;
 +
  -ms-transition: color 0.25s ease-out;
 +
  -o-transition: color 0.25s ease-out;
 +
  transition: color 0.25s ease-out;
 +
}
 +
#cssmenu > ul > li.has-sub > a {
 +
  padding-right: 32px;
 +
}
 +
#cssmenu > ul > li:hover > a {
 +
  color: #E6B800;
 +
}
 +
#cssmenu li.has-sub::after {
 +
  display: block;
 +
  content: '';
 +
  position: absolute;
 +
  width: 0;
 +
  height: 0;
 +
}
 +
#cssmenu > ul > li.has-sub::after {
 +
  right: 10px;
 +
  top: 20px;
 +
  border: 5px solid transparent;
 +
  border-top-color: #efefef;
 +
}
 +
#cssmenu > ul > li:hover::after {
 +
  border-top-color: #E6B800;
 +
}
 +
#indicatorContainer {
 +
  position: absolute;
 +
  height: 12px;
 +
  width: 100%;
 +
  bottom: 0px;
 +
  overflow: hidden;
 +
  z-index: -1;
 +
}
 +
#pIndicator {
 +
  position: absolute;
 +
  height: 0;
 +
  width: 100%;
 +
  border: 12px solid transparent;
 +
  border-top-color: #2b2f3a;
 +
  z-index: -2;
 +
  -webkit-transition: left .25s ease;
 +
  -moz-transition: left .25s ease;
 +
  -ms-transition: left .25s ease;
 +
  -o-transition: left .25s ease;
 +
  transition: left .25s ease;
 +
}
 +
#cIndicator {
 +
  position: absolute;
 +
  height: 0;
 +
  width: 100%;
 +
  border: 12px solid transparent;
 +
  border-top-color: #2b2f3a;
 +
  top: -12px;
 +
  right: 100%;
 +
  z-index: -2;
 +
}
 +
#cssmenu ul ul {
 +
  position: absolute;
 +
  left: -9999px;
 +
  top: 70px;
 +
  opacity: 0;
 +
  -webkit-transition: opacity .3s ease, top .25s ease;
 +
  -moz-transition: opacity .3s ease, top .25s ease;
 +
  -ms-transition: opacity .3s ease, top .25s ease;
 +
  -o-transition: opacity .3s ease, top .25s ease;
 +
  transition: opacity .3s ease, top .25s ease;
 +
  z-index: 1000;
 +
}
 +
#cssmenu ul ul ul {
 +
  top: 37px;
 +
  padding-left: 5px;
 +
}
 +
#cssmenu ul ul li {
 +
  position: relative;
 +
}
 +
#cssmenu > ul > li:hover > ul {
 +
  left: auto;
 +
  top: 44px;
 +
  opacity: 1;
 +
}
 +
#cssmenu.align-right > ul > li:hover > ul {
 +
  left: auto;
 +
  right: 0;
 +
  opacity: 1;
 +
}
 +
#cssmenu ul ul li:hover > ul {
 +
  left: 170px;
 +
  top: 0;
 +
  opacity: 1;
 +
}
 +
#cssmenu.align-right ul ul li:hover > ul {
 +
  left: auto;
 +
  right: 170px;
 +
  top: 0;
 +
  opacity: 1;
 +
  padding-right: 5px;
 +
}
 +
#cssmenu ul ul li a {
 +
  width: 130px;
 +
  border-bottom: 1px solid #eeeeee;
 +
  padding: 10px 20px;
 +
  font-size: 12px;
 +
  color: #222222;
 +
  background: white;
 +
  -webkit-transition: all .35s ease;
 +
  -moz-transition: all .35s ease;
 +
  -ms-transition: all .35s ease;
 +
  -o-transition: all .35s ease;
 +
  transition: all .35s ease;
 +
}
 +
#cssmenu.align-right ul ul li a {
 +
  text-align: right;
 +
}
 +
#cssmenu ul ul li:hover > a {
 +
  background: white;
 +
  color: #E6B800;
 +
}
 +
#cssmenu ul ul li:last-child > a,
 +
#cssmenu ul ul li.last > a {
 +
  border-bottom: 0;
 +
}
 +
#cssmenu > ul > li > ul::after {
 +
  content: '';
 +
  border: 6px solid transparent;
 +
  width: 0;
 +
  height: 0;
 +
  border-bottom-color: #efefef;
 +
  position: absolute;
 +
  top: -12px;
 +
  left: 30px;
 +
}
 +
#cssmenu.align-right > ul > li > ul::after {
 +
  left: auto;
 +
  right: 30px;
 +
}
 +
#cssmenu ul ul li.has-sub::after {
 +
  border: 4px solid transparent;
 +
  border-left-color: #222222;
 +
  right: 10px;
 +
  top: 12px;
 +
  -moz-transition: all .2s ease;
 +
  -ms-transition: all .2s ease;
 +
  -o-transition: all .2s ease;
 +
  transition: all .2s ease;
 +
  -webkit-transition: -webkit-transform 0.2s ease, right 0.2s ease;
 +
}
 +
#cssmenu.align-right ul ul li.has-sub::after {
 +
  border-left-color: transparent;
 +
  border-right-color: #222222;
 +
  right: auto;
 +
  left: 10px;
 +
}
 +
#cssmenu ul ul li.has-sub:hover::after {
 +
  border-left-color: #efefef;
 +
  right: -5px;
 +
  -webkit-transform: rotateY(180deg);
 +
  -ms-transform: rotateY(180deg);
 +
  -moz-transform: rotateY(180deg);
 +
  -o-transform: rotateY(180deg);
 +
  transform: rotateY(180deg);
 +
}
 +
#cssmenu.align-right ul ul li.has-sub:hover::after {
 +
  border-right-color: #efefef;
 +
  border-left-color: transparent;
