Team:CSU Fort Collins/Notebook/

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Revision as of 02:54, 14 October 2014

CSU iGEM 2014

Table of Contents

1

Protocols

01 Gibson Assembly
02 Cloning Genes
03 Plasmid Miniprep
04 Yeast DNA Extraction
05 Gel Electrophoresis
06 PCR Product Purification

2

Daily Notes

Biosensor

07June: PAR, PLR, and PFL Assembly
08July: PLR, PFL, and PFL2 Assembly
09August: Scientists vs. the PCR Machine

Breakdown

11July: Lac Promoter and FadD Assembly
12August: More of the Same, But Better
13September: One Thousand and One Nights

High-Value Product

14June: pMBI Assembly
15July: pMBI and pMevT Assembly
16August: Troubleshoot Everything!
17September: Plan B

Kill Switch

18July: TRP/KillerRed Assembly
19August: The Month of Failed Transformations
20September: Troubleshooting and Biobrick Success

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