Team:Braunschweig/Project

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         <title>E. Cowli - Fighting Climate Change - iGEM 2014 Team Braunschweig</title>
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         <title>E.&nbsp;cowli - Fighting Climate Change - iGEM 2014 Team Braunschweig</title>
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<div class="pagesummary"><a name="Summary" class="anchor"></a>
<div class="pagesummary"><a name="Summary" class="anchor"></a>
<h1>Summary</h1>
<h1>Summary</h1>
-
<p style="text-align:justify;">As a result of the increased greenhouse effect the Earth&rsquo;s climate is changing noticeably. Methane being a greenhouse gas like carbon dioxide (CO2) traps heat in the atmosphere increasing the global warming. However, it has to be kept in mind that methane&rsquo;s impact on the greenhouse effect is 25-fold greater compared to CO2.<br />
+
<p style="text-align:justify;">As a result of the enhanced greenhouse effect the Earth&rsquo;s climate is changing noticeably. Methane, a greenhouse gas like carbon dioxide (CO<sub>2</sub>), traps heat in the atmosphere increasing the global warming. However, it has to be kept in mind that the impact of methane on the greenhouse effect is 25-fold greater compared to CO<sub>2</sub>.<br />
-
One of several natural sources of methane is enteric fermentation in ruminants such as milk cows and cattle. In their rumen, billions of microorganisms release greenhouse gases such as carbon dioxide and methane as they help to digest the animal&rsquo;s food. This is, in fact, a natural process, but industrial-scale farming and increasing demand intensify the emissions and with that the greenhouse effect. Although more and more people are willing to permanently do without dairy products and beef, the majority does not. <br />
+
One of the several natural sources of methane is enteric fermentation in ruminants such as milk cows and cattle. In their rumen, billions of microorganisms release greenhouse gases as they help digesting the animal&rsquo;s food. This is, in fact, a natural process, but industrial-scale farming and increasing demand intensify the emissions and thus the greenhouse effect. Although more and more people are willing to permanently do without dairy products and beef, the majority does not. <br />
-
Methane can be utilised as a carbon source by so called methanotrophic bacteria one of them being the modelorganism Methylococcus capsulatus. M. capsulatus uses the the well characterised soluble methane monooxygenase (sMMO), an enzyme complex consisting of three subunits, to convert methane to methanol.<br />
+
Methane can be utilized as a carbon source by methanotrophic bacteria, among them the model organism <i>Methylococcus capsulatus. M.&nbsp;capsulatus</i> uses the well-characterized soluble methane monooxygenase (sMMO), an enzyme complex consisting of three subunits, to convert methane to methanol.<br />
-
Considering the huge impact of methane in comparison to CO2, methane emissions are a substantial point of action for future climate protection and thus gave us the idea for our project: We are going to enable an engineered bacterium to metabolize methane just at its place of origin - the cow&rsquo;s rumen.<br />
+
Considering the huge impact of methane in comparison to CO<sub>2</sub>, methane emissions are a substantial point of action for future climate protection and thus gave us the idea for our project: We designed an engineered bacterium to metabolize methane right at its place of origin - the cow&rsquo;s rumen.<br />
-
During this iGEM season we were able to develop a technique to successfully express all components of the methane monooxygenase in E. coli in a soluble form and we furthermore proofed the activity of the complete enzyme complex. After construction and transformation of our final device and optimization of the cultivation conditions, we suceeded to produce one of the sMMO&rsquo;s subunits, MMOC, solubly and another one, MMOX, in inclusion bodies (see figure 1). In order to achieve effective production of the remaining subunits in the soluble fraction we further analysed cotransformation of our engineered E.coli strain and different combinations of known chaperone proteins. Our findings suggest that the expression of the sMMO works best when coexpressed with the bacterial chaperones GroEL and GroES (see figure 2). For reasons of protection against bacteria consuming ciliates in rumen fluid and thus to enable survival of E.cowli we also tested entrapment and cultivation in alginate beads (figure 3). Using a methane sensor we were also able to show degradation of methane by our engineered bacterium E.cowli.<br />
+
During this iGEM season we were able to develop a technique to successfully express all components of the methane monooxygenase in <i>E.&nbsp;coli</i> in soluble form and we, furthermore, proved the activity of the complete enzyme complex. After construction and transformation of our final device and optimization of the cultivation conditions, we succeeded to produce one of the sMMO&rsquo;s subunits, MMOC, in soluble form and another one, MMOX, in inclusion bodies. To achieve effective production of the remaining subunits in the soluble fraction we further analysed cotransformation of our engineered <i>E.&nbsp;coli</i> strain with different combinations of known chaperone proteins. Our findings suggest that the expression of the sMMO works best when coexpressed with the bacterial chaperones GroEL and GroES. For reasons of protection against bacteria-consuming ciliates in rumen fluid and thus to enable survival of <i>E.&nbsp;cowli</i>, we also tested entrapment and cultivation in alginate beads. Using a methane sensor we were also able to show degradation of methane by our engineered bacterium <i> E.&nbsp;cowli</i>.<br />