 +
  left: -5px;
 +
  -webkit-transform: rotateY(180deg);
 +
  -ms-transform: rotateY(180deg);
 +
  -moz-transform: rotateY(180deg);
 +
  -o-transform: rotateY(180deg);
 +
  transform: rotateY(180deg);
 +
}
 +
@media all and (max-width: 800px), only screen and (-webkit-min-device-pixel-ratio: 2) and (max-width: 1024px), only screen and (min--moz-device-pixel-ratio: 2) and (max-width: 1024px), only screen and (-o-min-device-pixel-ratio: 2/1) and (max-width: 1024px), only screen and (min-device-pixel-ratio: 2) and (max-width: 1024px), only screen and (min-resolution: 192dpi) and (max-width: 1024px), only screen and (min-resolution: 2dppx) and (max-width: 1024px) {
 +
  #cssmenu {
 +
    width: 100%;
 +
  }
 +
  #cssmenu.align-center ul {
 +
    text-align: left;
 +
  }
 +
  #cssmenu.align-right > ul > li {
 +
    float: none;
 +
  }
 +
  #cssmenu ul {
 +
    width: auto;
 +
  }
 +
  #cssmenu .submenuArrow,
 +
  #cssmenu #indicatorContainer {
 +
    display: none;
 +
  }
 +
  #cssmenu > ul {
 +
    height: auto;
 +
    display: block;
 +
    color:#EFEFEF;
 +
  }
 +
  #cssmenu > ul > li {
 +
    float: none;
 +
  }
 +
  #cssmenu li,
 +
  #cssmenu > ul > li {
 +
    display: none;
 +
    color:white;
 +
  }
 +
  #cssmenu ul ul,
 +
  #cssmenu ul ul ul,
 +
  #cssmenu ul > li:hover > ul,
 +
  #cssmenu ul ul > li:hover > ul,
 +
  #cssmenu.align-right ul ul,
 +
  #cssmenu.align-right ul ul ul,
 +
  #cssmenu.align-right ul > li:hover > ul,
 +
  #cssmenu.align-right ul ul > li:hover > ul {
 +
    position: relative;
 +
    left: auto;
 +
    top: auto;
 +
    opacity: 1;
 +
    padding-left: 0;
 +
    padding-right: 0;
 +
    right: auto;
 +
  }
 +
  #cssmenu ul .has-sub::after {
 +
    display: none;
 +
  }
 +
  #cssmenu ul li a {
 +
    padding: 12px 20px;
 +
  }
 +
  #cssmenu ul ul li a {
 +
    border: 0;
 +
    background: none;
 +
    width: auto;
 +
    padding: 8px 35px;
 +
    color:white;
 +
  }
 +
  #cssmenu.align-right ul ul li a {
 +
    text-align: left;
 +
  }
 +
  #cssmenu ul ul li:hover > a {
 +
    background:none;
 +
    color: #ec9359;
 +
  }
 +
  #cssmenu ul ul ul a {
 +
    padding: 8px 50px;
 +
  }
 +
  #cssmenu ul ul ul ul a {
 +
    padding: 8px 65px;
 +
  }
 +
  #cssmenu ul ul ul ul ul a {
 +
    padding: 8px 80px;
 +
  }
 +
  #cssmenu ul ul ul ul ul ul a {
 +
    padding: 8px 95px;
 +
  }
 +
  #cssmenu > ul > #menu-button {
 +
    display: block;
 +
    cursor: pointer;
 +
  }
 +
  #cssmenu #menu-button > a {
 +
    padding: 14px 20px;
 +
  }
 +
  #cssmenu ul.open li,
 +
  #cssmenu > ul.open > li {
 +
    display: block;
 +
  }
 +
  #cssmenu > ul.open > li#menu-button > a {
 +
    color: #fff;
 +
    border-bottom: 1px solid rgba(150, 150, 150, 0.1);
 +
  }
 +
  #cssmenu ul ul::after {
 +
    display: none;
 +
  }
 +
  #cssmenu #menu-button::after {
 +
    display: block;
 +
    content: '';
 +
    position: absolute;
 +
    height: 3px;
 +
    width: 22px;
 +
    border-top: 2px solid #efefef;
 +
    border-bottom: 2px solid #efefef;
 +
    right: 20px;
 +
    top: 15px;
 +
  }
 +
  #cssmenu #menu-button::before {
 +
    display: block;
 +
    content: '';
 +
    position: absolute;
 +
    height: 3px;
 +
    width: 22px;
 +
    border-top: 2px solid #efefef;
 +
    right: 20px;
 +
    top: 25px;
 +
  }
 +
  #cssmenu ul.open #menu-button::after,
 +
  #cssmenu ul.open #menu-button::before {
 +
    border-color: #fff;
 +
  }
 +
}
 +
.icon{
 +
  width:30px;
 +
}
 +
.wrapper2{
 +
  width:20%;
 +
  padding:20px 0px 20px 20px;
 +
  display:inline-block;
 +
  margin:auto;
 +
  vertical-align:top;
 +
}
 +
.wrapper{
 +
  width:90%;
 +
  padding:20px 0px 20px 20px;
 +
  padding-left:0;
 +
  margin:auto;
 +
  vertical-align: top;
 +
}
 +
.page, .container{
 +
  width:50%;
 +
  min-width:600px;
 +
  max-width:800px;
 +
  padding:30px;
 +
  margin:auto;
 +
  text-align:left;
 +
  font-family:'Muli',sans-serif;
 +
  display:inline-block;
 +
}
 +
.page a, .container a{
 +
  color:#222222;
 +
}
 +
.page a:hover, .container a:hover{
 +
  color:#E6B800;
 +
  text-decoration:none;
 +
}
 +
 
