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Team:Braunschweig
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| - | <title>E. | + | <title>E. cowli - Fighting Climate Change - iGEM 2014 Team Braunschweig</title> |
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<li id="navbarhome"><a href="https://2014.igem.org/Team:Braunschweig">Home</a></li> | <li id="navbarhome"><a href="https://2014.igem.org/Team:Braunschweig">Home</a></li> | ||
<li id='navbarteam'><a href="https://2014.igem.org/Team:Braunschweig/Team">Team</a> | <li id='navbarteam'><a href="https://2014.igem.org/Team:Braunschweig/Team">Team</a> | ||
<ul id="nav-dd" style="margin-left:-50px"> | <ul id="nav-dd" style="margin-left:-50px"> | ||
| - | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Team#profiles">Profiles</a></li> | |
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<li><a href="https://2014.igem.org/Team:Braunschweig/Team-content#Collaborations">Collaboration</a></li> | <li><a href="https://2014.igem.org/Team:Braunschweig/Team-content#Collaborations">Collaboration</a></li> | ||
<li><a href="https://2014.igem.org/Team:Braunschweig/Gallery">Gallery</a></li> | <li><a href="https://2014.igem.org/Team:Braunschweig/Gallery">Gallery</a></li> | ||
<li><a href="https://2014.igem.org/Team:Braunschweig/Team-content#Attributions">Attributions</a></li> | <li><a href="https://2014.igem.org/Team:Braunschweig/Team-content#Attributions">Attributions</a></li> | ||
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| + | <li><a target="_blank" href="https://igem.org/Team.cgi?year=2014&team_name=Braunschweig">Official Team Profile</a></li> | ||
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<li id='navbarproject'><a href="https://2014.igem.org/Team:Braunschweig/Project">Project</a> | <li id='navbarproject'><a href="https://2014.igem.org/Team:Braunschweig/Project">Project</a> | ||
<ul id="nav-dd" style="margin-left:-105px"> | <ul id="nav-dd" style="margin-left:-105px"> | ||
| - | <li><a | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Project">Summary</a></li> |
<li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Problem">Problem</a></li> | <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Problem">Problem</a></li> | ||
<li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Idea">Idea</a></li> | <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Idea">Idea</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content# | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Approach">Approach</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Braunschweig/ | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Results">Results</a></li> |
<li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Potential Impact">Impact</a></li> | <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Potential Impact">Impact</a></li> | ||
<li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Engineering Principles">Engineering Principles</a></li> | <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Engineering Principles">Engineering Principles</a></li> | ||
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<li id="navbarmodeling"><a href="https://2014.igem.org/Team:Braunschweig/Modeling">Modeling</a> | <li id="navbarmodeling"><a href="https://2014.igem.org/Team:Braunschweig/Modeling">Modeling</a> | ||
<ul id="nav-dd" style="margin-left:-80px"> | <ul id="nav-dd" style="margin-left:-80px"> | ||
| - | <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling# | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling-content#Modeling Approach">Modeling Approach</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling# | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling-content#MM">Mathematical Model</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling# | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling-content#Code">Code</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
<li id="navbarnotebook"><a href="https://2014.igem.org/Team:Braunschweig/Notebook">Notebook</a> | <li id="navbarnotebook"><a href="https://2014.igem.org/Team:Braunschweig/Notebook">Notebook</a> | ||
<ul id="nav-dd" style="margin-left:-80px"> | <ul id="nav-dd" style="margin-left:-80px"> | ||
| - | <li><a href="https://2014.igem.org/Team:Braunschweig/Notebook# | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Notebook-content#experiments">Experiments</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Braunschweig/Notebook# | + | <li><a href="https://2014.igem.org/Team:Braunschweig/Notebook-content#protocols">Protocols</a></li> |
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<div class="container"> | <div class="container"> | ||
<div class="carousel-caption"> | <div class="carousel-caption"> | ||
| - | <h1><i>E. | + | <h1><i style="font-size:50px">E. cowli</i></h1> |
| - | <p style="font-size: | + | <p style="font-size:47px">Fighting Climate Change at the Source</p> |
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<div class="carousel-caption"> | <div class="carousel-caption"> | ||