-
To evaluate the efficiency of E.cowli in the natural environment of the cow&rsquo;s rumen, we generated a mathematical model based on experimentally achieved and literature data. Hence, we figured out that a total of 50 dollars per year is needed to reduce the annual methane emission by 110 kg per cow. Our mathematical model clearly indicates that a significant amount of methane can be degraded directly in the cow&rsquo;s rumen when E.cowli is added to the feed. That implies that the greenhouse gas methane issued from enteric fermentation is no longer released into the Earth&rsquo;s atmosphere and thus can no longer contribute to the greenhouse effect. Future plans are to obtain purified sMMO in appropriate amounts in order to turn it into an industrially useful product. The new usability of the sMMO conquers three major concerns of the industrialized economy: pollution, shortage of energy and of course global warming. <br />
+
To evaluate the efficiency of <i>E.&nbsp;cowli</i> in the natural environment of the cow&rsquo;s rumen, we generated a mathematical model based on experimentally achieved and literature data. Hence, we figured out that as little as 50 $US per year is sufficient to reduce the annual methane emission by 110 kg per cow. Our mathematical model clearly indicates that a significant amount of methane can be degraded directly in the cow&rsquo;s rumen when <i>E.&nbsp;cowli</i> is added to the feed. This implies that the greenhouse gas methane arising from enteric fermentation would no longer be released into the Earth&rsquo;s atmosphere and could thus no longer contribute to the greenhouse effect. Future plans are to obtain purified sMMO in appropriate amounts to turn it into an industrially useful product. The new usability of the sMMO could conquer three major concerns of the industrialized economy: pollution, shortage of energy and of course global warming. <br />
-
As the sMMO is able to degrade a broad variety of substrates such as one of the major water pollutants, the industrial solvent trichlorethylene (TCE), our project can serve as a foundation for a new approach of biological water treatment or environmental care in general. <br />
+
As the sMMO is able to degrade a broad variety of substrates such as one of the major water pollutants, the industrial solvent trichloroethylene (TCE), our project can serve as a foundation for a new approach of biological water treatment or environmental care in general. <br />
-
One could also exploit the sMMO&rsquo;s ability to convert methane to methanol: Methane can serve as a source of energy but due to its gaseous state it is very difficult to transport compared to <br />
+
One could also exploit the sMMO&rsquo;s ability to convert methane to methanol: Methane can serve as a source of energy, but due to its gaseous state it is very difficult to transport compared to the liquid methanol. Up to now methane is chemically converted to methanol for transportation and later again chemically converted to components of diesel fuel and gasoline or to propylene and ethylene, precursors of important chemical substances. Using the sMMO to oxidize methane is a much more economical and eco-friendly approach to utilize methane as a resource for energy production.<br />
-
the liquid methanol. Up to now methane is chemically converted to methanol for transportation and later again chemically converted to components of diesel fuel and gasoline or to propylene and ethylene, important precursors of important chemical substances. Using the sMMO to oxidize methane is a much more economical and eco-friendly approach to utilize methane as a resource for energy production.<br />
+
Hence, our project has a huge significance regarding a wide-ranging spectrum of problems and opportunities. <br />   
Hence, our project has a huge significance regarding a wide-ranging spectrum of problems and opportunities. <br />   
</p>
</p>
<p>
<p>
-
This year's iGEM team of the Technische Universität Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to engineer a bacterium able to metabolise methane just at its place of origin - the rumen of the cow. The well characterised and easily manageable model organism <i>Escherichia coli</i> is therefore planned to be equipped with an enzyme complex, the methane monooxygenase (MMO), which turns the greenhouse gas methane into a a natural intermediate of the cellular metabolism. This way, no methane would be released into the atmosphere. The six subunits of the MMO as well as other potentially required proteins will be incorporated into the new <i>E. cowli</i> bacterium. This would allow for the worldwide methane emissions to be reduced and, considering the constant population growth, for future food supply to be rendered more climate friendly.
 