 +
h1{
 +
  color:#222222;
 +
  font-family:'Bitter',serif;
 +
  border-bottom:none;
 +
}
 +
h2,h3,.toc,.show,.hide,.content{
 +
  color:#E6B800;
 +
  font-family:'Bitter',serif;
 +
}
 +
h4,h5,h6, .page{
 +
  color:#222222;
 +
  font-family:'Muli',sans-serif;
 +
}
 +
.toc{
 +
  text-decoration:none;
 +
  display:none;
 +
  width:100%;
 +
  margin:auto;
 +
}
 +
.toc .month-group { margin: 20px 0; }
 +
.toc .month-group p { padding: 0; margin: 0; }
 +
#ftoc{
 +
  color:#666666;
 +
}
 +
ul{
 +
  list-style-type:none;
 +
}
 +
.month-group, a{
 +
  text-decoration:none;
 +
}
 +
.month-group{
 +
  margin-left:20px;
 +
}
 +
.month-group:hover{
 +
  color:#E6B800;
 +
}
 +
.toc a, .toc2 a, #navi a{
 +
  text-decoration:none;
 +
  color:#222222;
 +
  font-weight: bold;
 +
}
 +
.toc a:hover, #ftoc>a:hover, #navi a:hover, .show:hover, .hide:hover, .container>a:hover{
 +
  color:#E6B800 !important;
 +
  cursor:pointer;
 +
}
 +
.toc a, #ftoc, .container a, #navi{
 +
  border-bottom:2px dotted;
 +
}
 +
#navi{
 +
  text-decoration:none;
 +
  font-family:'Bitter',serif;
 +
}
 +
#logo{
 +
  width:120px;
 +
  margin:auto;
 +
  padding:40px;
 +
  border-radius:100%;
 +
}
 +
#banner{
 +
  width:auto;
 +
  height:250px;
 +
  vertical-align:top;
 +
  background-color:#E6B800;
 +
  overflow: hidden
 +
}
 +
.fixed {
 +
  position:fixed !important;
 +
  top:0;
 +
}
 +
.disp{
 +
  display:none;
 +
}
 +
.col {
 +
    width: 20%;
 +
    float: left;
 +
    clear: right;
 +
    margin: 20px 1.66666666666666666666666666%;
     font-family:'Muli',sans-serif;
     font-family:'Muli',sans-serif;
     font-weight:300;
     font-weight:300;
 +
    font-size:14px;
     color:#222222;
     color:#222222;
 +
    text-align:left;
}
}
-
 