<div class="col-sm-6 col-md-6" style="margin-top:50px"> | <div class="col-sm-6 col-md-6" style="margin-top:50px"> | ||
| - | <h1><i>Kofi Annan</i></h1> | + | <h1><i style="font-size:38px">Kofi Annan</i></h1> |
| - | <p>On climate change, we often don't fully appreciate that it is a problem. We think it is a problem waiting to happen.</p> | + | <p style="font-size:30px">"On climate change, we often don't fully appreciate that it is a problem. We think it is a problem waiting to happen."</p> |
</div> | </div> | ||
<div class="col-sm-6 col-md-6" style="margin-top:50px"> | <div class="col-sm-6 col-md-6" style="margin-top:50px"> | ||
| - | <h1><i>Bill Gates</i></h1> | + | <h1><i style="font-size:38px">Bill Gates</i></h1> |
| - | <p>Climate change is a terrible problem, and it absolutely needs to be solved. It deserves to be a huge priority.</p> | + | <p style="font-size:30px">"Climate change is a terrible problem, and it absolutely needs to be solved. It deserves to be a huge priority."</p> |
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<div class="container"> | <div class="container"> | ||
<div class="carousel-caption"> | <div class="carousel-caption"> | ||
| - | <h1><i>Bill Nye</i></h1> | + | <h1><i style="font-size:45px">Bill Nye</i></h1> |
| - | <p>Climate change is happening, humans are causing it, and I think this is perhaps the most serious environmental issue facing us. </p> | + | <p style="font-size:43px">"Climate change is happening, humans are causing it, and I think this is perhaps the most serious environmental issue facing us."</p> |
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</div> | </div> | ||
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<div class="container"> | <div class="container"> | ||
<div class="pagesummary"> | <div class="pagesummary"> | ||
| - | <h1><i>E. | + | <h1><i>E. cowli</i> - Fighting climate change at the source </h1> |
| - | <p class="mr" style="text-align: | + | <p class="mr" style="text-align:justify;">Dairy and beef production are important to our daily lives and consumption needs. However, there are serious climate change issues regarding the continual expansion of these industries. The natural microbiota inside the cow’s rumen releases dangerous amounts of greenhouse gases as they help digest the animal's food. Therefore, finding ways to decrease the production of greenhouse gases of dairy cows and cattle is essential in fighting global warming. </p> |
| - | < | + | <h3 align="center">In this year’s iGEM project, we fight global warming right at the source - the cow's rumen.</h3><br> |
| - | In this year’s iGEM project we fight global warming right at | + | <p class="mr" style="text-align:justify;">Using the powerful tools of synthetic biology we make it possible to reduce the methane levels through a genetically engineered bacterium named <i>E. cowli</i>, using the soluble form of the enzyme complex methane monooxygenase (sMMO) for hydroxylation of methane to methanol. We successfully expressed all the subunits in soluble form and, furthermore, were able to prove the activity of the whole enzyme complex. Moreover, the effectivity, feasibility as well as the safety of this project was evaluated using a mathematical model. <br><br> |
| + | Besides the laboratory-related topics, we also initialized many more activities to spread the idea of synthetic biology and iGEM. On various occasions we had the chance to present ourselves and our project to the broad public. We taught synthetic biology not only in a lecture but also 1-on-1 in a hands-on internship with a pupil and we also used these events for evaluation of the people's opinions on synthetic biology and consumption of animal products. In many discussions with other teams we took the chance to debate iGEM-related topics such as ethics, safety and intellectual property.<br> | ||
| + | Get started and find out more about us and our project!</p> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="container section"> | <div class="container section"> | ||
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<a href="https://2014.igem.org/Team:Braunschweig/Team"> | <a href="https://2014.igem.org/Team:Braunschweig/Team"> | ||
| - | <img class="box_img" src="https://static.igem.org/mediawiki/2014/ | + | <img class="box_img" src="https://static.igem.org/mediawiki/2014/3/36/TU-BS_Home_Team.jpg" alt="gallery1"/></a> |
</div> | </div> | ||
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<p class="box_text"> | <p class="box_text"> | ||
| - | We are this | + | We are this year's iGEM Team of the Technische Universität Braunschweig. Get to know us, our motivations and much more on our team profiles! |
| - | </p | + | </p> |
</div> | </div> | ||
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<div> | <div> | ||
<a href="https://2014.igem.org/Team:Braunschweig/HP"> | <a href="https://2014.igem.org/Team:Braunschweig/HP"> | ||