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     <span class="box_header">Problem</span><br/>
     <span class="box_header">Problem</span><br/>
     <p class="box_text">
     <p class="box_text">
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The methane emissions from bovine and dairy cattle contribute to greenhouse effect and climate change.
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The methane emissions from bovine and dairy cattle contribute to the greenhouse effect and climate change.
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     <span class="box_header">Idea</span><br/>
     <span class="box_header">Idea</span><br/>
     <p class="box_text">
     <p class="box_text">
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In this year's project we will fight climate change right at one of its most important sources - the rumen of cattle!
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In this year's project, we will fight climate change right at one of its most important sources - the rumen of cattle!
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     </p>
     </div>
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     <span class="box_header">Approach</span><br/>
     <span class="box_header">Approach</span><br/>
     <p class="box_text">
     <p class="box_text">
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In our approach we make use of a naturally occurring enzyme and a well-known model organism. Get further information here!
+
In our approach, we make use of a naturally occurring enzyme and a well-known model organism. Get further information here!
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   <div class="col-md-5"><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Results"><img class="box_img" style="width: 400px; margin-left: 5px;"
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   <div class="col-md-5"><a href="https://2014.igem.org/Team:Braunschweig/Results"><img class="box_img" style="width: 400px; margin-left: 5px;"
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src="https://static.igem.org/mediawiki/2014/a/a9/TU-BS_Home_Project.jpg" alt="Kuh"/></a>
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src="https://static.igem.org/mediawiki/2014/9/92/TU-BS_Results-Titel-Kuh.png" alt="Kuh"/></a>
   </div>
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   <div class="col-md-7" style="
   <div class="col-md-7" style="
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     width: 630px;">
<h1>Results</h1></a>
<h1>Results</h1></a>
-
   <p class="box_text">The iGEM Team Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to equip the model organsim <i>Escherichia coli</i> with the methane monooxygenase (MMO), an enzyme complex enabling methaotrophs to use the greenhouse gas methane as sole source of carbon and energy, hence creating our methane degrading <i>E. cowli</i>.
+
   <p class="box_text">The iGEM Team Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to equip the model organism <i>E. coli</i> with the methane monooxygenase (sMMO), an enzyme complex enabling methanotrophs to use the greenhouse gas methane as a sole source of carbon and energy, hence creating our methane-degrading <i>E.&nbsp;cowli</i>.
   </p>
   </p>
   </div>
   </div>
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                                 <p class="box_text">
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“With great power comes great responsibility.” - Uncle Ben (and Voltaire)
+
“With great power comes great responsibility.” - Uncle Ben (and Voltaire)<br>
-
On the basis of this quote we thought about other possible applications of our <i>E. cowli</i>.
+
On the basis of this quote, we thought about other possible applications of our <i>E.&nbsp;cowli</i>.
                                 </p>
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                                 <p class="box_text">
                                 <p class="box_text">
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During the design and implementation of our project we always kept in mind fome fundamental principles of engineering and synthetic biology.
+
During the design and implementation of our project, we always kept in mind some fundamental principles of engineering and synthetic biology.
                                 </p>
                                 </p>
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  <div class="height30"></div>
  <div class="height30"></div>
                         <div class="footer_bottom_content">Copyright © 2014 iGEM Team Braunschweig<br>
                         <div class="footer_bottom_content">Copyright © 2014 iGEM Team Braunschweig<br>
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<a href="#">Contact us</a></div>
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<a href="mailto:igem.braunschweig@gmail.com">Contact us</a></div>
                          