+
.clear{
-
td{
+
  width:100%;
-
    text-align:center;
+
  clear:both;
-
    padding:30px;
+
-
    font-family:Helvetica, Arial, sans-serif;
+
-
    font-size:15px;
+
}
}
-
 
+
.container{
-
.button{
+
  line-height:1.9;
-
    display:inline;
+
-
    margin-left:15px;
+
-
    margin-right:15px;
+
-
    padding:10px;
+
-
    border-radius:5px;
+
-
    font-family:'Bitter', serif;
+
-
    font-size:15px;
+
}
}
-
ul ul {
+
.index{
-
display: none;
+
  margin-right:20px;
 +
}
 +
.toc2{
 +
  display:block;
 +
}
 +
.footer{
 +
    background-color:#222222;
 +
    width:100%;
 +
    margin:auto;
 +
    padding:15px;
 +
    color:#efefef;
 +
    font-size:14px;
 +
}
 +
.footer table{
 +
    background-color:#222222;
 +
    color:#efefef;
 +
    margin:auto;
 +
}
 +
.footer a{
 +
    color:#efefef;
 +
}
 +
#banner a:hover{
 +
opacity:0.8;
}
}
-
ul li:hover > ul {
 
-
display: block;
 
-
}
 
</style>
</style>
-
<!---navigation bar--->
+
</head>
-
<center><div style="width:90%; padding:0px 30px">
 
-
<ul>
 
-
<li class="button"><a href="/Team:CSU_Fort_Collins">Home</a></li>
+
 
-
<li class="button"><a href="/Team:CSU_Fort_Collins/Team/">Team</a></li>
+
<body style='margin:0'>
-
    <ul>
+
 
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/Team/">Member Profiles</a></li>
+
<div id='banner'><center><img src='https://static.igem.org/mediawiki/2014/4/43/Team-CSU_Safety.jpg' style='width:100%; margin:-350px; min-width:1000px'/><a href='https://2014.igem.org'><img src='https://static.igem.org/mediawiki/2014/d/d7/Sysuchina_igemdeLogo.png' style="width:80px; position:absolute; top: 10px; right: 20px; opacity:0.95;"/></a>
-
    <li class="button"><a href="https://igem.org/Team.cgi">Official Team Profile</a></li>
+
</center></div>
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/Sponsors/">Sponsors</a></li>
+
 