| - | <img class="box_img" src="https://static.igem.org/mediawiki/2014/ | + | <img class="box_img" src="https://static.igem.org/mediawiki/2014/1/11/TU-BS_Home_HP.jpg" alt="Oli and Maren"/> |
</a> | </a> | ||
</div> | </div> | ||
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<span class="box_header">Policy & Practices</span><br/> | <span class="box_header">Policy & Practices</span><br/> | ||
<p class="box_text"> | <p class="box_text"> | ||
| - | + | iGEM is more than only working in a lab - it's spreading the word. Find out more about our events and actions on our Policy and Practices page. | |
</p> | </p> | ||
</div> | </div> | ||
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<div class="col-md-4"> | <div class="col-md-4"> | ||
<div class="cbox"> | <div class="cbox"> | ||
| - | <div | + | <div><a href="https://2014.igem.org/Team:Braunschweig/Modeling"> |
| - | + | <img class="box_img" src="https://static.igem.org/mediawiki/2014/3/3a/TU-BS_Home_Modeling.jpg" alt="gallery1"/> | |
| - | <img class="box_img" src="https://static.igem.org/mediawiki/2014/ | + | |
</a> | </a> | ||
</div> | </div> | ||
<div> | <div> | ||
| - | <span class="box_header"> | + | <span class="box_header">Modeling</span><br/> |
<p class="box_text"> | <p class="box_text"> | ||
| - | + | To evaluate the feasibilty of our project before has become reality we generated | |
| - | + | generated a mathematical model based on laboratory and literature data. | |
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="container section"> | <div class="container section"> | ||
<div class="cbox"> | <div class="cbox"> | ||
| - | <div class="row"> | + | <div class="row" style=" |
| - | <div class="col-md-5"><img class="box_img" src="https://static.igem.org/mediawiki/2014/ | + | width: 1100px;"> |
| + | <div class="col-md-5"><a href="https://2014.igem.org/Team:Braunschweig/Project"><img class="box_img" style="width: 400px; margin-left: 5px;" | ||
| + | src="https://static.igem.org/mediawiki/2014/a/a9/TU-BS_Home_Project.jpg" alt="Kuh"/></a> | ||
</div> | </div> | ||
| - | <div class="col-md-7"> | + | <div class="col-md-7" style=" |
| + | width: 630px;"> | ||
<h1>Project</h1></a> | <h1>Project</h1></a> | ||
| - | <p class="box_text">The iGEM Team Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to equip the model | + | <p class="box_text">The iGEM Team Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to equip the model organism <i>Escherichia coli</i> with the soluble methane monooxygenase (sMMO), an enzyme complex enabling methanotrophs to use the greenhouse gas methane as sole source of carbon and energy, hence creating our methane degrading <i>E. cowli</i>. |
</p> | </p> | ||
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<div class="cbox"> | <div class="cbox"> | ||
<div> | <div> | ||
| - | <a href="https://2014.igem.org/Team:Braunschweig/ | + | <a href="https://2014.igem.org/Team:Braunschweig/Safety"> |
| - | <img class="box_img" src="https://static.igem.org/mediawiki/2014/4/48/ | + | <img class="box_img" src="https://static.igem.org/mediawiki/2014/4/48/TU-BS_Home_Safety.JPG" alt="gallery1"/> |
</a> | </a> | ||
</div> | </div> | ||
<div> | <div> | ||
| - | <span class="box_header"> | + | <span class="box_header">Safety</span><br/> |
<p class="box_text"> | <p class="box_text"> | ||
| - | + | While designing our project, safety naturally was a very important issue for this year’s iGEM project. Issues in biosafety as well as gas health and safety were addressed. | |
| - | + | ||
| - | + | ||
</p> | </p> | ||
</div> | </div> | ||
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<div> | <div> | ||
<a href="https://2014.igem.org/Team:Braunschweig/Notebook"> | <a href="https://2014.igem.org/Team:Braunschweig/Notebook"> | ||
| - | <img class="box_img" src="https://static.igem.org/mediawiki/2014/ | + | <img class="box_img" src="https://static.igem.org/mediawiki/2014/5/50/TU-BS_Home_Notebook.jpg" alt="Oli and Maren"/> |
</a> | </a> | ||
</div> | </div> | ||
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<span class="box_header">Notebook</span><br/> | <span class="box_header">Notebook</span><br/> | ||
<p class="box_text"> | <p class="box_text"> | ||
| - | Everyone who is interested in how we spent our iGEM summer, feel free to have a look at our Notebook, where | + | Everyone who is interested in how we spent our iGEM summer, feel free to have a look at our Notebook, where we put together a documentation of our lab work. |
</p> | </p> | ||
</div> | </div> | ||
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<div class="img_circle"> | <div class="img_circle"> | ||
<a href="https://2014.igem.org/Team:Braunschweig/Achievements"> | <a href="https://2014.igem.org/Team:Braunschweig/Achievements"> | ||
| - | <img class="box_img" src="https://static.igem.org/mediawiki/2014/ | + | <img class="box_img" src="https://static.igem.org/mediawiki/2014/1/13/TU-BS_Home_Achievements.jpg" alt="gallery1"/> |
</a> | </a> | ||
</div> | </div> | ||