                          
                     </div>
                     </div>

Latest revision as of 21:02, 17 October 2014

E. cowli - Fighting Climate Change - iGEM 2014 Team Braunschweig

Summary

As a result of the enhanced greenhouse effect the Earth’s climate is changing noticeably. Methane, a greenhouse gas like carbon dioxide (CO2), traps heat in the atmosphere increasing the global warming. However, it has to be kept in mind that the impact of methane on the greenhouse effect is 25-fold greater compared to CO2.
One of the several natural sources of methane is enteric fermentation in ruminants such as milk cows and cattle. In their rumen, billions of microorganisms release greenhouse gases as they help digesting the animal’s food. This is, in fact, a natural process, but industrial-scale farming and increasing demand intensify the emissions and thus the greenhouse effect. Although more and more people are willing to permanently do without dairy products and beef, the majority does not.
Methane can be utilized as a carbon source by methanotrophic bacteria, among them the model organism Methylococcus capsulatus. M. capsulatus uses the well-characterized soluble methane monooxygenase (sMMO), an enzyme complex consisting of three subunits, to convert methane to methanol.
Considering the huge impact of methane in comparison to CO2, methane emissions are a substantial point of action for future climate protection and thus gave us the idea for our project: We designed an engineered bacterium to metabolize methane right at its place of origin - the cow’s rumen.
During this iGEM season we were able to develop a technique to successfully express all components of the methane monooxygenase in E. coli in soluble form and we, furthermore, proved the activity of the complete enzyme complex. After construction and transformation of our final device and optimization of the cultivation conditions, we succeeded to produce one of the sMMO’s subunits, MMOC, in soluble form and another one, MMOX, in inclusion bodies. To achieve effective production of the remaining subunits in the soluble fraction we further analysed cotransformation of our engineered E. coli strain with different combinations of known chaperone proteins. Our findings suggest that the expression of the sMMO works best when coexpressed with the bacterial chaperones GroEL and GroES. For reasons of protection against bacteria-consuming ciliates in rumen fluid and thus to enable survival of E. cowli, we also tested entrapment and cultivation in alginate beads. Using a methane sensor we were also able to show degradation of methane by our engineered bacterium E. cowli.
To evaluate the efficiency of E. cowli in the natural environment of the cow’s rumen, we generated a mathematical model based on experimentally achieved and literature data. Hence, we figured out that as little as 50 $US per year is sufficient to reduce the annual methane emission by 110 kg per cow. Our mathematical model clearly indicates that a significant amount of methane can be degraded directly in the cow’s rumen when E. cowli is added to the feed. This implies that the greenhouse gas methane arising from enteric fermentation would no longer be released into the Earth’s atmosphere and could thus no longer contribute to the greenhouse effect. Future plans are to obtain purified sMMO in appropriate amounts to turn it into an industrially useful product. The new usability of the sMMO could conquer three major concerns of the industrialized economy: pollution, shortage of energy and of course global warming.
As the sMMO is able to degrade a broad variety of substrates such as one of the major water pollutants, the industrial solvent trichloroethylene (TCE), our project can serve as a foundation for a new approach of biological water treatment or environmental care in general.
One could also exploit the sMMO’s ability to convert methane to methanol: Methane can serve as a source of energy, but due to its gaseous state it is very difficult to transport compared to the liquid methanol. Up to now methane is chemically converted to methanol for transportation and later again chemically converted to components of diesel fuel and gasoline or to propylene and ethylene, precursors of important chemical substances. Using the sMMO to oxidize methane is a much more economical and eco-friendly approach to utilize methane as a resource for energy production.
Hence, our project has a huge significance regarding a wide-ranging spectrum of problems and opportunities.

Project

Problem

The methane emissions from bovine and dairy cattle contribute to the greenhouse effect and climate change.

Idea

In this year's project, we will fight climate change right at one of its most important sources - the rumen of cattle!

Approach

In our approach, we make use of a naturally occurring enzyme and a well-known model organism. Get further information here!

Kuh

Results

The iGEM Team Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to equip the model organism E. coli with the methane monooxygenase (sMMO), an enzyme complex enabling methanotrophs to use the greenhouse gas methane as a sole source of carbon and energy, hence creating our methane-degrading E. cowli.

  • Potential Impact
  • Potential Impact

    “With great power comes great responsibility.” - Uncle Ben (and Voltaire)
    On the basis of this quote, we thought about other possible applications of our E. cowli.

  • Engineering Principles
  • Engineering Principles

    During the design and implementation of our project, we always kept in mind some fundamental principles of engineering and synthetic biology.

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