-
    </ul>
+
<div id='cssmenu'>
-
<li class="button"><a href="/Team:CSU_Fort_Collins/Project/">Project</a></li>
+
<ul>
-
    <ul>
+
  <li><a href='/Team:CSU_Fort_Collins'><span>Home</span></a></li>
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/Biosensor/">Biosensor</a></li>
+
  <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Project/'><span>Project</span></a>
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/Breakdown/">Breakdown</a></li>
+
      <ul>
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/HVP/">High-Value Product</a></li>
+
        <li><a href='/Team:CSU_Fort_Collins/Biosensor/'><span>Biosensor</span></a></li>
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/KillSwitch/">Kill Switch</a></li>
+
        <li><a href='/Team:CSU_Fort_Collins/Breakdown/'><span>Breakdown</span></a></li>
-
    </ul>
+
        <li><a href='/Team:CSU_Fort_Collins/HVP/'><span>High-Value Product</span></a></li>
-
<li class="button"><a href="/Team:CSU_Fort_Collins"><img src="https://static.igem.org/mediawiki/2014/0/03/CSULogo1.png" style="width:200px"/></a></li>
+
        <li><a href='/Team:CSU_Fort_Collins/KillSwitch/'><span>Kill Switch</span></a></li>
-
<li class="button"><a href="/Team:CSU_Fort_Collins/Outreach/">Outreach</a></li>
+
      </ul>
-
    <ul>
+
  </li>
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/Collaboration/">Collaboration</a></li>
+
  <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Members/'><span>Team</span></a>
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/Events/">Events</a></li>
+
      <ul>
-
    <li class="button"><a href="/Team:CSU_Fort_Collins/GMO/">GMO Outreach</a></li>
+
        <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li>
-
    </ul>
+
        <li><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li>
-
<li class="button"><a href="/Team:CSU_Fort_Collins/Safety/">Safety</a></li>
+
        <li class='last'><a href='/Team:CSU_Fort_Collins/Acknowledgements/'><span>Acknowledgements</span></a></li>
-
<li class="button"><a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" style="height:50px"/></a></li>
+
      </ul>
 +
  </li>
 +
  <li class='has-sub' class='active'><a href='/Team:CSU_Fort_Collins/Notebook/'><span>Notebook</span></a>
 +
    <ul style="display:block">
 +
      <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Protocols'><span>Protocols</span></a>
 +
        <ul>
 +
          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Gibson"><span>Gibson Assembly</span></a></li>
 +
          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Cloning"><span>Cloning Genes</span></a></li>
 +
          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Miniprep"><span>Plasmid Miniprep</span></a></li>
 +
          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Isolation"><span>Yeast DNA Isolation</span></a></li>
 +
          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Gel"><span>Gel Electrophoresis</span></a></li>
 +
          <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Purify"><span>PCR Product Purification</span></a></li>
 +
        </ul>
 +
      </li>
 +
      <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/DailyNotes'><span>Daily Notes</span></a>
 +
        <ul>
 +
        <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun'><span>Biosensor</span></a>
 +
            <ul>
 +
              <li><a href="/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun"><span>June</span></a></li>
 +
              <li><a href="/Team:CSU_Fort_Collins/Notebook/Biosensor/Jul"><span>July</span></a></li>
 +
              <li class='last'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Aug'><span>August</span></a></li>
 +
            </ul>
 +
        </li>
 +
        <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'><span>Breakdown</span></a>
 +
            <ul>
 +
              <li><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul"><span>July</span></a></li>
 +
              <li><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Aug'><span>August</span></a></li>
 +
              <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep"><span>September</span></a></li>
 +
            </ul>
 +
        </li>
 +
        <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/Jun'><span>High-Value Product</span></a>
 +
            <ul>
 +
              <li><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jun"><span>June</span></a></li>
 +
              <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jul"><span>July</span></a></li>
 +
            </ul>
 +
        </li>
 +
        <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul'><span>Kill Switch</span></a>
 +
            <ul>
 +
              <li><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul"><span>July</span></a></li>
 +
              <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Sep"><span>September</span></a></li>
 +
            </ul>
 +
        </li>
 +
      </ul>
 +
  </li>
 +
  </ul>
 +
  </li>
 +
  <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Collab/'><span>Human Practices</span></a>
 +
      <ul>
 +
        <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li>
 +
        <li class='last'><a href='/Team:CSU_Fort_Collins/Outreach/'><span>Outreach</span></a></li>
 +
      </ul>
 +
  </li>
 +
  <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Parts/'><span>Achievements</span></a>
 +
      <ul>
 +
        <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li>
 +
        <li class='last'><a href='/Team:CSU_Fort_Collins/Judging/'><span>Judging</span></a></li>
 +
      </ul>
 +
  </li>
 +
  <li class='last'><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
</ul>
</ul>
 +
</div>
-
<br>
+
 