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<span class="box_header">Achievements</span><br/> | <span class="box_header">Achievements</span><br/> | ||
<p class="box_text"> | <p class="box_text"> | ||
| - | By contributing BioBricks to the iGEM Registry of Standard Biological | + | By contributing BioBricks to the iGEM Registry of Standard Biological Parts so that other teams can use them in the future, we live up to the basic ideas of the iGEM competition. |
</p> | </p> | ||
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</li> | </li> | ||
<li> | <li> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/ | + | <img src="https://static.igem.org/mediawiki/2014/0/08/TU-BS_Logo_Uni.jpg" height="60px" class="logo-sponsor" alt="TU Braunschweig Logo" /> |
</li> | </li> | ||
<li> | <li> | ||
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<li> | <li> | ||
<img src="https://static.igem.org/mediawiki/2014/b/b9/TU-BS_logo_neb.png" height="60px" class="logo-sponsor" alt="NEB Logo" /> | <img src="https://static.igem.org/mediawiki/2014/b/b9/TU-BS_logo_neb.png" height="60px" class="logo-sponsor" alt="NEB Logo" /> | ||
| + | </li> | ||
| + | <li> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/3/3f/TU-BS_logo_bhb.png" height="60px" class="logo-sponsor" alt="BHB Logo" /> | ||
| + | </li> | ||
| + | <li> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/e/e8/TU-BS_logo_itubs.png" height="60px" class="logo-sponsor" alt="iTUBS Logo" /> | ||
</li> | </li> | ||
</ul> | </ul> | ||
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<div class="height30"></div> | <div class="height30"></div> | ||
<div class="footer_bottom_content">Copyright © 2014 iGEM Team Braunschweig<br> | <div class="footer_bottom_content">Copyright © 2014 iGEM Team Braunschweig<br> | ||
| - | <a href=" | + | <a href="mailto:igem.braunschweig@gmail.com">Contact us</a></div> |
</div> | </div> | ||
Latest revision as of 20:24, 17 October 2014
E. cowli - Fighting climate change at the source
Dairy and beef production are important to our daily lives and consumption needs. However, there are serious climate change issues regarding the continual expansion of these industries. The natural microbiota inside the cow’s rumen releases dangerous amounts of greenhouse gases as they help digest the animal's food. Therefore, finding ways to decrease the production of greenhouse gases of dairy cows and cattle is essential in fighting global warming.
In this year’s iGEM project, we fight global warming right at the source - the cow's rumen.
Using the powerful tools of synthetic biology we make it possible to reduce the methane levels through a genetically engineered bacterium named E. cowli, using the soluble form of the enzyme complex methane monooxygenase (sMMO) for hydroxylation of methane to methanol. We successfully expressed all the subunits in soluble form and, furthermore, were able to prove the activity of the whole enzyme complex. Moreover, the effectivity, feasibility as well as the safety of this project was evaluated using a mathematical model.
Besides the laboratory-related topics, we also initialized many more activities to spread the idea of synthetic biology and iGEM. On various occasions we had the chance to present ourselves and our project to the broad public. We taught synthetic biology not only in a lecture but also 1-on-1 in a hands-on internship with a pupil and we also used these events for evaluation of the people's opinions on synthetic biology and consumption of animal products. In many discussions with other teams we took the chance to debate iGEM-related topics such as ethics, safety and intellectual property.
Get started and find out more about us and our project!
Team
We are this year's iGEM Team of the Technische Universität Braunschweig. Get to know us, our motivations and much more on our team profiles!
Policy & Practices
iGEM is more than only working in a lab - it's spreading the word. Find out more about our events and actions on our Policy and Practices page.
Modeling
To evaluate the feasibilty of our project before has become reality we generated generated a mathematical model based on laboratory and literature data.
Project
The iGEM Team Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to equip the model organism Escherichia coli with the soluble methane monooxygenase (sMMO), an enzyme complex enabling methanotrophs to use the greenhouse gas methane as sole source of carbon and energy, hence creating our methane degrading E. cowli.
Safety
While designing our project, safety naturally was a very important issue for this year’s iGEM project. Issues in biosafety as well as gas health and safety were addressed.
Notebook
Everyone who is interested in how we spent our iGEM summer, feel free to have a look at our Notebook, where we put together a documentation of our lab work.
Achievements
By contributing BioBricks to the iGEM Registry of Standard Biological Parts so that other teams can use them in the future, we live up to the basic ideas of the iGEM competition.