-
<br>
+
 
 +
<div class="clear"></div>
 +
 
 +
<div class='wrapper'>
 +
  <center>
 +
    <h1>Safety</h1>
 +
 
 +
    <div class="container">
 +
      <h3>Safety in the Lab</h3>
 +
 
 +
      Our team members all completed safety training regarding general laboratory safety, the responsible code of research, working with and handling biosafety level 1 and 2 agents, and hazardous waste safety and disposal. To reduce risks to personnel in the lab as well as the general public, we have completed these trainings and used simple laboratory strains with biosafety level 1 risk. Additionally, we wear proper personal proptective equipment (PPE) when working in lab. More about these requirements and training can be found <a href='http://www.ehs.colostate.edu/WBiosafety/PDF/POSTED_CSU_Biosafety_Manual.pdf'>here</a>.
 +
  <br><br>
 +
      Biosafety at Colorado State University is managed by our Environmental Health Services (EHS) and the Institutional Biosafety Committee. We follow all regulations and training requirements as required for all team members.
 +
      More on CSU EHS and their policies can be found on their <a href='http://www.ehs.colostate.edu/'>website</a>.
 +
      <br><br>
 +
      Biosafety in the United States is governed by the Center for Disease Control (CDC). More on their regulations can be seen <a href='http://www.cdc.gov/biosafety/publications/bmb15/bmbl.pdf'>here</a>.
 +
<br><br>
 +
      <h3>Organisms Utillized</h3>
 +
  The two organisms we used (or used parts of) were:<br>
 +
          <u>Escherichia coli</u> K-12 DH5&#945;<br>
 +
  <u>Saccharomyces cerevisiae</u> S288C<br>
 +
<table style='background-color:#efefef'>
 +
<tr>
 +
<td style='padding:10px; vertical-align:middle'><img src='https://static.igem.org/mediawiki/2014/6/61/Team-CSU_Ecoli.jpg' style='width:300px'/></td>
 +
<td style='padding:10px; vertical-align:middle'><img src='https://static.igem.org/mediawiki/2014/a/a2/Team-CSU_Yeast.jpg' style='width:300px'/></td>
 +
</tr>
 +
<tr>
 +
<td style='text-align:center'> <u>E. coli</u> </td>
 +
<td style='text-align:center'> <u>S. cerevisiae</u> </td>
 +
</tr>
 +
</table>
 +
  <br>
 +
  For a complete list of the hazards associated with these organisms and our specific use of them, see our <a href='https://2014.igem.org/File:CSU_Fort_Collins_Safety2014_Spreadsheet.xls'>Safety Spreadsheet</a>.
 +
 
 +
<br><br>
 +
      <h3>Risks of Our Project Now</h3>
 +
      <h4>Risks to team members</h4>
 +
      Small safty risks such as eye irritation or irritation of the respiratory tract exist when using <u>E. coli</u> in the lab. Our team has been trained to minimize these risks and appropriate PPE is always utilized in the lab.<br>
 +
      <h4>Risks to the general public</h4>
 +
      While the organisms we use are resistant to some antibiotics, the strains are laboratory strains not found in the natural environment. Thus, a release of these organisms would be unlikely to survive outside the lab environment and should pose no significant threat to the health of the general public.
 +
      <br>
 +
      <h4>Risks to the environment</h4>
 +
      All biological materials are autoclaved before disposal and all waste disposal, hazardous and biological, is handled by trained lab members as follows university and United States requirements. Any biological materials to escape from our lab pose little to no risk to the environment, as they would be very unlikely to survive outside a lab.
 +
<br><br>
 +
      <h3>Risks of Our Project in the Future</h3>
 +
      <h4>Risks arising from our project's growth</h4>
 +
      Antibiotic-resistant and genetically modified <u>E. coli</u> has the potential to infect laboratory workers or the general public. However, this situations is unlikely, as our organisms have little survival success in non-laboratory environments. Testing would need to be done to confirm that the use of the terpenoid produced via the <u>E. coli</u> was safe for the environment (e.g. ensure that the use in a natural environment does not have toxic or dangerous effects). We do not suspect that there would be any malicious mis-use of our research or components. The knowledge and methods we use are currently widely available and are not dangerous as long as safety precautions are always taken.
 +
      <br><br>
 +
 
 +
      <h4>Reducing Risks</h4>
 +
      Risk of release is reduced or eliminated completely by the addition of the kill switch biobrick which induces apoptosis in modified cells which are introduced to light (e.g. outside). Physical containment would also be a key safety feature in a commercialized or widely-distributed version of our final project and components. Most likely, we would keep the organism in a bioreactor, closed container, or laboratory setting at all times.
 +
 
 +
 
 +
      <br><br><br>
 +
      <center>Find our official safety form <a href='https://igem.org/Safety/Safety_Form?team_id=1495'>here</a>.</center>
 +
    </div>
 +
 
 +
  </center>
</div>
</div>
-
</center>
+
 
 +
 
 +
<script type="text/javascript" src="https://code.jquery.com/jquery-2.1.1.min.js"></script>
 +
<script type='text/javascript'>
 +
  $('#cssmenu').prepend('<div id="indicatorContainer"><div id="pIndicator"><div id="cIndicator"></div></div></div>');
 +
      var activeElement = $('#cssmenu>ul>li:first');
 +
 
 +
      $('#cssmenu>ul>li').each(function() {
 +
          if ($(this).hasClass('active')) {
 +
              activeElement = $(this);
 +
          }
 +
      });
 +
 
 +
 
 +
    var posLeft = activeElement.position().left;
 +
    var elementWidth = activeElement.width();
 +
    posLeft = posLeft + elementWidth/2 -6;
 +
    if (activeElement.hasClass('has-sub')) {
 +
      posLeft -= 6;
 +
    }
 +
 
 +
    $('#cssmenu #pIndicator').css('left', posLeft);
 +
    var element, leftPos, indicator = $('#cssmenu pIndicator');
 +
   
 +
    $("#cssmenu>ul>li").hover(function() {
 +
          element = $(this);
 +
          var w = element.width();
 +
          if ($(this).hasClass('has-sub'))
 +
          {
 +
            leftPos = element.position().left + w/2 - 12;
 +
          }
 +
          else {
 +
            leftPos = element.position().left + w/2 - 6;
 +
          }
 +
 
 +
          $('#cssmenu #pIndicator').css('left', leftPos);
 +
      }
 +
      , function() {
 +
        $('#cssmenu #pIndicator').css('left', posLeft);
 +
      });
 +
 
 +
    $('#cssmenu>ul').prepend('<li id="menu-button"><a>Menu</a></li>');
 +
    $( "#menu-button" ).click(function(){
 +
          if ($(this).parent().hasClass('open')) {
 +
            $(this).parent().removeClass('open');
 +
          }
 +
          else {
 +
            $(this).parent().addClass('open');
 +
          }
 +
        });
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
    $(document).on('click', '.show', function(){
 +
          $('.toc').show(100);
 +
          $('.show').hide();
 +
    });
 +
    $(document).on('click', '.hide', function(){
 +
          $('.toc').hide();
 +
          $('.show').show(100);
 +
    });
 +
});
 +
</script>
 +
<script type='text/javascript'>
 +
  $(document).ready(function() {
 +
 
 +
    //Calculate the height of <header>
 +
    //Use outerHeight() instead of height() if have padding
 +
    var aboveHeight = $('#banner').height();
 +
 
 +
//when scroll
 +
    $(window).scroll(function(){
 +
 
 +
        //if scrolled down more than the header’s height
 +
            if ( $(window).scrollTop() > aboveHeight ){
 +
 
 +
        // if yes, add “fixed” class to the <nav>
 +
        // add padding top to the #content
 +
            //(value is same as the height of the nav)
 +
            $('#cssmenu').addClass('fixed').next().css('padding-top','44');
 +
           
 +
 
 +
            } else {
 +
 
 +
        // when scroll up or less than aboveHeight,
 +
            //remove the “fixed” class, and the padding-top
 +
            $('#cssmenu').removeClass('fixed').next().css('padding-top','0');
 +
         
 +
            }
 +
 
 +
            if ( $(window).scrollTop() > aboveHeight-14 ){
 +
              $('#top-section').addClass('disp');
 +
            } else{
 +
            $('#top-section').removeClass('disp');
 +
            }
 +
        });
 +
    });
 +
 
 +
</script>
 +
 
</body>
</body>
 +
</html>
</html>

Latest revision as of 18:12, 17 October 2014

Safety

Safety

Safety in the Lab

Our team members all completed safety training regarding general laboratory safety, the responsible code of research, working with and handling biosafety level 1 and 2 agents, and hazardous waste safety and disposal. To reduce risks to personnel in the lab as well as the general public, we have completed these trainings and used simple laboratory strains with biosafety level 1 risk. Additionally, we wear proper personal proptective equipment (PPE) when working in lab. More about these requirements and training can be found here.

Biosafety at Colorado State University is managed by our Environmental Health Services (EHS) and the Institutional Biosafety Committee. We follow all regulations and training requirements as required for all team members. More on CSU EHS and their policies can be found on their website.

Biosafety in the United States is governed by the Center for Disease Control (CDC). More on their regulations can be seen here.

Organisms Utillized

The two organisms we used (or used parts of) were:
Escherichia coli K-12 DH5α
Saccharomyces cerevisiae S288C
E. coli S. cerevisiae

For a complete list of the hazards associated with these organisms and our specific use of them, see our Safety Spreadsheet.

Risks of Our Project Now

Risks to team members

Small safty risks such as eye irritation or irritation of the respiratory tract exist when using E. coli in the lab. Our team has been trained to minimize these risks and appropriate PPE is always utilized in the lab.

Risks to the general public

While the organisms we use are resistant to some antibiotics, the strains are laboratory strains not found in the natural environment. Thus, a release of these organisms would be unlikely to survive outside the lab environment and should pose no significant threat to the health of the general public.

Risks to the environment

All biological materials are autoclaved before disposal and all waste disposal, hazardous and biological, is handled by trained lab members as follows university and United States requirements. Any biological materials to escape from our lab pose little to no risk to the environment, as they would be very unlikely to survive outside a lab.

Risks of Our Project in the Future

Risks arising from our project's growth

Antibiotic-resistant and genetically modified E. coli has the potential to infect laboratory workers or the general public. However, this situations is unlikely, as our organisms have little survival success in non-laboratory environments. Testing would need to be done to confirm that the use of the terpenoid produced via the E. coli was safe for the environment (e.g. ensure that the use in a natural environment does not have toxic or dangerous effects). We do not suspect that there would be any malicious mis-use of our research or components. The knowledge and methods we use are currently widely available and are not dangerous as long as safety precautions are always taken.

Reducing Risks

Risk of release is reduced or eliminated completely by the addition of the kill switch biobrick which induces apoptosis in modified cells which are introduced to light (e.g. outside). Physical containment would also be a key safety feature in a commercialized or widely-distributed version of our final project and components. Most likely, we would keep the organism in a bioreactor, closed container, or laboratory setting at all times.


Find our official safety form here.

Retrieved from "http://2014.igem.org/Team:CSU_Fort_Collins/Safety/"